Translational Research and Innovation Laboratory (TRAIL)

Translational Research and Innovation Laboratory (TRAIL) was created in 2023 with the mission to bridge the gap between patients and laboratory science through teamwork and innovation.

The TRAIL is focused on answering questions raised by our clinical and clinical research colleagues. The capabilities of our laboratory are solely guided by the clinical and clinical and translational research needs of our Johns Hopkins All Children’s Hospital clinical investigators, as well as by investigators from collaborating institutions, with the aim to improve outcomes for all children. The TRAIL model was published in Nature Medicine in January 2026.  Nature Medicine Journal

The TRAIL team consists of laboratory-trained scientists and clinical investigators, and our success is based on leveraging a breadth of expertise and experience of individual team members, for robust solutions and innovations that would otherwise not be possible.

TRAIL leadership team

Neil Goldenberg MD, PhD

Professor of Pediatrics
Neil A Goldenberg

Vera Ignjatovic PhD, MBA

Adjunct Professor of Pediatrics
Vera Ignjatovic PhD, MBA

Steven Bruzek MS, MB

Program Manager
Steven Bruzek MS, MB

Trail Laboratory Staff

Grant Vestal

Research Specialist Senior
Grant Vestal Research JHACH

TRAIL investigators

Marisol Betensky, MD, MPH

Assistant Professor of Pediatrics
Marisol Betensky, M.D.

John Morrison, MD, PhD

Assistant Professor of Pediatrics
John Michael Morrison, M.D., Ph.D.

Anthony Alexander Sochet, M.D., M.H.S.

Assistant Professor of Anesthesiology and Critical Care Medicine
Anthony Sochet M.D.

Amy Kiskaddon PharmD, MBA

Assistant Professor of Pediatrics
Amy Kiskaddon

Maha Al-Ghafry, M.D.

Assistant Professor of Pediatrics
Maha Al-Ghafry MD

Michelle Hojnicki, D.O.

Assistant Professor of Pediatrics
Michelle Hojnicki DO

Alexa Marzo, M.D.

All Children's Specialty Physicians
Alexa Marzo, M.D.

Overview of Core Capabilities

Caption -The hub-and-spoke model of TRAIL at the Johns Hopkins All Children’s Hospital.

Capabilities listed by research area and/or methodological approach

Research Area Capabilities
Hemostasis
  • Simultaneous Thrombin and Plasmin Generation Assay
  • Clot Formation and Lysis (CloFAL) Assay
  • Modified mini-Euglobulin Clot Lysis Assay (modified mini-ECLA)
  • Novel assay development and refinement
Proteomics
  • Mass Dynamics Platform - Interactive proteomics data interpretation
  • Collaborations with external Mass Spectrometry Labs
    • Tandem Mass Tag (TMT) Proteomics
    • Data Independent Acquisition (DIA) Proteomics
  • Experimental Design Consultation (discovery to validation)
Whole Genome Sequencing  Promethion P2 Solo - Long read sequencing 
Microbiology 
  • Serum Associated Tolerance (SAT) Assay
  • Culture propagation and storage, growth assays, antibiotic susceptibility testing
  • Quantitative Viral Load Testing of Nasal Swabs
Project Support / Specimen Management 
  • Consultation for project/grant applications from planning stages through execution
    • Implementation, feasibility, budgeting
    • Best practices for collection, processing, storage and use of precious biological specimens, specifically for research involving neonates, infants, children and adolescents
    • Connection with existing capabilities and expertise
    • Planning through execution

We collaborate with National and International partners, including global leaders in laboratory diagnostics, as well as pharma, in a hub and spoke model of operation (see image).

Hub and Spoke Model Final

We are committed to mentoring and education of the next generation/s of translational researchers in the form of summer interns, and Clinical and Translational Research Training Track (CTRT) mentees.

Research Education & Training | Johns Hopkins Medicine

MENTEES

  A. General

  • Elizabeth Havlicek, DO, PCCM Fellow, University of Utah

  B. Clinical and Translational Research Training Track (CTRT)

  - Johns Hopkins All Children's Hospital

  • Kayla Delaney, MD, Pediatric Resident
  • Brett Russi, MD, PCCM Fellow
  • Audrey Hughes, MD, Pediatric Resident
  • Caitlin Crosier, MD, Surgery Fellow

    - Other
  • Rita Wyrebek, MD, MFNI Faculty, St Louis University, Missouri

 

Interns

  • Juliana Hodge (past)
  • Lauren White (past)
  • Matthew Danielson (past)

Funding

  • NIH
  • Johns Hopkins All Children’s Foundation

Contact