a man removes samples from a liquid nitrogen tank
a man removes samples from a liquid nitrogen tank
a man removes samples from a liquid nitrogen tank

Ovarian Tissue Freezing (Cryopreservation)

What You Need to Know

  • Freezing tissue from the ovaries can provide a chance for future fertility and hormone production in children and adults undergoing treatment for cancer or other diseases that affect the ovaries.
  • The egg-producing tissue from the ovary is removed, frozen and stored until the patient has completed treatment and wishes to become pregnant or have normal hormone production resume.
  • In one study, about 30% of people with formerly frozen ovarian tissue who had the thawed tissue reintroduced into the pelvis were able to give birth.
  • The first transplantation procedure of previously frozen ovarian tissue took place in 1999. Ovarian tissue freezing for fertility preservation was considered experimental until 2019.

Why would I choose to freeze my ovarian tissue or my child’s?

Women and girls facing certain medical treatment such as cancer surgery, radiation or chemotherapy, may experience impaired fertility or hormone production as a result. Individuals undergoing gender affirmation surgery may also wish to preserve ovarian tissue.

Ovarian tissue cryopreservation can remove the egg-producing portion of the ovary, called the ovarian cortex, before a patient’s treatment, freeze it, and store it. The tissue can be transplanted years later to make pregnancy possible.

In addition to increasing the possibility of pregnancy, ovarian tissue preservation also has the added benefit of hormone production. Damaged ovaries often cannot make important hormones such as estrogen or progesterone, requiring the woman to seek hormone therapy. Previously frozen, healthy ovarian tissue can be reimplanted after treatment to restore natural hormone production.

Ovarian Cryopreservation for Fertility Preservation: What Happens

When the patient is asleep under anesthesia, the surgeon makes a small incision (cut) in the abdomen and inserts a narrow tube called a laparoscope. Next, the surgeon removes tissue from the surface of one ovary, which contains immature eggs. Often, one ovary is removed to harvest an adequate amount of tissue. The tissue is carefully divided into small pieces, and flash frozen. Frozen ovarian tissue can be kept for several years in specially equipped storage facilities where samples are carefully monitored and preserved.

Once medical treatment is complete and the person who had ovarian tissue removed and frozen is ready to have ovaries restored, doctors can thaw the tissue and return it to the body. This procedure also uses a laparoscope. The surgeon may form a pocket in the peritoneum, a membrane that surrounds the organs in the abdomen, and deposit the tissue into the pelvis close to the remaining ovarian tissue.

After the thawed tissue is replaced, ovarian activity should begin or resume after a few months. At this point, pregnancy attempts can start under the guidance of a fertility specialist.

Pregnancy After Frozen Ovarian Tissue Transplantation

If the transplantation is successful and the patient is producing eggs, pregnancy can occur naturally or through in vitro fertilization (IVF). The procedure results in successful pregnancy in about one-third of patients, according to one study.

Risks of Freezing Ovarian Tissue

Harvesting the tissue and transplanting it are both surgical procedures that are performed with anesthesia, which involves risks. For patients with a blood cancer, such as leukemia, there is a risk of reintroducing cancerous cells into the body when the thawed ovarian tissue is reimplanted.

How much does ovarian tissue freezing cost?

There is a charge for the tissue-harvesting procedure, freezing and storage, as well as for the transplantation procedure when the person wishes to become pregnant. Insurance plans and some cancer support organizations may provide coverage for some or all of the costs involved in ovary tissue freezing.

The Johns Hopkins Fertility Preservation Innovation Center

Couple speaking with a doctor

The experts at the Johns Hopkins Fertility Preservation Innovation Center specialize in the latest techniques of fertility preservation, including egg, sperm and ovarian tissue freezing.

Request an Appointment

Find a Doctor
Find a Doctor