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November 2019
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Molecular Biology and Genetics Archives

1. Breslow DK, Holland AJ . Mechanism and regulation of centriole and cilium biogenesis. Annu Rev Biochem. 2019 Jun 20;88:691-724. doi: 10.1146/annurev-biochem-013118-111153. Epub 2019 Jan 11.
Key words: cell cycle; centriole; centrosome; cilia; ciliopathy; mitosis
https://www.ncbi.nlm.nih.gov/pubmed/30601682

2. Cable J, Brangwynne C, Seydoux G, Cowburn D, Pappu RV, Castañeda CA, Berchowitz LE, Chen Z, Jonikas M, Dernburg A, Mittag T, Fawzi NL. Phase separation in biology and disease-a symposium report. Ann N Y Acad Sci. 2019 Jun 14. doi: 10.1111/nyas.14126. [Epub ahead of print]
Key words: biomolecular condensates; granules; membraneless organelles; phase diagram; phase separation; protein disorder
https://www.ncbi.nlm.nih.gov/pubmed/31199001

3. Cho C, Wang Y, Smallwood PM, Williams J, Nathans J. Molecular determinants in Frizzled, Reck, and Wnt7a for ligand-specific signaling in neurovascular development. Elife. 2019 Jun 21;8. pii: e47300. doi: 10.7554/eLife.47300.
Key words: CNS angiogenesis; Gpr124; Reck; Wnt7; blood-brain barrier; canonical Wnt signaling; developmental biology; mouse; neuroscience
https://www.ncbi.nlm.nih.gov/pubmed/31225798

4. Muszkieta L, Fontaine T, Beau R, Mouyna I, Vogt MS, Trow J, Cormack BP, Essen LO, Jouvion G, Latgé JP. The glycosylphosphatidylinositol-anchored DFG family is essential for the insertion of galactomannan into the ?-(1,3)-glucan-chitin core of the cell wall of Aspergillus fumigatus. mSphere. 2019 Jul 31;4(4). pii: e00397-19. doi: 10.1128/mSphere.00397-19.
Key words: Aspergillus fumigatus ; cell wall; glycobiology
https://www.ncbi.nlm.nih.gov/pubmed/31366710

5. Peng X, Williams J, Smallwood PM, Nathans J. Defining the binding interface of amyloid precursor protein (APP) and contactin3 (CNTN3) by site-directed mutagenesis. PLoS One. 2019 Jul 18;14(7):e0219384. doi: 10.1371/journal.pone.0219384. eCollection 2019.
Key words: amyloid precursor protein, contactin3, mutagenesis
https://www.ncbi.nlm.nih.gov/pubmed/31318883

1. Cho C, Wang Y, Smallwood PM, Williams J, Nathans J. Dlg1 activates beta-catenin signaling to regulate retinal angiogenesis and the blood-retina and bloodbrain barriers. Elife. 2019 May 8;8. pii: e45542. doi: 10.7554/eLife.45542.
Key words: blood-brain barrier; blood-retina barrier; canonical Wnt signaling; developmental biology; genetics; genomics; mouse; mouse genetics; retinal angiogenesis; vascular endothelial cells
http://www.ncbi.nlm.nih.gov/pubmed/31066677

2. DeFelice MM, Clark HR, Hughey JJ, Maayan I, Kudo T, Gutschow MV, Covert MW, Regot S. NF-?B signaling dynamics is controlled by a dose-sensing autoregulatory loop. Sci Signal. 2019 Apr 30;12(579). pii: eaau3568. doi: 10.1126/scisignal.aau3568.
Key words: NF-?B, signaling dynamics
http://www.ncbi.nlm.nih.gov/pubmed/31040261

3. Heng JS, Rattner A, Stein-O'Brien GL, Winer BL, Jones BW, Vernon HJ, Goff LA, Nathans J. Hypoxia tolerance in the Norrin-deficient retina and the chronically hypoxic brain studied at single-cell resolution. Proc Natl Acad Sci U S A. 2019 Apr 30;116(18):9103-9114. doi: 10.1073/pnas.1821122116. Epub 2019 Apr 15.
Key words: Norrie disease; familial exudative vitreoretinopathy; metabolomics; serine synthesis; singlecell RNA-seq
http://www.ncbi.nlm.nih.gov/pubmed/30988181

4. Kearse MG, Goldman DH, Choi J, Nwaezeapu C, Liang D, Green KM, Goldstrohm AC, Todd PK, Green R, Wilusz JE. Ribosome queuing enables non-AUG translation to be resistant to multiple protein synthesis inhibitors. Genes Dev. 2019 Jun 6. doi: 10.1101/gad.324715.119. [Epub ahead of print]
Key words: RAN translation; cycloheximide; near-cognate; start codon; translation initiation; translational control
http://www.ncbi.nlm.nih.gov/pubmed/31171704

