Skip Navigation
 
 
 
 
 
Print This Page
Share this page: More
 

Philip A. Cole

Cole

Department Affiliation: Pharmacology
Degree: M.D., Ph.D., Johns Hopkins University
Rank: Director and E.K. Marshall and Thomas H. Maren Professor
Telephone Number: 410-614-8849
Fax Number: 410-955-3023
E-mail address: pcole@jhmi.edu
School of Medicine Address: 316 Hunterian, Johns Hopkins School of Medicine, 725 N. Wolfe St., Baltimore, MD, 21205

Click here for a list of staff in the Cole Lab.

Chemical and biochemical approaches in the study of signal transduction, gene regulation, and metabolism.

Our lab studies the chemical biology of protein post-translational modification and regulation.  We study the structure, function, and regulation of protein kinases and protein phosphatases by phosphorylation, acetylation, and glycosylation.  In this regard, we have developed a series of technologies including expressed protein ligation, chemical rescue of mutant enzymes, and bisubstrate analog inhibitors that we apply to the analysis of signaling proteins that help clarify substrate selectivity, activation state, and function.  Our lab also studies histone acetyltransferases and demethylases and how they contribute to the regulation of gene expression and signaling.  We have designed a series of selective inhibitors of these enzymes as tools for pharmacologic research.  We employ and develop novel protein chemistry and proteomics methods including mass spectrometry to analyze the function of signaling enzymes on a broad scale.  In addition, we are studying the mechanism and function of acyltransferases involved in circadian rhythm and metabolism using chemical biology strategies.

Representative Publications:

