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Dr. Jinong Li


Assistant Professor of Pathology

Research Summary

Clinical proteomics aims to scan the realm of expressed proteins to identify biomarkers that can answer specific clinical questions. The most obvious are markers that can be used for diagnosis or prognosis. The Center for Biomarker Discovery established in 2001 is a high-throughput proteomic facility where hundreds of biological samples can be processed and analyzed using the SELDI protein chip/mass spectrometry system (SELDI: surface enhanced laser desorption /ionization). Utilizing bioinformatics tools, complex protein profiles from different diagnostic groups can be compared and biomarkers of interest identified. My current research projects include investigation of serum markers for the early detection of breast cancer, surrogate endpoints in breast cancer prevention studies using the intraductal approach, as well as pharmacoproteomic studies of various clinical trials. 


EM 5/06

Journal Citations

Addlagatta, A., Hu, X., Liu, J. O. & Matthews, B. W. (2005). Structural basis for the functional differences between type I and type II human methionine aminopeptidases. Biochemistry 44, 14741-9.

Addlagatta, A., Quillin, M. L., Omotoso, O., Liu, J. O. & Matthews, B. W. (2005). Identification of an SH3-binding motif in a new class of methionine aminopeptidases from Mycobacterium tuberculosis suggests a mode of interaction with the ribosome. Biochemistry 44, 7166-74.

Allen, M. D., DiPilato, L. M., Rahdar, M., Ren, Y. R., Chong, C., Liu, J. O. & Zhang, J. (2006). Reading dynamic kinase activity in living cells for high-throughput screening. ACS Chem Biol 1, 371-6.

Byrne, S. T., P. Gu, J. Zhou, S. M. Denkin, C. Chong, D. Sullivan, J. O. Liu, and Y. Zhang. 2007. Pyrrolidine dithiocarbamate and diethyldithiocarbamate are active against growing and nongrowing persister Mycobacterium tuberculosis. Antimicrob Agents Chemother 51:4495-7.

Chong, C. R., J. Xu, J. Lu, S. Bhat, D. J. Sullivan, Jr., and J. O. Liu. 2007. Inhibition of angiogenesis by the antifungal drug itraconazole. ACS Chem Biol 2:263-70.

Chong, C. R., Qian, D. Z., Pan, F., Wei, Y., Pili, R., Sullivan, D. J., Jr. & Liu, J. O. (2006). Identification of type 1 inosine monophosphate dehydrogenase as an antiangiogenic drug target. J Med Chem 49, 2677-80.

Dang, Y., Kedersha, N., Low, W. K., Romo, D., Gorospe, M., Kaufman, R., Anderson, P. & Liu, J. O. (2006). Eukaryotic initiation factor 2alpha-independent pathway of stress granule induction by the natural product pateamine A. J Biol Chem 281, 32870-8.

Galban, S., Kuwano, Y., Pullmann, R., Jr., Martindale, J.L., Kim, H.H., Lal, A., Abdelmohsen, K., Yang, X., Dang, Y., Liu, J.O., Lewis, S.M., Holcik, M., and Gorospe, M. (2008). RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alpha. Mol Cell Biol 28, 93-107.

Galban, S., Y. Kuwano, R. Pullmann, Jr., J. L. Martindale, H. H. Kim, A. Lal, K. Abdelmohsen, X. Yang, Y. Dang, J. O. Liu, S. M. Lewis, M. Holcik, and M. Gorospe. 2008. RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alpha. Mol Cell Biol 28:93-107.

Han, A., He, J., Wu, Y., Liu, J. O. & Chen, L. (2005). Mechanism of recruitment of class II histone deacetylases by myocyte enhancer factor-2. J Mol Biol 345, 91-102.

Hoque, M.O., Kim, M.S., Ostrow, K.L., Liu, J., Wisman, G.B., Park, H.L., Poeta, M.L., Jeronimo, C., Henrique, R., Lendvai, A., Schuuring, E., Begum, S., Rosenbaum, E., Ongenaert, M., Yamashita, K., Califano, J., Westra, W., van der Zee, A.G., Van Criekinge, W., and Sidransky, D. (2008). Genome-wide promoter analysis uncovers portions of the cancer methylome. Cancer Res 68, 2661-2670.

Hu, X. V., X. Chen, K. C. Han, A. S. Mildvan, and J. O. Liu. 2007. Kinetic and mutational studies of the number of interacting divalent cations required by bacterial and human methionine aminopeptidases. Biochemistry 46:12833-43.