5. LoMastro GM, Holland AJ. The emerging link between centrosome aberrations and metastasis. Dev Cell. 2019 May 6;49(3):325-331. doi: 10.1016/j.devcel.2019.04.002. Review.
Key words: aneuploidy; cancer; centrosomes; metastasis
http://www.ncbi.nlm.nih.gov/pubmed/31063752

6. Moyer TC, Holland AJ. PLK4 promotes centriole duplication by phosphorylating STIL to link the procentriole cartwheel to the microtubule wall. Elife. 2019 May 22;8. pii: e46054. doi: 10.7554/eLife.46054. [Epub ahead of print]
Key words: cell biology; human
http://www.ncbi.nlm.nih.gov/pubmed/31115335

7. Valotteau C, Prystopiuk V, Cormack BP, Dufrêne YF. Atomic force microscopy demonstrates that candida glabrata uses three Epa proteins to mediate adhesion to abiotic surfaces. mSphere. 2019 May 1;4(3). pii: e00277-19. doi: 10.1128/mSphere.00277-19.
Key words: AFM; Candida glabrata ; EPA ; adhesion
http://www.ncbi.nlm.nih.gov/pubmed/31043520

8. Wang Y, Sabbagh MF, Gu X, Rattner A, Williams J, Nathans J. Beta-catenin signaling regulates barrier-specific gene expression in circumventricular organ and ocular vasculatures. Elife. 2019 Apr 1;8. pii: e43257. doi: 10.7554/eLife.43257.
Key words: Wnt; beta-catenin; blood-brain barrier; circumventricular organ; developmental biology; endothelial; mouse; neuroscience; vascular
http://www.ncbi.nlm.nih.gov/pubmed/30932813

9. Wang Y, Liu S, Sun L, Xu N, Shan S, Wu F, Liang X, Huang Y, Luk E, Wu C, Zhou Z. Structural insights into histone chaperone Chz1-mediated H2A.Z recognition and histone replacement. PLoS Biol. 2019 May 20;17(5):e3000277. doi: 10.1371/journal.pbio.3000277. eCollection 2019 May.
Key words: histone, Chz1
http://www.ncbi.nlm.nih.gov/pubmed/31107867

1. Bowler M, Kong D, Sun S, Nanjundappa R, Evans L, Farner V, Holland A, Mahjoub MR, Sui H, Loncarek J. Highresolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy. Nat Commun. 2019 Mar 1;10(1):993.
Key words: centrioles, CCDC, appendage protein, DAs, SDAs
https://www.ncbi.nlm.nih.gov/pubmed/30824690

2. Kreutzberger AJB, Ji M, Aaron J, Mihaljevic L, Urban S. Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion. Science. 2019 Feb 1;363(6426). pii: eaao0076.
Key words: Rhomboid, membrane viscosity, enzyme diffusion, substrate molecules, Drosophila
https://www.ncbi.nlm.nih.gov/pubmed/30705155

3. Lee CY, Seydoux G. Dynamics of mRNA entry into stress granules. Nat Cell Biol. 2019;10.1038/s41556-019-0278-5. Key Words: Stress granules, SGs, mRNAs, Ras-GAP SH3 PMID: 30664788 Folkmann AW, Seydoux G. Spatial regulation of the polarity kinase PAR-1 by parallel inhibitory mechanisms. Development. 2019 Mar 25;146(6). pii: dev171116.
Key words: Kinase, MEX-6, P granules, PAR proteins, PAR-3, Polarity

https://www.ncbi.nlm.nih.gov/pubmed/30814118

4. Mohammad F, Green R, Buskirk AR. A systematically-revised ribosome profiling method for bacteria reveals pauses at single-codon resolution. Elife. 2019 Feb 6;8. pii: e42591.
Key words: E. coli, bacteria, biochemistry, chemical biology, ribosome profiling
https://www.ncbi.nlm.nih.gov/pubmed/30724162

5. Putnam A, Cassani M, Smith J, Seydoux G. A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos. Nat Struct Mol Biol. 2019 Mar;26(3):220-226.
Key words: RNA granules, Caenorhabditis, elegans, embryos
https://www.ncbi.nlm.nih.gov/pubmed/30833787

6. Wu CC, Zinshteyn B, Wehner KA, Green R. High-Resolution ribosome profiling defines discrete ribosome elongation states and translational regulation during cellular stress. Mol Cell. 2019 Mar 7;73(5):959-970.e5.
Key words: eEF2 phosphorylation, ribosome functional states, ribosome profiling, translation elongation
https://www.ncbi.nlm.nih.gov/pubmed/30686592

1. Bowler M, Kong D, Sun S, Nanjundappa R, Evans L, Farner V, Holland A, Mahjoub MR, Sui H, Loncarek J. Highresolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy. Nat Commun. 2019 Mar 1;10(1):993.
Key words: centrioles, CCDC, appendage protein, DAs, SDAs
https://www.ncbi.nlm.nih.gov/pubmed/30824690