  • DeAngelis, J., Gastel, J., Klein, D.C., Cole, P.A.  Kinetic analysis of the catalytic mechanism of serotonin N-acetyltransferase.  J. Biol. Chem. 273:3045-3050, 1998. Pub Med Reference
  •  
  • Muir, T.W., Sondhi, D., Cole, P.A.  Expressed protein ligation: A general method for protein engineering.  Proc. Natl. Acad. Sci. USA 95:6705-6710, 1998. [Commentary: Science (1998) 281, 367-368]  Pub Med Reference
  •  
  • Khalil, E. and Cole, P.A.  A potent inhibitor of the melatonin rhythm enzyme.  J. Am. Chem. Soc. 120:6195-6196, 1998. [Commentary: Science News (1998) 154, 6-7]  Pub Med Abstract not available.
  •  
  • Kim, K. and Cole, P.A.  Kinetic analysis of a protein tyrosine kinase transition state in the forward and reverse directions.  J. Am. Chem. Soc. 120:6851-6858, 1998.  Pub Med Abstract not available.
  •  
  • Khalil, E., DeAngelis, J., Cole, P.A.  Indole-amine analogs as probes of the substrate selectivity and catalytic mechanism of serotonin N-acetyltransferase.  J. Biol. Chem. 273:30321-30327, 1998.  Pub Med Reference
  •  
  • Cole, P.A., Sondhi, D., Kim, K.  Chemical approaches to the study of protein kinases and their implications for mechanism and inhibitor design.  Pharmacology and Therapeutics 82:219-229, 1999.  Pub Med Reference
  •  
  • Kim, K. and Cole, P.A.  Synthesis of (2S, 3R)-beta-methyltyrosine catalyzed by tyrosine phenol-lyase bioorg.  Med. Chem. Lett. 9:1205-1208, 1999.  Pub Med Reference
  •  
  • Sondhi, D. and Cole, P.A.  Domain interactions in protein tyrosine kinase Csk.  Biochemistry 38:11147-11155, 1999.  Pub Med Reference
  •  
  • Khalil, E.M., De Angelis, J., Ishii, M., Cole, P.A.  Mechanism-based inhibition of the melatonin rhythm enzyme: Pharmacologic exploitation of active site functional plasticity.  Proc. Nat. Acad. Sci. USA 96:12418-12423, 1999.  Pub Med Reference
  •  
  • Lau, O.D., Kundu, T.K., Soccio, R.E., Ait-Si-Ali, S., Khalil, E., Vassilev, A., Wolffe, A.P., Nakatani, Y., Roeder, R.G., Cole, P.A.  HATs off: Selective synthetic inhibitors of the histone acetyltransferases p300 and PCAF.  Mol. Cell 5:589-595, 2000.  Pub Med Reference
  •  
  • Blaustein, R.O., Cole, P.A., Williams, C., Miller, C.  Mapping K+ channels with molecular tape-measures.  Nat. Struct. Biol. 7:309-311, 2000.  Pub Med Reference
  •  
  • Kim, K., Parang, K., Lau, O.D., Cole, P.A.  Tyrosine analogs as alternative substrates for protein tyrosine kinase Csk: Insights into substrate selectivity and catalytic mechanism.  Bioorg. Med. Chem. 8:1263-1268, 2000.  Pub Med Reference
  •  
  • Lau, O.D., Courtney, A.D., Vassilev, A., Marzilli, L.A., Cotter, R.J., Nakatani, Y., Cole, P.A.  PCAF histone acetyltransferase processing of a peptide substrate: Kinetic analysis of the catalytic mechanism.  J. Biol. Chem. 275:21953-21959, 2000.  Pub Med Reference
  •  
  • Kundu, T.K., Palhan, V.B., Wang, Z.Y., An, W., Cole, P.A., Roeder, R.G.  Activator-dependent transcription from chromatin in vitro involving targeted histone acetylation by p300.  Mol. Cell 6(3):551-561, 2000.  Pub Med Reference
  •  
  • Ablooglu, A.J., Till, J.H., Kim, K., Parang, K., Cole, P.A., Hubbard, S.R., and Kohanski, R.A.  Probing the catalytic mechanism of the insulin receptor kinase with a tetrafluorotyrosine-containing peptide substrate.  J. Biol. Chem. 275:30394-30398, 2000.  Pub Med Reference
  •  
  • Williams D.M., Wang, D., Cole, P.A.  Chemical rescue of a mutant protein tyrosine kinase.  J. Biol. Chem. 275:38127-38130, 2000.  Pub Med Reference
  •  
  • Parang, K., Till, J.H., Ablooglu, A.J., Kohanski, R.A., Hubbard, S.R., Cole, P.A.  Mechanism-based design of a protein kinase inhibitor.  Nat. Struct. Biol. 8:37-41, 2001.  [Commentary: Double Trouble, Nat. Struct. Biol. 8:16-18 (2001)]  Pub Med Reference
  •  
  • Wang, D., Huang, X.-Y., Cole, P.A.  Molecular determinants for Csk-catalyzed tyrosine phosphorylation of the Src tail.  Biochemistry 40:2004-2010, 2001.  Pub Med Reference
  •  
  • Zheng, W., Scheibner, K.A., Ho, A.K., Cole, P.A.  Mechanistic studies on the alkyltransferase activity of serotonin N-acetyltransferase.  Chem. Biol. 8(4):379-389, 2001.  Pub Med Reference
  •  
  • Williams, D.M. and Cole, P.A.  Kinase chips hit the proteomics era.  Trends Biochem. Soc. 26(5):271-273, 2001.  Pub Med Reference
  •  
  • Thompson, P.R. and Cole, P.A.  Probing the mechanism of enzymatic phosphoryl transfer with a chemical trick.  Proc. Natl. Acad. Sci. USA 98:8170-8171, 2001.  Pub Med Reference
  •  
  • Kim, C.M. and Cole, P.A.  Bisubstrate ketone analogs as serotonin N-acetyltransferase inhibitors.  J. Med. Chem. 44:2479-2485, 2001.  Pub Med Reference
  •  
  • Thompson, P.R., Kurooka, H., Nakatani, Y., Cole, P.A.  Transcriptional coactivator protein p300: Kinetic characterization of its histone acetyltransferase activity.  J. Biol. Chem. 276:33721-33729, 2001.  Pub Med Reference
  •  
  • Wang, D. and Cole, P.A.  Protein tyrosine kinase Csk-catalyzed phosphorylation of Src containing unnatural tyrosine analogs.  J. Am. Chem. Soc. 123:8883-8886, 2001.  Pub Med Reference    
  •  
  • Lu, W., Gong, D., Bar-Sagi, D., Cole, P.A.  Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling.  Mol. Cell 8:759-769, 2001.  Pub Med Reference
  •  
  • Asahara, H., Santoso, B., Du, K., Cole, P.A., Davidson, I., Montminy, M.  Chromatin dependent cooperativity between constitutive and inducible activation domains in CREB.  Mol. Cell. Biol. 21:7892-7900, 2001.  Pub Med Reference
  •  
  • Shekhtman, A., Ghose, R., Wang, D., Cole, P.A., Cowburn, D.  Novel mechanism of the non-receptor protein tyrosine kinase Csk: Insights from NMR mapping studies and site-directed mutagenesis.  J. Mol. Biol. 314:129-138, 2001.  Pub Med Reference
  •  
  • Polesskaya, A., Naguibneva, I., Duquet, A., Ait-Si-Ali, S., Robin, P., Vervish, A., Cole, P.A., Harel-Bellan, A.  CBP/p300 and muscle differentiation: no HAT, no muscle.  EMBO J. 20:6816-6825, 2001.  Pub Med Reference
  •  
  • Costanzo, A., Merlo, P., Pediconi, N., Fulco, M., Sartorelli, V., Cole, P.A., Fontemaggi, G., Fanciulli, M., Schiltz, L., Blandino, G., Balsano, C., Levrero, M.  DNA damage-dependent acetylation of p73 dictates the selective activation of apoptotic target genes.  Mol. Cell 9:175-186, 2002.  Pub Med Reference
  •  
  • Wolf, E., De Angelis, J., Khalil, E.M., Cole, P.A., and Burley, S.K.  X-ray crystallographic studies of serotonin N-acetyltransferase catalysis and inhibition.  J. Mol. Biol. 317:215-224, 2002.  Pub Med Reference
  •  
  • Zheng, W. and Cole, P.A.  Serotonin N-acetyltransferase: Mechanism and inhibition.  Curr. Med. Chem. 9:1187-1199, 2002.  Pub Med Reference
  •  
  • Parang, K. and Cole P.A.  Design of bisubstrate analog inhibitors for protein kinases.  Pharmacol. Ther. 93:145-157, 2002.  Pub Med Reference
  •  
  • Scheibner, K.A., DeAngelis, J., Burley, S.K., Cole, P.A.  Investigation of the roles of catalytic residues in serotonin N-acetyltransferase.  J. Biol. Chem. 277:18118-18126, 2002.  Pub Med Reference
  •  
  • Subbaramaiah, K., Cole, P.A., Dannenberg, A.J.  Retinoids and carnosol suppress cyclooxygenase-2 transcription by CBP/p300-dependent and independent mechanisms.  Cancer Research 62:2522-2530, 2002.  Pub Med Reference
  •  
  • Lowry, W.E., Huang, J.J., Ma, Y.-C., Ali, S., Wang, D., Williams, D.M., Okada, M., Cole, P.A., Huang, X.Y.  Csk, a critical link of G protein signals to actin cytoskeletal reorganization.  Dev. Cell 2(6):733-734, 2002.  Pub Med Reference
  •  
  • Lu, H., Pise-Masison, C.A., Fletcher, T.M., Schiltz, R.L., Nagaich, A., Radonovich, M., Hager, G., Cole, P.A., Brady, J.N.  The HAT activity of p300 is required for tax-mediated HTLV-I LTR transcription from a chromatin template.  Mol. Cell. Biol. 22:4450-4462, 2002.  Pub Med Reference
  •  
  • Williams, D.M. and Cole, P.A.  Proton demand inversion in a mutant protein kinase reaction.  J. Am. Chem. Soc. 124:5956-5957, 2002.  Pub Med Reference
  •  
  • Parang, K., Kohn, J., Saldanha, A., Cole, P.A.  Development of photo-cross-linking reagents for protein kinase-substrate interactions.  FEBS Lett. 520:156-160, 2002.  Pub Med Reference
  •  
  • Wang, D., Esselman, W.J., Cole, P.A.  Substrate conformational restriction and CD45-catalyzed dephosphorylation of tail tyrosine-phosphorylated Src protein.  J. Biol. Chem. 277:40428-40433, 2002.  Pub Med Reference
  •  
  • Bandyopadhyay, D., Okan, N.A., Bales, E., Nascimento, L., Cole, P.A., Medrano, E.E.  Down-regulation of p300/CBP HAT activates a senescence checkpoint in human melanocytes.  Cancer Research 62:6231-6239, 2002.  Pub Med Reference
  •  
  • Poux, A.N., Cebrat, M., Kim, C.M., Cole, P.A., Marmorstein, R.  Structure of the GCN5 histone acetyltransferase bound to a bisubstrate inhibitor.  Proc. Natl Acad. Sci. USA 99:14065-14070, 2002.  Pub Med Reference
  •  
  • Zhang, Z., Shen, K., Lu, W., Cole, P.A.  The role of c-terminal tyrosine phosphorylation in the regulation of SHP-1 explored via expressed protein ligation.  J. Biol. Chem. 278:4668-4674, 2003.  Pub Med Reference
  •  
  • Li, J., Lee, K.C., Cole, P.A., Wong, J., Kraus, W.L.  Transcriptional activation by thyroid hormone receptor beta involves chromatin remodeling, histone acetylation, and synergistic stimulation by p300 and SRC coactivators.  Mol. Endocrinol. 17(5):908-922, 2003.  Pub Med Reference
  •  
  • Scheibner, K.A., Zhang, Z., Cole, P.A.  Merging FRET and expressed protein ligation to analyze protein-protein interactions.  Anal. Biochem. 317:226-232, 2003.  Pub Med Reference
  •  
  • Cole, P.A., Courtney, A.D., Zhang, Z., Qiao, Y., Lu, W., Williams, D.M.  Chemical approaches to reversible protein phosphorylation.  Acc. Chem. Res. 36(6):444-452, 2003.  Pub Med Reference
  •  
  • Georges, S.A., Giebler, H.A., Cole, P.A., Luger, K., Laybourn, P.J., Nyborg, J.K.  Tax recruitment of CBP/p300, via the KIX domain, reveals a potent requirement for acetyltransferase activity that is chromatin-dependent and histone tail-independent.  Mol. Cell. Biol. 23:3392-3404, 2003.  Pub Med Reference
  •  
  • Huang, Z.-Q., Li, J., Sachs, L.M., Cole, P.A., Wong, J.  A role for cofactor-cofactor and -histone interactions in targeting p300, SWI/SNF and mediator for transcription.  EMBO J. 22:2146-2155, 2003.  Pub Med Reference
  •  
  • Lu, W., Shen, K. and Cole, P.A.  Chemical dissection of the effects of tyrosine phosphorylation of SHP-2.  Biochemistry 42:5461-5468, 2003.  Pub Med Reference
  •  
  • Cebrat, M., Kim, C.M., Thompson, P.R., Daugherty, M., Cole, P.A.  Synthesis and analysis of potential prodrugs of coenzyme A analogs for the inhibition of histone acetyltransferase p300.  Bioorg. Med. Chem. 11:3307-13, 2003.  Pub Med Reference
  •  
  • Kaehlcke, K., Dorr, A., Hetzer-Egger, C., Kiermer, V., Henklein, P., Schnoelzer, M., Loret, E., Cole, P.A., Verdin, E., Ott, M.  Acetylation of Tat defines a cyclin T1-independent step in HIV transactivation.  Mol. Cell 12:167-76, 2003.  Pub Med Reference
  •  
  • Zheng, W. and Cole, P.A.  Novel bisubstrate analog inhibitors of serotonin N-acetyltransferase: The importance of being neutral.  Bioorg. Chem. 31:398-411, 2003.  Pub Med Reference
  •  
  • Cole, P.A., Shen, K., Qiao, Y., Wang, D.  Protein tyrosine kinases Src and Csk: A tail's tale.  Curr. Opin. Chem. Biol. 7:580-585, 2003.  Pub Med Reference
  •  
  • Zheng, W., Zhang, Z., Ganguly, S., Weller, J.L., Klein, D., Cole, P.A.  Cellular stabilization of the melatonin rhythm enzyme induced by non-hydrolyzable phosphonate incorporation.  Nat. Struct. Biol. 10:1054-1057, 2003. �[Commentary: Baltimore Sun October, 27, 2003, p. 3.  Pub Med Reference
  •  
  • Shen, K. and Cole, P.A.  Conversion of a tyrosine kinase protein substrate to a high affinity ligand by ATP linkage.  J. Am. Chem. Soc. 125:16172-16173, 2003.  Pub Med Reference
  •  
  • Zheng, Y., Thompson, P.R., Cebrat, M., Wang, L., Devlin, M.K., Alani, R.M., Cole, P.A.  Selective HAT inhibitors as mechanistic tools for protein acetylation.  Meth. Enz. 376:188-199, 2004.  Pub Med Reference
  •  
  • Thompson, P.R., Wang, D., Wang, L., Fulco, M., Pediconi, N., Zhang, D., An, W., Ge, Q., Roeder, R.G., Wong, J., Levrero, M., Sartorelli, V., Cotter, R.J., Cole, P.A.  Regulation of p300 HAT domain via a novel activation loop.  Nat. Struct. Mol. Biol. 11:308-315, 2004.  [Commentary: Nature Struct. Mol. Biol. 10, 298-300].  Pub Med Reference
  •  
  • Farrell, C.J., Lee, J.M., Shin, E.-C., Cebrat, M., Cole, P.A., Hayward, S.D.  Inhibition of Epstein-Barr Virus induced growth proliferation by an EBNA2-TAT peptide.  Proc. Natl. Acad. Sci. USA 101:4625-4630, 2004.  Pub Med Reference.
  •  
  • Sagar, V., Zheng, W., Thompson, P.R., Cole, P.A.  Bisubstrate analog structure-activity-relationships for p300 histone acetyltransferase inhibitors.  Bioorg. Med. Chem. 12:3383-3390, 2004.  Pub Med Reference
  •  
  • Hines, A.C. and Cole, P.A.  Design, synthesis and characterization of an ATP-peptide conjugate inhibitor of protein kinase A.  Bioorg. Med. Chem. Lett. 14:2951-2954, 2004.  Pub Med Reference
  •  
  • Hwang, Y. and Cole, P.A.  Efficient synthesis of phosphorylated prodrugs with bis(POM)-phosphoryl chloride.  Org. Letters 6:1555-1556, 2004.  Pub Med Reference
     
