Center for Proteomics Discovery
Sample submission
Please submit samples through the iLab site of the Center for Proteomics Discovery.
Questions and consultations
Most questions about our services can be provided by our CPD AI consultant. The consultant program also reads input files (experimental plan, SDS-PAGE images, WB images, etc.) about a proposed project.
For additional consultation or questions, please contact [email protected].
Services
We assist investigators in identifying and quantifying proteins and their modifications that are differentially expressed in cells, tissues or body fluids, to track interactions with binding partners during changes in signal transduction, and to identify proteolytic cleavage sites and map post-translational modifications by providing the following proteomic services.
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Pre- and post-analysis
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- Sample preparation: Buffer exchange; column chromatography; proteolytic digestion; stable isotope labeling
- Protein identification: Proteins in solution, in gel bands or spots, or in complex protein extracts
Protein modifications: A growing list of known and custom post translational modifications (PTMs) which include acetylation, AMPylation, ADP ribosylation, citrullination, glycosylation, phosphorylation, proline hydroxylation, nitrosation, ubiquitination and novel cleavage sites, etc. - Protein quantification:
Untargeted quantification: Multi-dimensional protein identification technology (MuDPIT), isobaric mass tags (TMTs) or stable isotopic amino acids (SILAC)
Targeted quantification: parallel (PRM) reaction monitoring, internal standard triggered-parallel reaction monitoring (IS-PRM), and protein concentration ("absolute quantification") using standard peptides (AQUA) or recombinant proteins (PSAQ) - High resolution mass analysis: Accurate mass of intact proteins; characterization protein modifications
- Single-cell proteome analysis: We can analyze single cells from cell culture or tissues.
- Cell-type-specific proteome analysis: We can conduct cell-type-specific proteome analysis of tissues using antibody-mediated biotinylation (iCAB).
- Spatial proteomics: We can conduct spatial analysis of tissue sections by combining laser capture microdissection and LC-MS/MS.
- Monoclonal antibody sequencing: We can conduct de novo sequencing of a monoclonal antibody.
- Thermal proteome profiling for drug-binding protein identification: We can identify drug-binding proteins by measuring changes in protein stability when proteins bind a drug.
- Single-molecule analysis using Quantum-Si: We have a walk-up instrument for protein sequencing, which can detect several tens of proteins from ~100 ng of protein sample.
- Advanced statistical/bioinformatics analysis: PCA analysis, volcano plots, ingenuity-based gene ontology pathway analysis; bioinformatics consultation.
- Machine learning analysis for biomarker discovery
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- Sample preparation: Preparation of metabolomics/lipidomics samples
- Identification and quantification of metabolome/lipidome: Untargeted or targeted identification and quantification
- Advanced statistical/bioinformatics analysis
- Machine learning analysis for biomarker discovery
Our Team
Director: Chan-Hyun Na, Ph.D.
Technical Director/Proteomics specialist: Bob O’Meally, M.S.
Lab Manager: Jodie Franklin
Research Associate: Josh Smith, Ph.D.
Proteomics Specialist: David Kirchner, M.S.
Undergraduate Research Participant: Jessica Alius
Address:
WBSB 512
725 N. Wolfe St., Baltimore, MD 21205
Voice: 410-614-6968