Mycobacterium abscessus is a rapidly growing non-tuberculous mycobacterium that causes life-threatening opportunistic infections in individuals with structural lung conditions such as bronchiectasis, cystic fibrosis, and chronic obstructive pulmonary diseases. As no FDA-approved treatments exist to treat this disease, to support development of new and effective treatments, preclinical animal models are necessary. The aim of this proposal is to develop a mouse model of chronic M. abscessus lung disease that is simple and not resource-intensive to implement.