  • Shi, Y., Lan, F., Matson, C., Mulligan, P., Whetstine, J.R., Cole, P. A., Casero, R.A., Shi, Y.  Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.  Cell 119:941-953, 2004.  Pub Med Reference
  •  
  • Yoon, H.G., Choi, Y., Cole, P.A., Wong, J.  Reading and function of a histone code involved in targeting corepressor complexes for repression.  Mol. Cell. Biol. 25:324-335, 2005.  Pub Med Reference
  •  
  • Cheung, E., Acevedo, M.L., Cole, P.A., Kraus, W.L.  Altered pharmacology and distinct coactivator usage for estrogen receptor-dependent transcription through activating protein-1.  Proc. Natl. Acad. Sci. USA 102:559-564, 2005.  Pub Med Reference
  •  
  • Wang, D., Thompson, P.R., Cole, P.A., Cotter, R.J.  Post-source decay analysis of a highly acetylated protein using a curved-field reflectron mass spectrometer.  Proteomics 5:2288-2295, 2005.  Pub Med Reference
  •  
  • Zheng, W., Schwarzer, D., LeBeau, A., Weller, J.L., Klein, D.C., Cole, P.A.  Cellular stability of serotonin N-acetyltransferase conferred by Phosphono-difluoromethylene Alanine (Pfa) substitution for Ser205.  J. Biol. Chem. 280:10462-10467, 2005.  Pub Med Reference
  •  
  • Hines, A.C., Parang, K., Kohanski, R.A., Hubbard, S.R., Cole, P.A.  Bisubstrate analog probes for the insulin receptor protein tyrosine kinase: molecular yardsticks for analyzing catalytic mechanism and inhibitor design.  Bioorg. Chem. 33:285-297, 2005.  Pub Med Reference
  • Zheng, Y., Mamdani, F., Toptygin, D., Brand, L. Stivers, J.T., Cole, P.A.  Fluorescence analysis of a dynamic loop in the PCAF/GCN5 histone acetyltransferase.  Biochemistry 44:10501-10509, 2005.  Pub Med Reference

