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Bo Young Cha, Ph.D

Research Associate


Phone number:  410-955-9685

Research interests: Cellular Physiology & Molecular Dynamics   

Schools\degrees:  Ph.D., The Johns Hopkins University 
Training: Postdoctoral Scholar, GI Division, The Johns Hopkins University School of Medicine 

Research summary

Dr. Cha is interested in the mechanisms and molecular dynamics of Sodium Proton Exchanger 3 (NHE3) regulation in the epithelial cells. To study the role of NHE3 dynamics in diarrheal disease, he is using several biophysical techniques such as FRAP (Fluorescence Recovery After Photobleaching), FRET (Fluorescence Resonance Energy Transfer) and Quantum Dot Tracking. 

Journal citations  

  1. Yang J, Singh V, Cha B, Chen TE, Sarker R, Murtazina R, Jin S, Zachos NC, Patterson GH, Tse CM, Kovbasnjuk O, Li X, Donowitz M., (2013) NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells. J Biol Chem. 288(23):16960-74.
  2. Zizak M, Chen T, Bartonicek D, Sarker R, Zachos NC, Cha B, Kovbasnjuk O, Korac J, Mohan S, Cole R, Chen Y, Tse CM, Donowitz M., (2012) Calmodulin kinase II constitutively binds, phosphorylates, and inhibits brush border Na+/H+ exchanger 3 (NHE3) by a NHERF2 protein-dependent process, J Biol Chem. 287(16):13442-56.
  3. *Zhu X, *Cha B, Zachos NC, Sarker R, Chakraborty M, Chen TE, Kovbasnjuk O, Donowitz M, (2011) Elevated calcium acutely regulates dynamic interactions of NHERF2 and NHE3 proteins in opossum kidney (OK) cell microvilli, J. Biol Chem., 286(40): 34486-96. * Equally contributed.
  4. Lin Z, Jin S, Duan X, Wang T, Martini S, Hulamm P, Cha B, Hubbard A, Donowitz M, Guggino SE (2011) Choloride channel (Clc)-5 is necessary for exocytic trafficking of Na+/H+ exchanger 3 (NHE3), J. Biol Chem., 286(26): 22833-45.
  5. Sarker R, Valkhoff VE, Zachos NC, Lin R, Cha B, Chen TE, Guggino S, Zizak M, Dejonge H, Hogema B, Donowitz M (2011) NHERF1 and NHERF2 are Necessary for Multiple but Usually Separate Aspects of Basal and Acute Regulation of NHE3 Activity. Am J Physiol Cell Physiol.  300(4) C771-82
  6. Lin R, Murtazina R, Cha B, Chakraborty M, Sarker R, Chen TE, Lin Z, Hogema BM, de Jonge HR, Seidler U, Turner JR, Li X, Kovbasnjuk O, Donowitz M. (2011) D-Glucose Acts via Sodium/Glucose Cotransporter 1 to increase NHE3 in Mouse Jejunal Brush Border by a Na+/H+ Exchange Regulatory Co-Factor 2-Dependent Process. Gastroenterology, 140(2): 560-71.
  7. Mohan S, Tse CM, Gabelli SB, Sarker R, Cha B, Fahie K, Nadella M, Zachos NC, Tu-Sekine B, Raben D, Amzel LM, Donowitz M. (2010) NHE3 activity is dependent on direct phosphoinositide binding at the N terminus of its intracellular cytosolic region. J. Biol. Chem. 285(45): 34566-78.
  8. Cha B, Zhu XC, Chen W, Jones M, Ryoo S, Zachos NC, Chen TE, Lin R, Sarker R, Kenworthy AK. Tse M, Kovbasnjuk O, Donowitz M. (2010) NHE3 mobility in brush borders increases upon NHERF2-dependent stimulation by lysophosphatidic acid. J Cell Sci. 123(14): 2434-43.
  9. Weinman EJ, Steplock D, Cha B, Kovbasnjuk O, Frost NA, Cunningham R, Shenolikar S, Blanpied TA, Donowitz M., (2009) PTH transiently increases the percent mobile fraction of Npt2a in OK cells as determined by FRAP. Am J Physiol Renal Physiol. F1560-5.
  10. Zachos NC, van Rossum DB, Li X, Caraveo G, Sarker R, Cha B, Mohan S, Desiderio S, Patterson RL, Donowitz M., (2009) Phospholipase C-{gamma} Binds Directly to the Na+/H+ Exchanger 3 and Is Required for Calcium Regulation of Exchange Activity.,  J. Biol. Chem. 284(29):19437-44
  11. Donowitz M, Mohan S, Zhu CX, Chen TE, Lin R, Cha B, Zachos NC, Murtazina R, Sarker R, Li X., (2009) NHE3 regulatory complexes. Exp Biol. 212(11):1638-46.
  12. Zachos, N., Hodson, C., Thelin, W. R., Kovbasnjuk, O., Cha, B., Li, X., Milgram, S., and Donowitz, M., (2008) Elevated Intracellular Calcium Stimulates NHE3 Activity Via an IKEPP (NHERF4) Dependent Mechanism, Cell Physiol Biochem. 22(5-6):693-704