2. Breslow DK, Holland AJ. Mechanism and regulation of centriole and cilium biogenesis. Annu Rev Biochem. 2019 Jan;10.1146/annurev-biochem-013118-111153. [Epub ahead of print].
Key words: Centriole, centrosome, cilia, ciliopathy, cell cycle, mitosis
https://www.ncbi.nlm.nih.gov/pubmed/30601682

3. Corden JL. An arginine nexus in the RNA Polymerase II CTD. Mol Cell. 2019 Jan 3;73(1):3-4.
Key words: C-Termination domain, CTD, RNA polymerase II, arginine deiminase, citrulline
https://www.ncbi.nlm.nih.gov/pubmed/30609390

4. Folkmann AW, Seydoux G. Spatial regulation of the polarity kinase PAR-1 by parallel inhibitory mechanisms. Development. 2019 Mar 25;146(6). pii: dev171116. [Epub ahead of print].
Key words: Kinase, MEX-6, P granules, PAR proteins, PAR-3, Polarity
https://www.ncbi.nlm.nih.gov/pubmed/30814118

5. Kreutzberger AJB, Ji M, Aaron J, Mihaljevic L, Urban S. Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion. Science. 2019 Feb 1;363(6426).
Key words: Rhomboid, membrane viscosity, enzyme diffusion, substrate molecules, Drosophila
https://www.ncbi.nlm.nih.gov/pubmed/30705155

6. Lee CY, Seydoux G. Dynamics of mRNA entry into stress granules. Nat Cell Biol. 2019 Feb;21(2):116-117.
Key words: Stress granules, SGs, mRNAs, Ras-GAP SH3
https://www.ncbi.nlm.nih.gov/pubmed/30664788

7. Mohammad F, Green R, Buskirk AR. A systematically-revised ribosome profiling method for bacteria reveals pauses at single-codon resolution. Elife. Feb 6;8. pii: e42591.
Key words: E. coli, bacteria, biochemistry, chemical biology, ribosome profiling
https://www.ncbi.nlm.nih.gov/pubmed/30724162

8. Putnam A, Cassani M, Smith J, Seydoux G. A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos. Nat Struct Mol Biol. 2019 Mar;26(3):220-226.
Key words: RNA granules, Caenorhabditis, elegans, embryos https://www.ncbi.nlm.nih.gov/pubmed/30833787

9. Wu CC, Zinshteyn B, Wehner KA, Green R. High-Resolution ribosome profiling defines discrete ribosome elongation states and translational regulation during cellular stress. Mol Cell. Mar 7;73(5):959-970.e5.
Key words: eEF2 phosphorylation, ribosome functional states, ribosome profiling, translation elongation
https://www.ncbi.nlm.nih.gov/pubmed/30686592

10. Zinshteyn B, Chan D, England W, Feng C, Green R, Spitale RC. Assaying RNA structure with LASER-Seq. Nucleic Acids Res. 2019 Jan 10;47(1):43-55.
Key words: RNA molecules, LASER, SHAPE, Watson-Crick, LASER-seq https://www.ncbi.nlm.nih.gov/pubmed/30476193

1. Wang Y, Cho C, Williams J, Smallwood PM, Zhang C, Junge HJ, Nathans J. Interplay of the Norrin and Wnt7a/Wnt7b signaling systems in blood-brain barrier and blood-retina barrier development and maintenance. Proc Natl Acad Sci USA. 2018 Dec 11;115(50):E11827-E11836.
Key words: Central nervous system, mouse genetics, vascular endothelial cells, ?-catenin signaling
https://www.ncbi.nlm.nih.gov/pubmed/30478038

2. Zinshteyn B, Chan D, England W, Feng C, Green R, Spitale RC. Assaying RNA structure with LASER-Seq. Nucleic Acids Res. 2018 Nov 22. doi: 10.1093/nar/gky1172. [Epub ahead of print]
Key words: RNA-protein complexes, LASER-Seq, LASER, C8 position, RT-stop, mutational profiling (MaP)
https://www.ncbi.nlm.nih.gov/pubmed/30476193

1. Folkmann AW, Seydoux G. Single-molecule study reveals the frenetic lives of proteins in gradients. Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9336-9338.
Key words: Caenorhabditis elegans zygotes, Drosophila embryos, Bicoid protein
https://www.ncbi.nlm.nih.gov/pubmed/30181287

2. Forman-Kay JD, Kriwacki RW, Seydoux G. Phase separation in biology and disease. J Mol Biol. 2018 Nov 2;430(23):4603-4606.
Key words: Cytoplasm, star fish, membrane-less organelles, RGG/RG motif-containing proteins
https://www.ncbi.nlm.nih.gov/pubmed/30213727