  • Schwarzer, D. and Cole, P.A.  Protein semisynthesis and expressed protein ligation: Chasing a protein’s tail.  Curr. Opin. Chem. Biol. 9:561-569, 2005.  Pub Med Reference

  • Guidez, F., Howell, L., Islam, M., Cebrat, M., Alani, R.M., Ivins, S., Hormaeche, I., McConnell, M. J., Pierce, S., Cole, P.A., Licht, J., Zelent, A.  Histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic zinc finger protein.  Mol. Cell. Biol. 25:5552-5566, 2005.  Pub Med Reference

  • Cleary, J., Sitwala, K.V., Khodadoust, M.S., Kwok, R.P.S., Mor-Vaknin, N., Cebrat, M., Cole, P.A., Markovitz, D.M.  Acetylation by P/CAF drives DEK into interchromatin granule clusters.  J. Biol. Chem. 280:31760-31767, 2005.  Pub Med Reference

  • Shen, K., Hines, A.C., Schwarzer, D., Paumi, K., Cole, P.A.  Protein kinase structure and function analysis using chemical tools.  Biochim. Biophys. Acta 1754:65-78, 2005.  Pub Med Reference

  • Lewczuk, B., Zheng, W., Prusik, M., Cole, P.A., Przybylska-Gornowicz, B.  N-bromoacetyltryptamine strongly and reversibly inhibits melatonin secretion from mammalian pinealocytes.  Neuro. Endocrinol. Lett. 26(5):581-592, 2005.  Pub Med Reference

  • Zheng, Y., Karanam, B., Cebrat, M. Buck, D., Guidez, F., Zelent, A., Alani, R.M., Cole, P.A.  Synthesis and evaluation of a potent and selective cell-permeable p300 histone acetyltransferase inhibitor.  J. Am. Chem. Soc. 127:17182-17183, 2005.  Pub Med Reference

  • Qiao, Y., Molina, H., Pandey, A., Zhang, J., Cole, P.A.  Chemical rescue of a mutant enzyme in living cells.  Science 311:1293-1297, 2006.  Pub Med Reference

  • Levinson, N.M., Kuchmnet, O., Shen, K., Young, M.A., Koldobskiy, M., Karplus, M., Cole, P.A., Kuriyan, J.  A Src-like inactive conformation in the abl tyrosine kinase domain.  PloSBiology 4(5):e144, 2006.  Pub Med Reference

  • Culhane, J.C., Szewczuk, L.M., Liu, X., Da, G., Marmorstein, R., Cole, P.A.  A mechanism-based inactivator for histone demethylase LSD1.  J. Am. Chem. Soc. 128:4356-4357, 2006.  Pub Med Reference   

  • Schwarzer, D., Zhang, Z., Zheng, W., Cole, P.A.  Negative regulation of a tyrosine phosphatase by tyrosine phosphorylation.  J. Am. Chem. Soc. 128:4192-4193, 2006.  Pub Med Reference

  • Bose, R., Molina, H., Patterson, A.S., Bitok, J.K., Periaswamy, B., Bader, J.S., Pandey, A., Cole, P.A.  Phosphoproteomic analysis of Her2/neu signaling and inhibition.  Proc. Natl Acad Sci. USA 103:9773-9778, 2006.  Pub Med Reference   

  • Zhang, X., Gureasko, J., Shen, K., Cole, P.A., Kuriyan, J.  An allosteric mechanism for the activation of the kinase domain of the epidermal growth factor receptor.  Cell 125:1137-1149, 2006. Pub Med Reference  

  • Cheng, K.Y., Noble, M.E.M., Skamnaki, V., Brown, N.R., Lowe, E.D., Kontogiannis, L., Shen, K., Cole, P.A., Siligardi, G., Johnson, L.N.  The role of the phospho-CDK2/cyclin A recruitment site in substrate recognition.  J. Biol. Chem. 281(32):23167-23179, 2006.  Pub Med Reference

  • Bose, R., Holbert, M.A., Pickin, K.A., Cole, P.A.  Protein tyrosine kinase-substrate interactions.  Curr. Op. Struct. Biol. 16:668-675, 2006.  Pub Med Reference

  • Cole, P. A. (2007) 'Chemical Biology and Enzymology, Protein Phosphorylation as a Case Study' in Chemical Biology, eds. Schreiber, Kapoor, Wess; Wiley-VCH, Weinheim.
     