  13. Rafiq Sarker, Mads Grønborg, Boyoung Cha, Yueping Chen, Sachin Mohan, Akhilesh Pandey, David Litchfield, Mark Donowitz, and Xuhang Li. (2008) CK2 binds to the C-terminus of NHE3 and stimulates NHE3 basal activity by phosphorylating a separate site, Mol. Biol. Cell 19(9):3859-70.
  14. Hirsh AJ, Zhang J, Zamurs A, Fleegle J, Thelin WR, Caldwell RA, Sabater JR, Abraham WM, Donowitz M, Cha B, Johnson KB, St George JA, Johnson MR, Boucher RC. (2008) Pharmacological properties of N-(3,5-diamino-6-chloropyrazine-2-carbonyl)-N'-4-[4-(2,3-dihydroxypropoxy)phenyl]butyl-guanidine methanesulfonate (552-02), a novel epithelial sodium channel blocker with potential clinical efficacy for cystic fibrosis lung disease. J Pharmacol Exp Ther. 325(1), 77-88.
  15. Cha, B and Donowitz, M, (2008)  The epithelial BB Na/H exchanger NHE3 is associated with the actin cytoskeleton by binding to ezrin both directly and indirectly via PDZ domain containing NHERF proteins, Clin Exp Pharmacol Physiol. 35(8):863-71.
  16. Cha, B., Tse, M., Yun, C., Kovbasnjuk, O., Mohan, S., Hubbard, A., Arpin, M. and Donowitz, M. (2006) The NHE3 Juxtamembrane C-Terminus Directly Binds Ezrin: Dual Role In NHE3 Trafficking and Movement In the Brush Border, Mol. Biol. Cell 17, 2661-2673.
  17. Donowitz, M., Cha, B., Zachos, N.C., Brett, C.L., Sharma, A., Tse, C.M., and Li, X. (2005). NHERF family and NHE3 regulation. J. Physiol. 567, 3-11
  18. Cha, B., Kim, J.H., Hut, H., Hogema, B.M., Nadarja, J., Zizak, M., Cavet, M., Kwon-Lee-W., Lohmann, S.M., Smolenski, A., Tse, C.M., Yun, C., de Jonge, H.R., and Donowitz, M.  (2005). cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein. J. Biol. Chem. 280, 16642-16650.
  19. Cha, B., Kenworthy, A., Murtazina, R., and Donowitz, M. (2004). The lateral mobility of NHE3 on the apical membrane of renal epithelial OK cells is limited by the PDZ domain proteins NHERF1/2, but is dependent on an intact actin cytoskeleton as determined by FRAP. J. Cell Sci. 117, 3353-3365.
  20. Lee-Kwon. W, Kim, J.H., Choi, J. W., Kawano, K., Cha, B., Dartt, D.A., Zoukhri, D., and Donowitz, M. (2003) Ca2+-dependent inhibition of NHE3 requires PKC  which binds to E3KARP to decrease surface NHE3 containing plasma membrane complexes. Am J Physiol. Cell Physiol 285, C1527-C1536.
  21. Cha, B., Oh, S., Shanmugaratnam, J., Donowitz, M., and Yun, C.C. (2003). Two histidine residues in the juxta-membrane cytoplasmic domain of Na+/H+ exchanger isoform 3 (NHE3) determine the set point. J. Membr. Biol. 191, 49-58.
  22. Lee-Kwon W, Johns D. C., Cha B., Cavet M., Park J., Tsichlis P., Donowitz M. (2001) Constitutively active phosphatidylinositol 3-kinase and AKT are sufficient to stimulate the epithelial Na+/H+ exchanger 3, J. Biol. Chem., 276, 31296 – 304.
  23. Palmer, A., B. Cha and D. Wirtz. (1998) Structure and Dynamics of Actin Filaments Solutions in the Presence of Latrunculin A, J. Polym. Science, 36, 3007 – 3015.
  24. Nahm K, J. S. Kim, K. B. Lee, D. W. Shong, B. Y. Cha and C. K. Kim. (1990) Superconductivity of (Y1-xREx) Ba2Cu3O7-δ system, J. Mat. Science 25, 1762 – 1764
  25. Nahm K., B. Y. Cha and C. K. Kim (1989) Dependence of Tc on Pr concentration in the high Tc superconductor (Y1-xPrx)Ba2Cu3O7-δ, Solid State Commun., 72, 559 – 561.
  26. Jung Soo Kim, Choon Mahn Park, Sung Ho Kim, Kyeong Bock Lee, Bo Young Cha, Chul Koo Kim, Kyun Nahm (1989) Superconducting Properties of High Tc Superconductor (Y1-xREx)Ba2Cu3O7-δ(RE=Er, Dy) J. of Korean Physical Society, 22, 234 – 241.
  27. Kyeong Bock Lee , Jung Soo Kim, Deoug Wha Shong, Bo Young Cha, Kyun Nahm, Chul Koo Kim (1988) The Substitution Effect of Cu with V in the High Tc Superconductor Yba2Cu3O7-δ, Sae Mul Li, 28, 587 – 593.
  28. Moon Kyu Kim, Bo Young Cha, Kyeong Bock Lee, Ho Hyun Kim, Chul Koo Kim, Kyun Nahm (1988) Magnetic Properties of the Superconducting Yba2Cu3Q7-δ With Impurity Phases, J. of Korean Physical Society, 21, 278 – 283.
  29. B. Y. Cha and J. H. Yee (1985) Finite Temperature Effects on the Energy Levels of Hydrogen-Like Atoms. Phys. Rev. D., 32, 1038 – 1039.