3. Kreutzberger AJB, Urban S. Single-molecule analyses reveal rhomboid proteins are strict and functional monomers in the membrane. Biophys J. 2018 Oct 2. pii: S0006-3495(18)31105-6.
Key words: Rhomboid Proteins; hydrolyze peptide; intramembrane proteases; photobleaching; monomeric; oligomeric
https://www.ncbi.nlm.nih.gov/pubmed/30342748

4. Leda M, Holland AJ, Goryachev AB. Autoamplification and competition drive symmetry breaking: initiation of centriole duplication by the PLK4-STIL network. iScience. 2018 Oct 26;8:222-235.
Key words: Biological Sciences; Developmental Biology; In Silico Biology
https://www.ncbi.nlm.nih.gov/pubmed/30340068

5. Luo J, Sun X, Cormack BP, Boeke. Karyotype engineering by chromosome fusion leads to reproductive isolation in yeast. 2018 Aug;560(7718):392-396.
Key words: karyotype, CRISPER-Cas9-mediated, yeast sporulation
https://www.ncbi.nlm.nih.gov/pubmed/30069047

6. Peng X, Emiliani F, Smallwood PM, Rattner A, Lei H, Sabbagh MF, Nathans J. Affinity capture of polyribosomes followed by RNAseq (ACAPseq), a discovery platform for proteinprotein interactions. Elife. 2018 Oct 22;7. pii: e40982. doi: 10.7554/eLife.40982.
Key words: RNAseq; biochemistry; chemical biology; extracellular interactome; protocadherin
https://www.ncbi.nlm.nih.gov/pubmed/30345971

7. Sabbagh MF, Heng JS, Luo C, Castanon RG, Nery JR, Rattner A, Goff LA, Ecker JR, Nathans J. Transcriptional and epigenomic landscapes of CNS and non-CNS vascular endothelial cells. eLife. 2018 Sep 6;7. pii: e36187.
Key words: DNA methylation, Wnt, accessible chromatin, blood-brain barrier, vascular endothelial cell
https://www.ncbi.nlm.nih.gov/pubmed/30188322

8. Seydoux G. The P Granules of C. elegans: A genetic model for the study of RNA-protein condensates. J Mol Biol. 2018 Nov 2;430(23):4702-4710.
Key words: C. elegans, Germline condensates, P granules, RNA granules
https://www.ncbi.nlm.nih.gov/pubmed/30096346

9. Shaytan AK, Xiao H, Armeev GA, Gaykalova DA, Komarova GA, Wu C Studitsky VM, Landsman D, Panchenko AR. Structural interpretation of DNA-protein hydroxyl-radical footprinting experiments with high resolution using HYDROID. Nat Protoc. 2018 Nov;13(11):2535-2556.
Key words: HYDROID; hydroxyl-radical footprinting (HRF); DNAse I; DNA cleavage frequency
https://www.ncbi.nlm.nih.gov/pubmed/30341436

1. Levine MS, Holland AJ. The impact of mitotic errors on cell proliferation and tumorigenesis. Genes Dev. 2018 May 1;32(9-10):620-638.
Key words: aneuploidy, chromosomal instability, mitosis
https://www.ncbi.nlm.nih.gov/pubmed/29802124

1. Alder JK, Hanumanthu VS, Strong MA, DeZem AE, Stanley SE, Takemoto CM, Danilova L, Applegate CD, Bolton SG, Mohr DW, Brodsky RA, Casella JF, Greider CW, Jackson JB, Armanios M. Diagnostic utility of telomere length testing in a hospital-based setting. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):E2358-E2365.
Key words: aplastic anemia; interstitial lung disease; liver disease; precision medicine
https://www.ncbi.nlm.nih.gov/pubmed/29463756

2. Heerma van Voss MR, Kammers K, Vesuna F, Brilliant J, Bergman Y, Tantravedi S, Wu X, Cole RN, Holland A, van Diest PJ, Raman V. Global effects of DDX3 inhibition on cell cycle regulation identified by a combined phosphoproteomics and single cell tracking approach. Transl Oncol. 2018 Jun;11(3):755-763.
Key words: DDX3, RK-33, DEAD box RNA, ATPase dependent
https://www.ncbi.nlm.nih.gov/pubmed/29684792

3. Schuller AP, Green R. Roadblocks and resolutions in eukaryotic translation. Nat Rev Mol Cell Biol. 2018 May 14. doi: 10.1038/s41580-018-0011-4. [Epub ahead of print]
Key words: aminoacyl-tRNAs, ribosome rescue, hypusine
https://www.ncbi.nlm.nih.gov/pubmed/29760421

4. Ward A, Kumari G, Sen R, Desiderio S. The RAG-2 inhibitory domain gates accessibility of the V(D)J recombinase to chromatin. Mol Cell Biol. 2018 May 14. pii: MCB.00159-18.
Key words: Gating of chromatin, RAG-2, of H3K4me3, spontaneous V(D)J
https://www.ncbi.nlm.nih.gov/pubmed/29760281

