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COVID-19 Update

Clinical Resources for Severe Alcoholic Hepatitis Investigation

Alcoholic hepatitis is frequently a severe clinical condition associated with high short-term mortality. Research in this field has been hampered by both the absence of an appropriate animal model and the lack of readily available human liver samples to conduct translational investigations in patients with severe alcoholic hepatitis.

In 2012 following the French report researchers at Johns Hopkins began a program for transplantation of patients with severe alcoholic hepatitis at Johns Hopkins with promising clinical outcomes. The program for transplantation of patients with severe alcoholic hepatitis also allowed for the collection of liver samples and data relevant to the study of severe alcoholic hepatitis with committed investigators to promote discovery in this field.

With R24 grant support from the National Institute on Alcohol Abuse and Alcoholism (NIAAA), our team established a centralized database of de-identified samples in order to promote access to otherwise unavailable specimens, collaboration, efficiency, and progress towards a cure. In collaboration with experts from the High Throughput Biology Center at Johns Hopkins, we also utilize this resource to perform transcriptome and proteome analysis and provide these data to committed investigators for translational research. In last three years, we have provided several hundreds human samples to 37 principal investigators from 26  institutions/universities. 

Samples may be provided to principal investigators following a thorough vetting by the research team. 

Severe Alcoholic Hepatitis Liver Samples

Severe Alcoholic Hepatitis Liver Sample
Unstained liver (donor)


Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)


Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (AH)


Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (donor)


Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)


Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)


Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (AH)


Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (donor)


Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (donor)


Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)


Flow cytometry (FC) analysis (FSC/SSC) of AH leukocytes and peripheral blood mononuclear cells.
Flow cytometry (FC) analysis (FSC/SSC)

Flow cytometry (FC) analysis (FSC/SSC) of AH leukocytes and peripheral blood mononuclear cells.

Flow cytometry (FC) analysis (single-color) of AH leukocytes and peripheral blood mononuclear cells.
Flow cytometry (FC) analysis (single-color)

Flow cytometry (FC) analysis (single-color) of AH leukocytes and peripheral blood mononuclear cells.

Immuno-fluorescent (IF) staining of AH leukocytes and peripheral blood mononuclear cells
Immuno-fluorescent staining

Immuno-fluorescent (IF) staining of AH leukocytes and peripheral blood mononuclear cells (cyto-spin slides).

Hematoxylin staining of AH peripheral blood mononuclear cells (cyto-spin slides).
Hematoxylin staining

Hematoxylin staining of AH peripheral blood mononuclear cells (cyto-spin slides).

Hematoxylin staining of AH leukocytes (cyto-spin slides).
Hematoxylin staining of AH leukocytes (cyto-spin slides)


Severe Alcoholic Hepatitis Liver Samples

Severe Alcoholic Hepatitis Liver Sample
Unstained liver (donor)
Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)
Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (AH)
Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (donor)
Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)
Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)
Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (AH)
Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (donor)
Severe Alcoholic Hepatitis Liver Sample
H&E-stained liver (donor)
Severe Alcoholic Hepatitis Liver Sample
IHC-stained liver (AH)
Flow cytometry (FC) analysis (FSC/SSC) of AH leukocytes and peripheral blood mononuclear cells.
Flow cytometry (FC) analysis (FSC/SSC) Flow cytometry (FC) analysis (FSC/SSC) of AH leukocytes and peripheral blood mononuclear cells.
Flow cytometry (FC) analysis (single-color) of AH leukocytes and peripheral blood mononuclear cells.
Flow cytometry (FC) analysis (single-color) Flow cytometry (FC) analysis (single-color) of AH leukocytes and peripheral blood mononuclear cells.
Immuno-fluorescent (IF) staining of AH leukocytes and peripheral blood mononuclear cells
Immuno-fluorescent staining Immuno-fluorescent (IF) staining of AH leukocytes and peripheral blood mononuclear cells (cyto-spin slides).
Hematoxylin staining of AH peripheral blood mononuclear cells (cyto-spin slides).
Hematoxylin staining Hematoxylin staining of AH peripheral blood mononuclear cells (cyto-spin slides).
Hematoxylin staining of AH leukocytes (cyto-spin slides).
Hematoxylin staining of AH leukocytes (cyto-spin slides)

Publicly available datasets generated under this grant:

RNAseq analysis highlights significant transcriptional changes within the livers of patients with alcoholic hepatitis.

NIH GEO - Accession GSE143318
 

Contact the Research Team

 

Other questions related to our resource, please contact Rebecca Prince 443-287-7789

 

Research Team

Principal Investigator

Photo of Dr. Zhaoli Sun, M.D., Ph.D.

Sun, Zhaoli, M.D., Ph.D.

Associate Professor of Surgery
 
 

Co-Investigators

Photo of Dr. Robert Albert Anders, M.D., Ph.D.

Anders, Robert Albert, M.D., Ph.D.

Associate Professor of Pathology
Assistant Professor of Oncology
 
Photo of Dr. Andrew MacGregor Cameron, M.D., Ph.D.

Cameron, Andrew MacGregor, M.D., Ph.D.

Professor of Surgery
Chief, Division of Transplantation
Surgical Director of Liver Transplantation
 
Photo of Dr. Jiang Qian, M.S., Ph.D.

Qian, Jiang, M.S., Ph.D.

Professor of Ophthalmology
Professor of Oncology
 
Photo of Dr. Heng Zhu, Ph.D.

Zhu, Heng, Ph.D.

Professor of Pharmacology and Molecular Sciences
Professor of Oncology
Co-Director, High Throughout Biology Center
 
   
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