Hu, X., Addlagatta, A., Lu, J., Matthews, B. W. & Liu, J. O. (2006). Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression. Proc Natl Acad Sci U S A 103, 18148-53.

Hu, X., Addlagatta, A., Matthews, B. W. & Liu, J. O. (2006). Identification of pyridinylpyrimidines as inhibitors of human methionine aminopeptidases. Angew Chem Int Ed Engl 45, 3772-5.

Hu, X., Y. Dang, K. Tenney, P. Crews, C. W. Tsai, K. M. Sixt, P. A. Cole, and J. O. Liu. 2007. Regulation of c-Src nonreceptor tyrosine kinase activity by bengamide A through inhibition of methionine aminopeptidases. Chem Biol 14:764-74.

Kim, H., Kim, B. Y., Soh, J. W., Cho, E. J., Liu, J. O. & Youn, H. D. (2006). A novel function of Nur77: physical and functional association with protein kinase C. Biochem Biophys Res Commun 348, 950-6.

Liu, J. O. (2005). The yins of T cell activation. Sci STKE 2005, re1.

Liu, J., H. J. Martin, G. Liao, and S. D. Hayward. 2007. The Kaposi's sarcoma-associated herpesvirus LANA protein stabilizes and activates c-Myc. J Virol 81:10451-9.

Liu, J., H. Martin, M. Shamay, C. Woodard, Q. Q. Tang, and S. D. Hayward. 2007. Kaposi's sarcoma-associated herpesvirus LANA protein downregulates nuclear glycogen synthase kinase 3 activity and consequently blocks differentiation. J Virol 81:4722-31.

Low, W. K., Dang, Y., Schneider-Poetsch, T., Shi, Z., Choi, N. S., Merrick, W. C., Romo, D. & Liu, J. O. (2005). Inhibition of eukaryotic translation initiation by the marine natural product pateamine A. Mol Cell 20, 709-22.

Low, W. K., Y. Dang, S. Bhat, D. Romo, and J. O. Liu. 2007. Substrate-dependent targeting of eukaryotic translation initiation factor 4A by pateamine A: negation of domain-linker regulation of activity. Chem Biol 14:715-27.

Low, W. K., Y. Dang, T. Schneider-Poetsch, Z. Shi, N. S. Choi, R. M. Rzasa, H. A. Shea, S. Li, K. Park, G. Ma, D. Romo, and J. O. Liu. 2007. Isolation and identification of eukaryotic initiation factor 4A as a molecular target for the marine natural product Pateamine A. Methods Enzymol 431:303-24.

Lu, J., Chong, C. R., Hu, X. & Liu, J. O. (2006). Fumarranol, a rearranged fumagillin analogue that inhibits angiogenesis in vivo. J Med Chem 49, 5645-8.

Ma, D., Zou, B., Cai, G., Hu, X. & Liu, J. O. (2006). Total synthesis of the cyclodepsipeptide apratoxin A and its analogues and assessment of their biological activities. Chemistry 12, 7615-26.

McMahon, M. A., B. L. Jilek, T. P. Brennan, L. Shen, Y. Zhou, M. Wind-Rotolo, S. Xing, S. Bhat, B. Hale, R. Hegarty, C. R. Chong, J. O. Liu, R. F. Siliciano, and C. L. Thio. 2007. The HBV drug entecavir - effects on HIV-1 replication and resistance. N Engl J Med 356:2614-21.

McMahon, M.A., Siliciano, J.D., Lai, J., Liu, J.O., Stivers, J.T., Siliciano, R.F., and Kohli, R.M. (2008). The antiherpetic drug acyclovir inhibits HIV replication and selects the V75I reverse transcriptase multidrug resistance mutation. J Biol Chem 283, 31289-31293.

Pan, F., L. Sun, D. B. Kardian, K. A. Whartenby, D. M. Pardoll, and J. O. Liu. 2007. Feedback inhibition of calcineurin and Ras by a dual inhibitory protein Carabin. Nature 445:433-6.

Pan, F., Means, A. R. & Liu, J. O. (2005). Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation. Embo J 24, 2104-13.

Park, H.L., Kim, M.S., Yamashita, K., Westra, W., Carvalho, A.L., Lee, J., Jiang, W.W., Baek, J.H., Liu, J., Osada, M., Moon, C.S., Califano, J.A., Mori, M., and Sidransky, D. (2008). DCC promoter hypermethylation in esophageal squamous cell carcinoma. Int J Cancer 122, 2498-2502.

