TCNP Protocols

BOEKE Lab Protocols

• Yeast high efficiency transformation - dSLAM (PDF)
• Yeast sporulation - dSLAM (PDF)
  

PANDEY Lab Protocols

• Stable isotope labeling with amino acids in cell culture (SILAC) protocol for quantitation of protein dynamics (PDF)
• 18O - labeling trypsin digest protocol for relative quantitation by mass spectrometry

COTTER Lab Protocols

• Quantification of lysine acetylation (PDF)
• N-terminal derivatization for de novo sequencing (PDF)
• Determination of ubiquitylation sites (PDF)
• Determination of SUMOylation sites (PDF)

COLE Lab Protocols

• Chemical synthesis of a novel coenzyme A-based affinity probe (PDF)

MELUH Protocols

• Indirect Immunofluorescence for Yeast (PDF)
• DAPI Staining Yeast (PDF)
• Chromatin Immunoprecipitation (ChIP) for Yeast (PDF)
  

RANDO Protocols

• Mononucleosome Chromatin Immunoprecipitation for Yeast (PDF)

02/02/2009