  • Szewczuk, L.M., Tarrant, M.K., Cole, P.A.  Protein phosphorylation by semisynthesis: from paper to practice.  Meth. Enz. 462:1-24, 2009.  Pub Med Reference
     
  • Hwang, Y., Ganguly, S., Ho, A. K., Klein, D.C., Cole, P.A.  Enzymatic and cellular study of a serotonin N-acetyltransferase phosphopantetheine-based prodrug.  Bioorg. Med. Chem. 15:2147-2155, 2007.  Pub Med Reference
     
  • Kenneth, N.S., Ramsbottom, B.A., Gomez-Roman, N., Marshall, L., Zhang, J., Cole, P.A., White, R.J.  Activation of Pol III transcription by c-Myc involves selective acetylation of histone H3 and recruitment of TRAPP, GCN5, and TFIIIB.  Proc. Natl Acad. Sci. USA 104:14917-14922, 2007.  Pub Med Reference
     
  • Karanam, B., Wang, L., Wang, D., Liu, X., Marmorstein, R., Cotter, R., Cole, P.A.  Multiple roles for acetylation in the interaction of p300 HAT with ATF-2.  Biochemistry 46:8207-8216, 2007.  Pub Med Reference
     
  • Hu, X., Dang, Y., Tenney, K., Crews, P., Tsai, C.W., Sixt, K.M., Cole, P.A., Liu, J.O.  Regulation of c-Src non-receptor tyrosine kinase activity by bengamide A through inhibition of methionine aminopeptidases.  Chem. Biol. 14:764-774, 2007.  Pub Med Reference
     
  • Szewczuk, L.M., Culhane, J.C., Yang, M., Majumdar, A., Yu, H., Cole, P.A.  Mechanistic analysis of a suicide inactivator of histone demethylase LSD1.  Biochemistry 46:6892-6902, 2007.  Pub Med Reference
     
  • Yang, M., Culhane, J.C., Szewczuk, L.M., Gocke, C.B., Brautigam, C.A., Tomchick, D.R., Machius, M., Cole, P. A.*, Yu, H.*  Structural basis of histone demethylation by LSD1 revealed by suicide inactivation.  Nat. Struct. Mol. Biol. 14:535-539, 2007. (*co-corresponding author)  Pub Med Reference
     
  • Yang, M., Culhane, J.C., Szewczuk, L.M., Jalli, P., Ball, H.L., Machius, M., Cole, P.A., Yu, H.  Structural basis for inhibition of the LSD1 histone demethylase by the antidepressant trans-2-phenylcyclopropylamine.  Biochemistry 46:8058-8065, 2007.  Pub Med Reference
     
  • Muratore, K. and Cole, P.A.  A lock on phosphotyrosine signaling.  ACS Chemical Biology 2:454-456, 2007.  Pub Med Reference
     
  • Zhang, X., Pickin, K.A., Bose, R., Jura, N., Cole, P.A., Kuriyan, J.  Inhibition of the EGF receptor by blockage of an activating kinase domain interface.  Nature 450:741-744, 2007.  Pub Med Reference
     
  • Hwang, Y., Thompson, P.R., Wang, L., Jiang, L., Kelleher, N.L., Cole, P.A.  A selective chemical probe for coenzyme-A requiring enzymes.  Angewandte Chemie Int. Ed. 46:7621-7624, 2007.  Pub Med Reference
     
  • Culhane, J.C. and Cole, P.A.  LSD1 and the chemistry of histone demethylation.  Curr. Op. Chem. Biol. 11:561-568, 2007.  Pub Med Reference
     
  • Szewczuk, L.M., Saldanha, S.A., Ganguly, S., Bowers, E.M., Javoroncov, M., Karanam, B., Culhane, J.C., Holbert, M.A., Klein, D.C., Abagyan, R., Cole, P.A.  De novo discovery of serotonin N-acetyltransferase inhibitors.  J. Med. Chem. 50:5330-5338, 2007.  Pub Med Reference
     
  • Liu, X., Wang, L., Zhao, K., Thompson, P.R., Hwang, Y., Marmorstein, R., Cole, P. A.  The structural basis of protein acetylation by the p300/CBP transcriptional coactivator.  Nature 451:846-850, 2008.  Pub Med Reference
     
  • Qiu, Q., Tarrant, M.K., Lee, S.H., Satyamurthy, A., Bose, R., Banjade, S., Pal, A., Bornmann, W.G., Lemmon, M.A., Cole, P.A., Leahy, D.J.  Mechanism of activation and inhibition of the HER4/ErbB4 kinase.  Structure 16:460-467, 2008.  Pub Med Reference
     
  • Mathivanan, S. et al.  Human Proteinpedia enables sharing of human protein data.  Nat. Biotechnol. 26:164-167, 2008.  Pub Med Reference
     
  • Levinson, N.M., Seeliger, M.A., Cole, P.A., Kuriyan, J.  Structural basis for the recognition of c-Src by its inactivator Csk.  Cell 134:124-134, 2008.  Pub Med Reference
     
  • Pickin, K.A., Chaudhury, S., Dancy, B.C.R., Gray, J.J., Cole, P.A.  Analysis of autophosphorylation of a protein kinase by expressed protein ligation.  J. Am. Chem. Soc. 130:5667-5669, 2008.  Pub Med Reference
  • Tang, Y., Holbert, M.A., Wurtele, H., Meeth, K., Rocha, W., Gharib, M., Jiang, E., Thibault, P., Verrauly, A., Cole, P.A., Marmorstein, R.  Fungal Rtt109/KAT11 histone acetyltransferase: an unexpected structural homolog of metazoan p300/CBP.  Nat. Struct. Mol. Biol. 15:738-745, 2008.  Pub Med Reference

  • Liu, Y., Dentin, R., Chen, D., Hedrick, S., Ravnskaer, K., Schenk, S., Milne, J., Meyers, D.J., Cole, P.A., Yates, J., Olefsky, J., Guarente, L., Montminy, M.  A fasting inducible acetylase/deacetylase switch modulates gluconeogenesis through activator-coactivator exchange.  Nature 456:269-273, 2008.  Pub Med Reference

  • Cole, P. A.  Chemical probes for histone-modifying enzymes.  Nat. Chem. Biol. 4, 590-597, 2008.  Pub Med Reference
     