1. Dever TE, Dinman JD, Green R. Translation elongation and recoding in eukaryotes. Cold Spring Harb Perspect Biol. 2018 April 2;10.1101/xahpweapwxr.032649 [Epub ahead of print]
Key words: eIF5A eukaryotes ribosomal frameshifting elongation
https://www.ncbi.nlm.nih.gov/pubmed/29610120

2. Evans LT, Holland AJ. Pushed out of a tough crowd: centrosome aberrations promote invasiveness. EMBO J. 2018 April 5;10.15252/embj.201899422. [Epub ahead of print]
Key words: Centrosome non-cell-autonomous aberrations
https://www.ncbi.nlm.nih.gov/pubmed/29622546

3. Liakath-Ali K, Mills EW, Sequeira I, Lichtenberger BM, Pisco AO, Sipila KH, Mishra A, Yoshikawa H, Wu CC, Ly T, Lamond Al, Adham IM, Green R, Watt FM. An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis. Nature. 2018 April; 556(7701):376-380.
Key words: mRNA epidermal homeostasis evolutionarily ribosome-rescue
https://www.ncbi.nlm.nih.gov/pubmed/29643507

4. Nigg EA, Holland AJ. Once and only once: mechanisms of centriole duplication and their deregulation disease. Nat Rev Mol Cell Biol. 2018 May; 19(5):297-312.
Key words: Centrioles cilia flagella organelles
https://www.ncbi.nlm.nih.gov/pubmed/29363672

1. Kudo T, Jeknic S, Macklin DN, Akhter S, Hughey JJ, Regot S, Covert MW. Live-cell measurements of kinase activity in single cells using translocation reporters. Nat Protoc. 2018 Jan. 13(1):155-169. Doi: 10.1038/nprot.2017.128. Epub 2017 Dec 21
Key words: Image processing, kinases, protein translocation
https://www.ncbi.nlm.nih.gov/pubmed/29266096

2. Schuller AP, Zinshteyn B, Enam SU, Green R. Directed hydroxyl radical probing reveals Upf1 binding to the 80S ribosomal E site rRNA at the L1 stalk. Nucleic Acids Res. 2017 Dec. doi 10.1093/nar/gkx1263. [Epub ahead of print]
Key words: Upf1 RNA protein complexes ribosome
https://www.ncbi.nlm.nih.gov/pubmed/29253221

1. Besold AN, Gilston BA, Radin JN, Ramsoomair C, Culbertson EM, Li CX, Cormack BP, Chazin WJ, Kehl-Fie TE, Culotta VC. The role of calprotectin in withholding zinc and copper from Candida albicans. Infect Immun. 2017 Nov 13. pii: IAI.00779-17. doi: 10.1128/IAI.00779-17. [Epub ahead of print]
Key words: calprotectin, zinc, copper, Candida albicans
https://www.ncbi.nlm.nih.gov/pubmed/29133349

2. Cook JD, Ng SY, Lloyd M, Eddington S, Sun H, Nathans J, Bok D, Radu RA, Travis GH. Peropsin modulates transit of vitamin A from retina to retinal pigment epithelium. J Biol Chem. 2017 Nov 6. pii: jbc.M117.812701. doi: 10.1074/jbc.M117.812701. [Epub ahead of print]
Key words: G protein-coupled receptor (GPCR); gene knockout; membrane; photoreceptor; retina; retinal metabolism; retinoid-binding protein; retinol; vitamin A
https://www.ncbi.nlm.nih.gov/pubmed/29109151

3. Lee CS, Lu T, Seydoux G. Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B. Elife. 2017 Nov 7;6. pii: e30201. doi: 10.7554/eLife.30201. [Epub ahead of print].
Key words: C. elegans; cell biology; developmental biology; stem cells
https://www.ncbi.nlm.nih.gov/pubmed/29111977

4. Levine M, Holland A. Cell cycle proteins moonlight in multiciliogenesis. Science. 2017 Nov 10;358(6364):716-718. doi: 10.1126/science.aaq0770.
Key words: cell cycle, proteins, multiciliogenesis
https://www.ncbi.nlm.nih.gov/pubmed/29123048

5. Lu W, Dordai D, Huso D, Desiderio S. Smoothened signaling in the mouse osteoblastoid lineage is required for efficient B lymphopoiesis. Blood. 2017 Nov 22. pii: blood-2017-06-793539. doi: 10.1182/blood-2017-06-793539. [Epub ahead of print]
Key words: mouse, osteoblastoid, B lymphopoiesis
https://www.ncbi.nlm.nih.gov/pubmed/29167177

6. Mills EW, Green R. Ribosomopathies: There's strength in numbers. Science. 2017 Nov 3;358(6363). pii: eaan2755. doi: 10.1126/science.aan2755.
Key words: ribosomopathies, ribosomal protein haploinsufficiency, homeostasis, Diamond-Blackfan anemia
https://www.ncbi.nlm.nih.gov/pubmed/29097519

7. Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G. Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks. Proc Natl Acad Sci U S A. 2017 Dec 12;114(50):E10745-E10754. doi: 10.1073/pnas.1711979114.
Key words: CRISPR; HDR; PCR repair template; SDSA; short homology arms
https://www.ncbi.nlm.nih.gov/pubmed/29183983

8. Rossi DCP, Gleason JE, Sanchez H, Schatzman SS, Culbertson EM, Johnson CJ, McNees CA, Coelho C, Nett JE, Andes DR, Cormack BP, Culotta VC. Candida albicans FRE8 encodes a member of the NADPH oxidase family that produces a burst of ROS during fungal morphogenesis. PLoS Pathog. 2017 Dec 1;13(12):e1006763. doi: 10.1371/journal.ppat.1006763. eCollection 2017 Dec.
Key words: Candida albicans, NADPH oxidase, fungal morphogenesis
https://www.ncbi.nlm.nih.gov/pubmed/29194441

9. Xiao H, Wang F, Wisniewski J, Shaytan AK, Ghirlando R, FitzGerald PC, Huang Y, Wei D, Li S, Landsman D, Panchenko AR, Wu C. Genes Dev. Molecular basis of CENP-C association with the CENP-A nucleosome at yeast centromeres. 2017 Oct 1;31(19):1958-1972. doi: 10.1101/gad.304782.117. Epub 2017 Oct 26.
Key words: AT-rich DNA; Cse4/CENP-A; Mif2/CENP-C; budding yeast; centromere; nucleosome
https://www.ncbi.nlm.nih.gov/pubmed/29074736

10. Yoon KJ, Ringeling FR, Vissers C, Jacob F, Pokrass M, Jimenez-Cyrus D, Su Y, Kim NS, Zhu Y, Zheng L, Kim S, Wang X, Doré LC, Jin P, Regot S, Zhuang X, Canzar S, He C, Ming GL, Song H. Temporal control of mammalian cortical neurogenesis by m6A methylation. Cell. 2017 Nov 2;171(4):877-889.e17. doi: 10.1016/j.cell.2017.09.003. Epub 2017 Sep 28.
Key words: Mettl14; RNA methylation; autism; epitranscriptomics; human organoid; m(6)A; neurogenesis; radial glia cell; schizophrenia; transcriptional prepatterning
https://www.ncbi.nlm.nih.gov/pubmed/28965759

1. Guydosh NR, Kimmig P, Walter P, Green R. Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe. Elife. 2017 Sep 25;6. pii: e29216. doi: 10.7554/eLife.29216. [Epub ahead of print].
Key words: S. pombe; cell biology
https://www.ncbi.nlm.nih.gov/pubmed/28945192

2. Mok CA, Au V, Thompson OA, Edgley ML, Gevirtzman L, Yochem J, Lowry J, Memar N, Wallenfang MR, Rasoloson D, Bowerman B, Schnabel R, Seydoux G, Moerman DG, Waterston RH. MIP-MAP: High-throughput mapping of Caenorhabditis elegans temperature-sensitive mutants via molecular inversion probes. Genetics. 2017 Oct;207(2):447-463. doi: 10.1534/genetics.117.300179. Epub 2017 Aug 21.
Key words: Caenorhabditis elegans; genetic mapping; massively multiplex sequencing; molecular inversion probes; temperature-sensitive mutations
https://www.ncbi.nlm.nih.gov/pubmed/28827289

3. Tischfield MA, Robson CD, Gilette NM, Chim SM, Sofela FA, DeLisle MM, Gelber A, Barry BJ, MacKinnon S, Dagi LR, Nathans J, Engle EC. Cerebral vein malformations result from loss of Twist1 expression and BMP signaling from skull progenitor cells and dura. Dev Cell. 2017 Sep 11;42(5):445-461.e5. doi: 10.1016/j.devcel.2017.07.027. Epub 2017 Aug 30.
Key words: BMP2; BMP4; ICP; Twist1; cerebral veins; coronal suture; craniosynostosis; osteoblast; skull; venous angiogenesis
https://www.ncbi.nlm.nih.gov/pubmed/28844842

4. Wang S, Pike AM, Lee SS, Strong MA, Connelly CJ, Greider CW. BRD4 inhibitors block telomere elongation. Nucleic Acids Research. 2017 Aug 21;45(14):8403-8410.
Key words: phosphotransferases telomerase telomere mice telomere shortening
https://www.ncbi.nlm.nih.gov/pubmed/28854735