Peddibhotla, S., Y. Dang, J. O. Liu, and D. Romo. 2007. Simultaneous arming and structure/activity studies of natural products employing O-H insertions: an expedient and versatile strategy for natural products-based chemical genetics. J Am Chem Soc 129:12222-31

Pei, Y., Zhu, P., Dang, Y., Wu, J., Yang, X., Wan, B., Liu, J.O., Yi, Q., and Yu, L. (2008). Nuclear export of NF90 to stabilize IL-2 mRNA is mediated by AKT-dependent phosphorylation at Ser647 in response to CD28 costimulation. J Immunol 180, 222-229.

Ren, Y.R., Pan, F., Parvez, S., Fleig, A., Chong, C.R., Xu, J., Dang, Y., Zhang, J., Jiang, H., Penner, R., and Liu, J.O. (2008). Clofazimine inhibits human Kv1.3 potassium channel by perturbing calcium oscillation in T lymphocytes. PLoS ONE 3, e4009.

Tang, P., F. Mamdani, X. Hu, J. O. Liu, and B. Yu. 2007. Synthesis of OSW saponin analogs with modified sugar residues and their antiproliferative activities. Bioorg Med Chem Lett 17:1003-7.

Tang, P., Mamdani, F., Hu, X., Liu, J. O. & Yu, B. (2006). Synthesis of OSW saponin analogs with modified sugar residues and their antiproliferative activities. Bioorg Med Chem Lett.

Wang, Y., Zhang, F., Zhang, Y., Liu, J.O., and Ma, D. (2008). Synthesis and antitumor activity of cyclodepsipeptide zygosporamide and its analogues. Bioorg Med Chem Lett 18, 4385-4387.

Wu, G., Xing, M., Mambo, E., Huang, X., Liu, J., Guo, Z., Chatterjee, A., Goldenberg, D., Gollin, S. M., Sukumar, S., Trink, B. & Sidransky, D. (2005). Somatic mutation and gain of copy number of PIK3CA in human breast cancer. Breast Cancer Res 7, R609-16.

Zhang, H., Qian, D.Z., Tan, Y.S., Lee, K., Gao, P., Ren, Y.R., Rey, S., Hammers, H., Chang, D., Pili, R., Dang, C.V., Liu, J.O., and Semenza, G.L. (2008). Digoxin and other cardiac glycosides inhibit HIF-1alpha synthesis and block tumor growth. Proc Natl Acad Sci U S A 105, 19579-19586.

Kuhajda, F. P., Pizer, E. S., Li, J. N., Mani, N. S., Frehywot, G. L., & Townsend, C. A. 2000. Synthesis and anti-tumor activity of an inhibitor of fatty acid synthase. P. Natl. Acad. Sci. USA. 97:3450-3454.

Li, J. N., Gorospe, M., Chrest, F. J., Kumaravel, T. S., Evans, M. K., Han, W. F., et al. 2001. Pharmacological inhibition of fatty acid synthase activity produces both cytostatic and cytotoxic effects modulated by p53. Cancer Res. 61:1493-1499.

Li, J. N., Mahmoud, M. A., Han, W. F., Ripple, M., & Pizer, E. S. 2000. Sterol regulatory element-binding protein-1 participates in the regulation of fatty acid synthase expression in colorectal neoplasia. Exp. Cell Res. 261:159-165.

Li, J. N., Orlandi, R., White, C. N., Rosenzweig, J., Zhao, J., Seregni, E., et al. 2005. Independent validation of candidate breast cancer serum biomarkers identified by mass spectrometry. Clin. Chem. 51:2229-2235.

Li, J. N., White, N., Zhang, Z., Rosenzweig, J., Mangold, L. A., Partin, A. W., et al. 2004. Detection of prostate cancer using serum proteomics pattern in a histologically-confirmed population. J. Urol. 171:1782-1787.

Li, J. N., Zhang, Z., Rosenzweig, J., Wang, Y. Y., & Chan, D. W. 2002. Proteomics and bioinformatics approaches for identification of serum biomarkers to detect breast cancer. Clin. Chem. 48:1296-1304.

Li, J. N., Zhao, J., Yu, X., Lange, J., Kuerer, H., Krishnamurthy, S., et al. 2005. Identification of biomarkers for breast cancer in nipple aspiration and ductal lavage fluid. Clin. Cancer Res. 11:8312-8320.

Zhang, Z., Bast, R., Yu, Y., Li, J. N., Sokoll, L., Rai, A. J., et al. 2004. Three biomarkers discovered from serum proteomic analysis for the detection of early stage ovarian cancer. Cancer Res. 64:5882-5890.


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