  • Szewczuk, L.M., Tarrant, M.K., Sample, V., Drury, W.J. III, Zhang, J., Cole, P.A.  Analysis of serotonin N-acetyltransferase regulation in vitro and in live cells using protein semisynthesis.  Biochemistry 47:10407-10419, 2008.  Pub Med Reference
  •  
  • Wang, L., Tang, Y., Cole, P.A.*, Marmorstein, R.*  Structure and chemistry of the p300/CBP and Rtt109 histone acetyltransferases: Implications for histone acetyltransferase evolution and function.  Curr. Op. Struct. Biol. 18:741-747, 2008. (*co-corresponding author)  Pub Med Reference
  •  
  • Tarrant, M.K. and Cole, P.A.  The chemical biology of protein phosphorylation.  Ann. Rev. Biochem 78:797-825, 2009.  Pub Med Reference
  •  
  • Hansson, M.L., Popko-Scibor, A.E., Saint Just Ribiero, M., Dancy, B.M., Lindberg, M.L., Cole, P.A., Wallberg, A.E.  The transcriptional coactivator MAML1 regulates p300 autoacetylation and HAT activity.  Nucl. Acids Res. 37(9):2996-3006, 2009.  Pub Med Reference
  •  
  • Wang, D., Xia, X., Liu, Y., Oetting, A., Walker, R.L., Meltzer, P., Cole, P.A., Zhu, Y., Shi, Y.-B., Yen, P.M.  Negative regulation of TSHa target gene by thyroid hormone involves histone acetylation and corepressor complex dissociation.  Mol. Endocrinol. 23:600-609, 2009.  Pub Med Reference
  •  
  • Muratore, K.E., Seeliger, M.A., Wang, Z., Fomina, D., Neiswinger, J., Havranek, J.J., Baker, D., Kuriyan, J., Cole, P.A.  Comparative analysis of mutant tyrosine kinase chemical rescue.  Biochemistry 48:3378-3386, 2009.  Pub Med Reference
  •  
  • Qiu, C., Tarrant, M.K., Boronina, T., Longo, P.A., Kavran, J.M., Cole, R.N., Cole, P.A.*, Leahy, D.J.*  In vitro enzymatic characterization of near full length EGFR in activated and inhibited states.  Biochemistry 48:6624-6632, 2009. (*co-corresponding)  Pub Med Reference
  •  
  • Oussaief, L., Hippocrate, A., Ramirez, V., Rampanou, A., Zhang, W., Meyers, D., Cole, P., Khelifa, R., Joab, I.  PI3-K/Akt pathway targets acetylation of Smad3 through Smad3/CBP interaction; contribution to TGF-¾1-induced EBV reactivation.  J. Biol. Chem. 284:23912-23924, 2009.  Pub Med Reference
     
  • Karukurichi, K. R., Wang, L., Uzasci, L., Manlandro, C.M., Wang, Q., Cole, P.A.  Analysis of p300/CBP histone acetyltransferase regulation using circular permutation and semisynthesis.  J. Am. Chem. Soc. 132:1222-1223, 2010.  Pub Med Reference
     
  • Culhane, J., Wang, D., Yen, P.M., Cole, P.A.  Comparative analysis of small molecules and histone substrate analogs as LSD1 lysine demethylase inhibitors.  J. Am. Chem. Soc. 132:3164-3176, 2010.  Pub Med Reference
     
  • Mali, P., Chou, B.-K., Yen, J., Ye, Z., Zou, J., Dowey, S., Brodsky, R.A., Ohm, J.E., Yu, W., Baylin, S.B., Yusa, K., Bradley, A., Meyers, D.J., Mukherjee, C., Cole, P.A., Cheng, L.  Butyrate greatly enhances derivatization of human induced pluripotent stem cells by promoting epigenetic remodeling and the expression of pluripotency-associated genes.  Stem Cells 28:713-720, 2010.  Pub Med Reference
     
  • Bowers, E.M., Yan, G., Mukherjee, C., Orry, A., Wang, L., Holbert, M.A., Crump, N.T., Hazzalin, C.A., Liszczak, G., Yuan, H., Larocca, C., Saldanha, S.A., Abagyan, R., Sun, Y., Meyers, D.J., Marmorstein, R., Mahadevan, L.C., Alani, R.M., Cole, P.A.  Virtual ligand screening of the p300/CBP histone acetyltransferase: Identification of a selective small molecule inhibitor.  Chem. Biol. 17:471-482, 2010.  Pub Med Reference
     
  • Ahn, Y.H., Hwang, Y., Liu, H., Wang, X.J., Stephenson, K.K., Boronina, T.N., Cole, R.N., Dinkova-Kostova, A.T., Talalay, P., Cole, P.A.  Electrophilic tuning of the chemoprotective natural product sulforaphane.  Proc. Natl. Acad. Sci. USA 107:9590-9595, 2010.  Pub Med Reference
     
  • Huang, R., Martinez-Ferrando, I., Cole, P.A.  Enhanced interrogation: emerging strategies for cell signaling inhibition.  Nat. Struct. Mol. Biol. 17:646-649, 2010.  Pub Med Reference
     
  • Huang, R., Holbert, M.A., Tarrant, M.K., Curtet, S., Dancy, B.M., Dancy, B.C., Hwang, Y., Colquhoun, D.R., Tang, Y., Meeth, K., Marmorstein, R., Cole, R. N., Khochbin, S., Cole, P.A.  Site-specific introduction of an acetyl-lysine mimic into peptides and proteins by cysteine alkylation.  J. Am. Chem. Soc. 132:9986-9987, 2010.  Pub Med Reference
     