1. Breitkopf-Heinlein K, Meyer C, König C, Gaitantzi H, Addante A, Thomas M, Wiercinska E, Cai C, Li Q, Wan F, Hellerbrand C, Valous NA, Hahnel M, Ehlting C, Bode JG, Müller-Bohl S, Klingmüller U, Altenöder J, Ilkavets I, Goumans MJ, Hawinkels LJ, Lee SJ, Wieland M, Mogler C, Ebert MP, Herrera B, Augustin H, Sánchez A, Dooley S, Ten Dijke P. BMP-9 interferes with liver regeneration and promotes liver fibrosis. Gut. 2017 May;66(5):939-954.
Key words: cytokines; growth factors; hepatic fibrosis; hepatic stellate cell; hepatocyte
https://www.ncbi.nlm.nih.gov/pubmed/28336518

2. Guydosh NR, Green R. Translation of poly(A) tails leads to precise mRNA cleavage.
RNA. 2017 May;23(5):749-761.
Key words: Hbs1; PELO; cryptic polyadenylation; ribosome stalling; ski complex
https://www.ncbi.nlm.nih.gov/pubmed/28193672

3. Lambrus BG, Holland AJ. A New Mode of Mitotic Surveillance. Trends Cell Biol. 2017 May;27(5):314-321.
Key words: cell cycle arrest; centrosome; checkpoint; mitosis; surveillance
https://www.ncbi.nlm.nih.gov/pubmed/28188027

4. McClary B, Zinshteyn B, Meyer M, Jouanneau M, Pellegrino S, Yusupova G, Schuller A, Reyes JCP, Lu J, Guo Z, Ayinde S, Luo C, Dang Y, Romo D, Yusupov M, Green R, Liu JO. Inhibition of eukaryotic translation by the antitumor natural product agelastatin A. Cell Chem Biol. 2017 May 18;24(5):605-613.e5.
Key words: agelastatin A; brain cancer; chemical footprinting; drug design; marine alkaloid; molecular docking; peptidyl transferase center; rRNA seq; ribosome; translation elongation
https://www.ncbi.nlm.nih.gov/pubmed/28457705

1. Buskirk AR, Green R. Ribosome pausing, arrest and rescue in bacteria and eukaryotes. Philos Trans R Soc Lond B Biol Sci. 2017 Mar 19;372(1716).
Key words: Dom34/PELO, EFP, Hbs1/HBS1I; Rli1ABCE1, elF5A
http://rstb.royalsocietypublishing.org/content/372/1716/20160183
PMID: 28138069

1. Bettridge J, Na CH, Pandey A, Desiderio S. H3K4me3 induces allosteric conformational changes in the DNA-binding and catalytic regions of the V(D)J recombinase. Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):1904-1909. 10.1073/pnas.1615727114.
Key words: DNA recombination allosteric control epigenetic
http://www.pnas.org/content/114/8/1904
PMID: 28174273

2. Chang H, Smallwood PM, Williams J, Nathans J. Intramembrane proteolysis of astrotactins. J Biol Chem.
2017 Feb 24;292(8):3506-3516.
Key words: Intramembrane proteolysis membrane protein transmembrane domain
http://www.jbc.org/content/292/8/3506
PMID: 28100779

3. Dakhiya Y, Hussien D, Fridman E, Kiflawi M, Green R. Correlations between circadian rhythms and growth in challenging environments. Nat Commun. 2017 Mar;173(3):1724-1734.
Key words: Hypomorphic mutations, PolyA, translation, expression, elongation
http://www.nature.com/articles/ncomms14705
PMID: 28153924

4. Guydosh NR, Green R. Translation of poly(A) tails leads to precise mRNA cleavage. RNA
2017 Feb 13. pii: rna.060418.116.
Key words: Translation, poly(A) tails, mRNA decay, ribosome profiling
http://rnajournal.cshlp.org/content/early/2017/02/13/rna.060418.116
PMID: 28193672

5. Mills EW, Green R, Ingolia NT. Slowed decay of mRNAs enhances platelet specific translation. Blood.
2017 Feb 17. pii: blood-2016-08-736108.
Key words: Ribosome profiling, human platelets, megakaryocyte, mRNA decay, PELO
http://www.bloodjournal.org/content/early/2017/02/17/blood-2016-08-736108?sso-checked=true
PMID: 28213379

1. Arthur LL, Chung JJ, Jankirama P, Keefer KM, Kolotilin I, Pavlovic-Djuranovic S, Chalker DL, Grbic V, Green R, Menassa R, True HL, Skeath JB, Djuranovic S. Rapid generation of hypomorphic mutations. Nat Commun. 2017 Jan 20;8:14112.
Key words: Hypomorphic mutations polyA translation expression elongation
http://www.nature.com/ncomms/2017/170120/
ncomms14112/full/ncomms14112.html
PMID: 28106166

2. Baker RP, Urban S. An inducible reconstitution system for the real-time kinetic analysis of protease activity and inhibition inside the membrane. Methods Enzymol.
2017;584:229-253.
Key words: Alzheimer's disease cancer cell signaling ER-association gamma-secretase
http://dx.doi.org/10.1016/bs.mie.2016.10.025
PMID: 28065265

