The Flow Cytometry Shared Resource provides state-of-the-art cell sorting and analysis capability to the members of the SKCCC. The Resource operates two cell sorters, a new three laser BD Aria II, and a first generation FacsAria 6-color sorter with digital acquisition capabilities. These units are maintained and managed by dedicated operators. In addition, the Shared Resource maintains a new 14 color BD LSR II analyzer and three FacsCalibur analysis units; these 4-color machines are user-operated and provide a uniform platform to study cancer cells as well as the immune response to cancer. Capabilities include cell-cycle analysis, intracellular staining for cytokines and kinases, and cell-surface marker analysis. The cell-sorters and analyzers are centrally located on the fourth floor of the Bunting Blaustein Cancer Research Building. This arrangement allows for the Resource manager to maintain qulaity control over the instrumentation as well as ensuring continuous high level of service. Overall, this Core provides essential services for SKCCC investigators from a diverse array of disciplines who seek to more deeply understand the biology and treatment of cancer.
Julie Nauroth, Ph.D., Resource Director
Ada Tam, Resource Manager