  • Reynoird, N., Schwartz, B.E., Delvecchio, M., Sadoul, K., Meyers, D., Mukherjee, C., Caron, C., Kimura, H., Rousseaux, S., Cole, P.A., Panne, D., French, C.A., Khochbin, S.  Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains.  EMBO J. 29:2943-2952, 2010.  Pub Med Reference
  •  
  • Min, S.-W., Cho, S.-H., Zhou, Y., Schroeder, S., Seeley, W.W., Huang, E.J., Shen, Y., Masliah, E., Mukherjee, C., Meyers, D., Cole, P.A., Ott, M., Gan, L.  Tau acetylation inhibits its degradation and contributes to tauopathy.  Neuron 67(6):953-966, 2010.  Pub Med Reference
  •  
  • Cerchietti, L.C., Hatzi, K., Caldas-Lopes, E., Yang, S.N., Figueroa, M.E., Morin, R.D., Hirst, M., Mendez, L., Shaknovich, R., Cole, P.A., Bhalla, K., Gascoyne, R.D., Marra, M., Chiosis, G., Melnick, A.  BCL6 repression of EP300 provides a basis for rational combinatorial therapy in diffuse large B cell lymphomas.  J. Clin. Invest. 120: 4569-4582, 2010.  Pub Med Reference
  •  
  • Bellows, M.L., Taylor, M.S., Shen, L., Cole, P.A., Fung, H., Siliciano, R.F., Floudas, C.A.  Discovery of entry inhibitors for HIV-1 via a new de novo protein design framework.  Biophys. J. 99(10):3445-3453, 2010.  Pub Med Reference
  •  
  • Barnett, B.P., Hwang, Y., Taylor, M., Kirchner, H., Pfluger, P.T., Bernard, V., Lin, Y.Y., Bowers, E.M., Mukherjee, C., Song, W.J., Longo, P.A., Leahy, D.J., Hussain, M.A., Tschöp, M., Boeke, J.D., Cole, P.A.  Glucose and weight control in mice with a designed ghrelin O-acyltransferase inhibitor.  Science 330:1689-1692, 2010.  Pub Med Reference
  •  
  • Taverna, S.D., Cole, P.A.  Drug discovery: Reader's block.  Nature 468:1050-1051, 2010.  Pub Med Reference
  •  
  • Karukurichi, K., Cole, P.A.  Probing the reaction coordinate of the p300/CBP histone acetyltransferase with bisubstrate analogs.  Bioorg. Chem. 39(1):24-47, 2011.  Pub Med Reference
  •  
  • Tang, Y., Holbert, M.A., Delgoshaie, N., Wurtele, H., Guillemette, B., Meeth, K., Yuan, H., Drogaris, P., Lee, E.-H., Durette, C., Thibault, P., Verreault, A., Cole, P.A., Marmorstein, R.  Structure of the Rtt109-AcCoA/Vps75 complex and implications for chaperone-mediated histone acetylation.  Structure 19:221-231, 2011.  Pub Med Reference
  •  
  • Blair, L.P., Avaritt, N.L., Huang, R., Cole, P.A., Taverna, S.D., Tackett, A.J.  MassSQUIRM: an assay for quantitative measurement of lysine demethylase activity.  Epigenetics 6(4):490-499, 2011.  Pub Med Reference
  •  
  • Collins, S.L., Black, K.E., Chan-Li, Y.B., Ahn, Y.-H., Cole, P.A., Powell, J.D., Horton, M.R.  Hyaluron fragments promote inflammation by down-regulating the anti-inflammatory A2a receptor.  Am. J. Resp. Phys. Cell Mol. Biol. 45:675-83, 2011.  Pub Med Reference         
  •  
  • Smirnova, N.A., Haskew-Layton, R.E., Basso, M., Hushpolian, D.M., Payappilly, J.B., Speer, R.E., Ahn, Y.-H., Rakhman, I., Cole, P.A., Pinto, J.T., Ratan, R.R., Gazaryan, I.G.  Development of Neh2-luciferase reporter and its application for high throughput screening and real-time monitoring of Nrf2 activators.  Chem. Biol. 18:752-766, 2011.  Pub Med Reference
  •  
  • Marek, R., Coelho, C.M., Sullivan, R.W., Baker-Andresen, D., Li, X., Ratnu, V., Dudley, K.J., Meyers, D., Mukherjee, C., Cole, P.A., Sah, P., Bredy, T.W.  Paradoxical enhancement of fear extinction memory and synaptic plasticity by inhibition of the histone acetyltransferase p300.  J. Neurosci. 31:7486-7451, 2011.  Pub Med Reference
  •  
  • Crump, N.T., Hazzalin, C.A., Bowers, E.M., Alani, R.M., Cole, P.A., Mahadevan, L.  Dynamic acetylation targeted to all lysine-4-trimethylated histone H3 is evolutionary conserved and mediated by p300/CBP.  Proc. Natl. Acad. Sci. USA 108:7814-7819, 2011.  Pub Med Reference
  •  
  • Xu, C.R., Cole, P.A., Meyers, D.J., Kornish, J., Dent, S., Zaret, K.S.  Pre-pattern and histone modifiers in a fate choice for liver and pancreas.  Science 332:963-966, 2011.  Pub Med Reference
  •  
  • Wang, L., Gural, A., Sun, X.-J., Zhao, X., Perna, F., Huang, H., Hatlen, M.A., Vu, L., Xu, H., Deblasio, T., Menendez, S., Voza, F., Cole, P.A., Melnick, A., Roeder, R.G., Nimer, S.D.  The leukemogenicity of AML1-ETO is dependent on site-specific lysine acetylation.  Science 333:765-769, 2011.  Pub Med Reference
  •  
  • Santer, F.R., Hoschele, P.P.S., Oh, S.J., Erb, H., Bouchal, J., Cavaretta, I.T., Meyers, D.J., Cole, P.A., Culig, Z.  Inhibition of the acetyltransferases p300 and CBP reveals a targetable function for p300 in the survival and invasion pathways of prostate cancer cell lines.  Mol. Cancer Ther. 10:1644-1655, 2011.  Pub Med Reference
     
  • Wang, Z., Longo, P.A., Tarrant, M.K., Kim, K., Head, S., Leahy, D.J., Cole, P.A. (2011) Mechanistic insights into the activation of oncogenic forms of the EGF receptor.  Nature Struct. Mol. Biol. 18, 1388-1393.  Pub Med Reference
     
  • Zhang, Y., Ahn, Y.-H., Benjamin, I.J., Honda, T., Hicks, R.J., Calabrese, V., Cole, P.A., Dinkova-Kostova, A. (2011) A novel role for sulfhydryl-reactive inducers of the KEAP1/NRF2/ARE pathway: HSF1-dependent upregulation of Hsp70.  Chem. Biol. 18, 1355-1361.  Pub Med Reference
     
  • Tarrant, M.K., Rho, H.-S., Xie, Z., Jiang, Y.L., Gross, C., Culhane, J.C., Yan, G., Qian, J., Ichikawa, Y., Matsuoka, T., Zachara, N., Etzkorn, F.A., Hart, G.W., Jeong, J.-S., Blackshaw, S., Zhu, H., Cole, P.A. (2012) Phosphorylation and O-GlcNAc regulation of CK2 kinase revealed through semisynthesis.  Nature Chem. Biol. 8, 262-269.  Pub Med Reference
     
