Targeted Testing Technical Details
Select each test for methods and additional details.
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- Test Method: DNA extraction (if applicable); amplification of the specified region by polymerase chain reaction (PCR); cycle sequencing of each segment in the forward and reverse directions; analysis of each sequence by capillary electrophoresis; review of sequence data (human reference genome: GRCh37/hg19) by multiple staff members.
- Analytical Sensitivity: > 99% accuracy for nucleotides evaluated; our analysis parameters are not designed to detect mosaicism.
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Test Method: DNA extraction (if applicable); multiplex amplification of fifteen polymorphic markers and one sex determination marker in each sample; capillary electrophoresis of both samples; analysis and review by multiple staff members.
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- Test Method: DNA extraction (if applicable); amplification of the specified region by polymerase chain reaction (PCR); cycle sequencing of each segment in the forward and reverse directions; analysis of each sequence by capillary electrophoresis; review of sequence data (human reference genome: GRCh37/hg19) by multiple staff members. MCC: DNA extraction (if applicable); multiplex amplification of fifteen polymorphic markers and one sex determination marker in each sample; capillary electrophoresis of both samples; analysis and review by multiple staff members.
- Analytical Sensitivity: > 99% accuracy for nucleotides evaluated; our analysis parameters are not designed to detect mosaicism.