3. Chung HK, Ryu D, Kim KS, Chang JY, Kim YK, Yi HS, Kang SG, Choi MJ, Lee SE, Jung SB, Ryu MJ, Kim SJ, Kweon GR, Kim H, Hwang JH, Lee CH, Lee SJ, Wall CE, Downes M, Evans RM, Auwerx J, Shong M. Growth differentiation factor 15 is a myomitokine governing systemic energy homeostasis. J Cel Biol. 2017 Jan 2;216(1):149-165.
Key words: GoF-15 TGF-ß muscle metabolism
http://jcb.rupress.org/content/216/1/149
PMID: 27986797

4. Eleftheriou NM, Sjölund J, Bocci M, Cortez E, Lee SJ, Cunha SI, Pietras K. Compound genetically engineered mouse models of cancer reveal dual targeting of ALK1 and endoglin as a synergistic opportunity to impinge on angiogenic TGF-? signaling. Oncotarget. 2016 Dec 20;7(51):84314-84325.
Key words: "ALK1 bmp9 angiogenesis endoglin"
http://dx.doi.org/10.18632/oncotarget.12604
PMID: 27741515

5. Housden BE, Muhar M, Gemberling M, Gersbach CA, Stainier DY, Seydoux G. Loss-of-function genetic tools for animal models: cross-species and cross-platform differences. Nat Rev Genet.
2017 Jan;18(1):24-40.
Key words: Caenorhabditis elegans loss-of-function genetic engineering
http://www.nature.com/nrg/journal/v18/n1/full/nrg.2016.118.html
PMID: 27795562

6. Lambrus BG, Holland AJ. A new mode of mitotic surveillance. Trends Cell Biol. 2017 Feb 7. pii: S0962-8924(17)30014-4.
Key words: Centrosome mitosis cell cycle arrest
http://www.sciencedirect.com/science/article/pii/S0962892417300144
PMID: 28188027

7. Levine MS, Bakker B, Boeckx B, Moyett J, Lu J, Vitre B, Spierings DC, Lansdorp PM, Cleveland DW, Lambrechts D, Foijer F, Holland AJ. Centrosome Amplification is sufficient to promote spontaneous tumorigenesis in mammals. Dev Cell. 2017 Feb 6;40(3):313-322.e5.
Key words: Plk4 aneuploidy centriole centrosome amplification
http://www.sciencedirect.com/science/article/pii/S1534580716309170
PMID: 28132847

8. Muir AM, Massoudi D, Nguyen N, Keene DR, Lee SJ, Birk DE, Davidson JM, Marinkovich MP, Greenspan DS. BMP1-like proteinases are essential to the structure and wound healing of skin. Matrix Biol. 2016 Dec;56:114-131.
Key words: Biglycan bone morphogenetic protein mammalian
http://dx.doi.org/10.1016/j.matbio.2016.06.004
PMID: 27363389

9. Park BV, Freeman ZT, Ghasemzadeh A, Chattergoon MA, Rutebemberwa A, Steigner J, Winter ME, Huynh TV, Sebald SM, Lee SJ, Pan F, Pardoll DM, Cox AL. TGF?1-Mediated SMAD3 Enhances PD-1 Expression on Antigen-Specific T Cells in Cancer. Cancer Discov. 2016 Dec;6(12):1366-1381.
Key words: SMAD3 TGF-ß immune system
http://cancerdiscovery.aacrjournals.org/search/Mediated%
252BSMAD3%252BEnhances%252BPD-1

PMID: 27683557

10. Urban S. Snapshot: cartography of intramembrane proteolysis. Cell. ec 15;167(7):1898-1898.e1.
Key words: Intramembrane proteolysis membranes organelles regulation

http://www.sciencedirect.com/science/article/pii/S0092867416316531
PMID: 27984738

Smith J, Calidas D, Schmidt H, Lu T, Rasoloson D, Seydoux G. Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. eLife 2016;10.7554/eLife.21337.
Key words: RNA granule phase separation
https://elifesciences.org/content/5/e21337
PMID:27914198

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November 2019 articles:
Changes in HSR Recertification
Research Billing Buzz eNewsletter - November 2019
Update on Insurance and Research Participant Financial Responsibility Information Sheet: Signature No Longer Required
Change in JHM IRB Review Fees for Commercially-Funded Studies
Data and Safety Monitoring Board Training Manual Now Available
Good Clinical Practice (GCP) Fundamentals: Understanding and Applying GCP to Human Subject Research
Fee Schedule Adjustment for Hospital Services Charged to Clinical Trial Budgets
Changes to NIH-funded Human Subjects Research
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NIH Policy on Foreign Component Involvement
Why Project Outcomes Matter in your Interim and Final Research Performance Progress Report (RPPR)
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