  • Dancy, B.C., Ming, S.A., Papazyan, R., Jelinek, C.A., Majumdar, A., Sun, Y., Dancy, B.M., Drury, W.J., Cotter, R.J., Taverna, S.D., Cole, P.A. (2012) Azalysine analogs as probes for protein lysine deacetylation and demethylation.  J. Am. Chem. Soc. 134, 5138-5148.  Pub Med Reference
     
  • Wang, Y., Toh, H.C., Chow, P., Chung, A.Y., Meyers, D.J., Cole, P.A., Ooi, L.L., Lee, C.G. (2012) MicroRNA-224 is up-regulated in hepatocellular carcinoma through epigenetic mechanisms.  FASEB J. 26, 3032-3041.  Pub Med Reference
     
  • Francetic, T., May, M.L., Hamed, M., Mach, H., Meyers, D., Cole, P.A., Chem, J., Li, Q. (2012)�Regulation of Myf5 early enhancer by histone acetyltransferase p300 during stem cell differentiation.  Mol. Biol. 1, 1-7.  Pub Med Reference not available
     
  • Martinez-Ferrando, I., Chaerkady, R., Zhong, J., Molina, H., Kishore, H., Herbst, K., Dancy, B.M., Katju, V., Bose, R., Zhang, J., Pandey, A., Cole, P.A. (2012) Identification of targets of c-Src by chemical complementation and phosphoproteomics. Mol. Cell. Proteomics 11, 355-369.  Pub Med Reference
     
  • Wei, W., Coehlo, C., Li, X., Marek, R., Yan, S., Anderson, S., Meyers, D., Mukherjee, C., Sbardella, G., Castellano, S., Milite, C., Rotili, D., Mai, A., Cole, P.A. Sah, P., Kobor, M., Bredy, T. (2012) p300/CBP-associated factor selectively regulates the extinction of conditioned fear.  J. Neurosci. 32, 11930-11941.  Pub Med Reference
     
  • Taylor, M.S., Hwang, Y., Hsiao, P.-Y., Boeke, J.D., Cole, P.A. (2012) Ghrelin O-acetyltransferase assays and inhibition.  Meth. Enz. 514, 205-228.  Pub Med Reference
     
  • Dancy, B.M., Crump, N.T., Peterson, D.J., Mukherjee, C., Bowers, E.M., Ahn, Y.-H., Yoshida, M., Zhang, J., Mahadevan, L.C., Meyers, D.J., Boeke, J.D., Cole, P.A. (2012) Live cell studies of p300/CBP histone acetyltransferase activity and inhibition.  ChemBioChem 13, 2113-2121.  Pub Med Reference
     
  • Teubner, B.J.W., Garretson, J.T., Hwang, Y., Cole, P.A., Bartness, T.J. (2013) Inhibition of ghrelin O-acetyltransferase attenuates food deprivation-induced increases in ingestive behavior.  Hormones and Behavior 63, 667-673.  Pub Med Reference
     
  • Newman, R., Hu, J., Rho, H.-S., Xie, Z., Woodard, C., Neiswinger, J., Cooper, C., Shirley, M., Clark, H., Hu, S., Hwang, W., Jeong, J.S., Wu, G., Lin, J., Gao, X., Ni, Q., Goel, R., Xia, S., Ji, H., Dalby, K., Birnbaum, M., Cole, P.A., Knapp, S., Ryazanov, A., Zack, D., Blackshaw, S., Pawson, T., Gingras, A.-C., desiderio, S., Pandey, A., Turk, B., Zhang, J., Zhu, H., Qian, J. (2013) Construction of activity based networks.  Mol. Syst. Biol. 9, 655.  Pub Med Reference
     
  • Yan, G., Eller, M.S., Elm, C., Larocca, C., Dancy, B., Bowers, E., Meyers, D., Lareau, L., Cole, P.A.*, Taverna, S.D.*, Alani, R.M.* (2013) Selective inhibition of p300/CBP histone acetyltrasferase activity reveals its roles in tumor proliferation, cell cycle progression, and DNA damage response.  J. Inv. Derm. in press. (*co-corresponding author)  Pub Med Reference
     
  • Liu, Y., Wang, L., Predina, J., Han, R., Beier, U.H., Bhatti, T.R., Akimova, T., Singhal, S., Brindle, P.K., Cole, P.A., Albelda, S.M., Hancock, W.W. (2013) Inhibition of p300 impairs Foxp3+ T-regulatory cell function and promotes anti-tumor immunity.  Nature Medicine in press. Pub Med Reference
     
  • Bolduc, D.M., Rahdar, M., Tu-Sekine, B., Sivakumaren, S.C., Raben, D.M., Amzel, L.M., Devreotes, P.N., Gabelli, S.B., Cole, P.A. (2013) Phosphorylation-mediated PTEN conformational closure and deactivation revealed with protein semisynthesis.  eLife 2, e00691.  Pub Med Reference

Other graduate programs in which Dr. Cole participates:


BCMB Program
Chemistry-Biology Interface Program (CBI)
Program in Molecular Biophysics

Staff

Research Associates

Dr. Shridhar Bhatshri@jhu.edu410-502-4803
Dr. You Sang Hwangyhwang4@jhmi.edu410-614-0322
Dr. David Meyersdmeyers7@jhmi.edu410-502-4804
Qiang Niqni1@jhmi.edu410-614-2892
Dr. Stefani Thomassthomas92@jhmi.edu410-287-6362

Post-Doctoral Fellows

Dr. Nam Chukchu7@jhmi.edu410-614-0322
Dr. Jayanta Dasjdas3@jhmi.edu410-502-4804
Dr. Jay Kalinjkalin1@jhmi.edu410-614-0322
Dr. Yun Wangywang144@jhmi.edu410-614-0322
Dr. Zhihong Wangzwang37@jhmi.edu410-614-0322
Dr. Beth Zucconibzuccon1@jhmi.edu410-614-0322

Graduate Students

Zan Chenzchen30@jhmi.edu410-614-0322
Dawn Haywarddhaywar5@jhmi.edu410-614-0322
Sam Henagershenage1@jhu.edu410-614-0322
Robert Hsiaophsiao@jhmi.edu410-614-0322

Martin Taylor

mstaylor@jhmi.edu

410-955-2474

Techncian
   

Sr. Administrative Coordinator

Robin Hart

rhart@jhmi.edu

410-614-8849



 

 
 
 
 
 
 

© The Johns Hopkins University, The Johns Hopkins Hospital, and Johns Hopkins Health System. All rights reserved.

Privacy Policy and Disclaimer