The overall goal of this research program is to study dynamics of the latent reservoir through single cell analysis of the viral decay processes that determine the composition of the reservoir. A fundamental hypothesis of this research program is that the latent reservoir is composed of cells that survive the rapid decay processes that eliminate the vast majority of infected cells. We believe that insights into reservoir dynamics can be obtained through analysis of these decay processes. We will analyze these decay processes in coordinated studies in PLWH (Drs. Robert and Janet Siliciano, Project 1) and in SIV-infected macaques (Dr. Dan Barouch, Project 2) with the help of Dr. Alan Perelson and the Modeling Core.