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The Johns Hopkins Applied Imaging Mass Spectrometry – AIMS Core/Service Center

The Johns Hopkins AIMS Core/Service Center makes available rapid matrix-assisted laser desorption/ionization (MALDI) imaging at high spatial resolution, which includes sample preparation, on-tissue digests and derivatizations, and data analysis. The new AIMS Core, directed by Dr. Kristine Glunde, is housed in the Cancer Research 2 building (CRBII) in the lower basement in room LB03D and LB03E.

Spatially resolved MALDI imaging measurements are directly taken from a frozen or formalin-fixed paraffin-embedded (FFPE) tissue section without destroying it. MALDI imaging combines mass spectrometric analyses of biomolecules with simultaneous histological evaluation to analyze intact proteins, tryptic peptides (on-tissue tryptic digest), N-glycans (on-tissue PNGase digest), peptides, lipids, metabolites, and drug molecules in a spatially resolved manner.

mouse scan

Equipment

  • Bruker Rapiflex MALDI TOF/TOF instrument for high-throughput MALDI imaging
  • HTX M5 Sprayer for accurate robotic spraying of enzymes and matrices
  • Leica Cryostat for MALDI-imaging-compatible cryosectioning
  • Slide scanner for histology and immunohistochemistry co-registered with MALDI imaging
  • Workstation with SCiLS lab for data analysis

Services

  • MALDI-imaging compatible cryo-sectioning in gelatin and other MALDI-compatible cryo-media
  • MALDI-imaging sample preparation of formalin-fixed paraffin-embedded tissue sections
  • On-tissue digestions including for tryptic peptides and glycans
  • On-tissue derivatizations for metabolite imaging
  • Robotic matrix application with HTX M5 sprayer
  • High-throughput multiplexed MALDI imaging of up to 5,000 biomolecules at once
  • High spatial resolution MALDI imaging of 20 micron pixel size or better
  • MALDI imaging covering maximum area of regular microscopy slide (75 mm by 25 mm)
  • Customized development of MALDI imaging protocols
  • Targeted MALDI imaging of drugs, drug metabolites, imaging agents, contrast agents, or other agents
  • Discovery MALDI imaging of metabolites, lipids, peptides, intact proteins, tryptic peptides, glycans
  • On tissue MS/MS for analyte identification in imaging or profiling mode
  • Data analysis: segmentation analysis, pathology-guided analysis, and statistical analysis with dedicated software package (SCiLS Lab)

More information

 

Contact Information

For more information, please contact:

Dr. Kristine Glunde
The Russell H. Morgan Department of Radiology and Radiological Science
The Johns Hopkins University School of Medicine
Office: Traylor Building, Room 203, Rutland Avenue, Baltimore, Maryland 21205
AIMS Core: Cancer Research Building II, Room LB03D/E, Baltimore, Maryland 21231

Leadership

Kristine Glunde, M.S., Ph.D.​
Director
Email: kglunde1@jhmi.edu
Telephone: 410-614-2705 (office), 410-614-7959 (AIMS Core)