Skip Navigation

Masks Strongly Recommended but Not Required in Maryland, Starting Immediately

Due to the downward trend in respiratory viruses in Maryland, masking is no longer required but remains strongly recommended in Johns Hopkins Medicine clinical locations in Maryland. Read more.

Johns Hopkins Medicine

Flow Cytometry Shared Resource Center

Flow Cytometry Shared Resource Center at Johns Hopkins Medicine

Johns Hopkins Medicine Campus

We are located on the 10th floor of the Richard Starr Ross Research Building (Room S1071A). The Core is managed by Division of Hematology, Department of Medicine. The Core offers state-of-the-art flow cytometry cell analysis and high-speed sorting to the research community at Hopkins.

We are currently equipped with BD FACSAria IIu Cell Sorter with three lasers (405-, 488-, and 633 nm) (9 Colors) capable of 4 way sorting. The Core is also equipped with analyzers: Cytek Aurora with four lasers (405-, 488-, 561-, and 640-nm) (48 fluorescence channels and three scatters); BD LSR II with four lasers (355-, 405-, 488-,and 633-nm) (13 colors) and BD FACSCalibur with two lasers (488 nm and 633 nm) (4 colors).

Quick Links

Instruments

All analyzers are accessible for unassisted use 24 hours a day, 7 days a week.

Please acknowledge the use of the JHU Ross Flow Cytometry Core in your publications. If you used Aurora, please cite “Aurora is funded by a NIH Grant (S10OD026859).”

Billing Policies and Fees

Biosafety Policies

The Flow Cytometry Core is a BSL-2 Facility to handle human cells, and subject to the rules, regulations, guidelines that apply to the use of biohazardous materials in all research laboratories. As a multi-use facility, anyone using the facility must have their proposal approved separately by the IRB (human), Animal Review Committee, and Biohazards Review Committee. The RFCC Director, Manager and staff members undergo training sponsored by the Dean’s Office in use of human subject materials, animals and biohazards. Our Core asks all investigators to review and comply with the Biosafety requirements of Johns Hopkins University, and checks that their approvals are current, but is not involved with the applications for the individual users.

The current setup for the core prevents us from running samples that contain radioactivity or potentially infectious agents to humans.

 

Scheduling

Contact Information

Please do not hesitate to contact us if you have any questions about potential uses of flow cytometry for your research projects, how to appropriately design panels for flow cytometry, how to analyze flow data, and prepare for sorting.  We also provide data acquisition and analysis completed by Core staff if you have little flow background and short projects.

Resources

Bayview Flow Cytometry Shared Resource Center (BFCSRC)

The Johns Hopkins Bayview Immunomics Core (BIC) was formed to support the research initiatives of Johns Hopkins Bayview Faculty and staff. We are committed to providing state-of-the art technology and staff support to address current flow cytometry, cell sorting, immunomics applications and new strategies.

About this flow cytometry service
bayview hospital