Skip Navigation
  Search Menu
Find an Expert

 

Andrew S. McCallion, Ph.D.

Photo of Dr. Andrew S. McCallion, Ph.D.

Assistant Director, Human Genetics Graduate Program

Associate Professor of Molecular and Comparative Pathobiology

Male

Research Interests: Genetics basis of congenital malformations; Genetics of neurological, neuropsychiatric and neural-crest disorders; Transcriptional regulation in development and disease ...read more

Background

Dr. Andrew McCallion is an associate professor of molecular and comparative pathobiology at the Johns Hopkins University School of Medicine. His research focuses on applying functional genetics to human development and disease. Dr. McCallion was part of a research team at Johns Hopkins that identified the two genes that cause Hirschsprung disease, an inherited intestinal disorder.

His research focuses on making the connection between gene sequence (and variation therein) and phenotype through the integrated use of contemporary genomic strategies and model systems (mouse, zebrafish and cell culture).

Dr. McCallion received his B.Sc. in genetics from The Queen's University of Belfast. He earned his Ph.D. in genetics from the University of Glasgow. He completed postdoctoral training at Case Western Reserve University Medical School.

Prior to joining Johns Hopkins, Dr. McCallion was a project leader and staff scientist at Neuropa Ltd. (UK), a biotech startup focused on drug target development for neurodegenerative disorders.

He is a member of the International Mammalian Genome Society, American Society of Human Genetics and Federation of American Societies for Experimental Biology. He serves on the editorial board of Genome Research, and is a Faculty of 1000 faculty member in genomics and genetics.

...read more

Titles

  • Assistant Director, Human Genetics Graduate Program
  • Associate Professor of Molecular and Comparative Pathobiology
  • Associate Professor of Medicine
  • Associate Professor of Pediatrics

Departments / Divisions

Centers & Institutes

Research & Publications

Research Summary

My group studies how transcriptional regulatory control is encrypted in genomic sequence. We seek to define the cellular contexts within which noncoding variants mediate their effects and how such variation in regulatory sequences may contribute to phenotype variation and disease risk/presentation. In this work my group employs cutting edge genomic and functional genetic approaches in mice, zebrafish and in vitro, integrating them with computational biology.

Regulatory sequences underlie the cellular diversity that arises during human development, and how cells respond to environmental and genetic insult. Regulatory mutations underlie an array of human diseases. They play a significant role in disease susceptibility and they form the basis of cellular response to insult, aging and stress.

Efforts in my lab are currently directed at developing cell-type dependent regulatory sequence catalogs and applying them in human population-based studies to predict, identify and validate likely functional variation that associates with disease. In some of our recent work we have generated large catalogs of putative enhancers in various cell types using ChIP-seq and ATAC-seq (melanoctytes, dopaminergic neurons [ventral midbrain, forebrain, olfactory bulb]). Further begun to explore how these data can be used to learn the vocabularies of cell-dependent control inform our understanding of functional non-coding variation and the molecular mechanisms of transcriptional control.  Our emerging work has begun to integrate these analyses with studies of transcriptional heterogeneity within cell-types, using single cell RNA-seq to explore specific neuronal populations and define their Gene Regulatory Networks (GRNs).

Discernment of disease-relevant cell populations, pertinent biological states and stages, is essential for comprehensive functional investigation of their contribution to risk. We have begun to address this question by leveraging stratified LD [Linkage Disequilibrium] score regression (S-LDSC) to partition heritability from GWAS summary statistics to sets of cell-dependent biological signatures in order to identify cell types relevant to disease. We are evaluating common human traits using ATAC-seq data from a wide array of purified mouse cell populations. Our efforts to date have already revealed that OCR signatures from specific neuron sub-populations are highly enriched for schizophrenia heritability.

Collectively, our work sets a powerful precedent for our continued study of a wide assortment of common human neurological and neuropsychiatric phenotypes.

Lab

Gene regulation is the framework on which vertebrate cellular diversity is built. The substantial cellular diversity that characterizes complex integrated cell populations, such as the human central nervous system, must therefore require immense regulatory complexity. Similarly, the cells comprising the embryonic neural crest, a population that contributes craniofacial cartilage and bone, pigment cells of the skin and hair, neuroendocrine cells and the entire peripheral nervous system to the vertebrate embryo, must face similar challenges in choosing the correct fate. These cells go awry in a wide array of human disorders like Parkinson's disease, Hirschsprung disease, psychiatric disorders and melanoma, and comprise the focus of our efforts.

Although regulatory control acts at many levels, we focus on the roles played by cis-regulatory elements (REs) in controlling the timing, location and levels of gene activation (transcription). However, the biological relevance of non-coding sequences cannot be inferred by examination of sequence alone. Perhaps the most commonly used indicator of non-coding REs is evolutionary sequence conservation. Although conservation can uncover functionally constrained sequences, it cannot predict biological function, and regulatory function is not always confined to conserved sequences. At its simplest level, regulatory instructions are inscribed in transcription factor binding sites (TFBS) within REs. Yet, while many TFBS have been identified, TFBS combinations predictive of specific regulatory control have not yet emerged for vertebrates. We posit that motif combinations accounting for tissue-specific regulatory control can be identified in REs of genes expressed in those cell types. Our immediate goal is to begin to identify TFBS combinations that can predict REs with cell-specific biological control—a first step in developing true regulatory lexicons.

As a functional genetic laboratory, we develop and implement assays to rapidly determine the biological relevance of sequence elements within the human genome and the pathological relevance of variation therein. In recent years, we have developed a highly efficient reporter transgene system in zebrafish that can accurately evaluate the regulatory control of mammalian sequences, enabling characterization of reporter expression during development at a fraction of the cost of similar analyses in mice. We employ a range of strategies in model systems (zebrafish and mice), as well as analyses in the human population, to illuminate the genetic basis of disease processes. Our long-term objective is to use these approaches in contributing to improved diagnostic, prognostic and ultimately therapeutic strategies in patient care.

If you are interested in learning more about the work we do or would like to inquire about positions available within the lab, please contact Dr. McCallion (andy@jhmi.edu).

Lab Website: Andrew McCallion Laboratory
Core Facility: Phenotyping (and Pathology) Core (Phenocore)

Selected Publications

View all on Pubmed

Fisher S, Grice EA, Vinton RM, Bessling SL, McCallion AS. (2006) Conservation of RET Regulatory Function from Human to Zebrafish Without Sequence Similarity. Science. 312, 276-279.

David Gorkin, Dongwon Lee, Xylena Reed, Christopher Fletez-Brant, Stacie K. Loftus, Michael A. Beer, William J. Pavan and McCallion, A.S. Integration of ChIP-seq and Machine Learning Reveals Enhancers and a Predictive Regulatory Sequence Vocabulary in Melanocytes. Genome Res. 2012;22(11):2290-301.

Lee D, Gorkin DU, Baker M, Strober BJ, Asoni AL, McCallion, A.S.‚† and Michael A. Beer‚† A method to predict the impact of regulatory variants from DNA sequence. Nature Genetics. 2015 Aug;47(8):955-61. ‚†, Co-corresponding authors

Hook PW, McClymont SA, Cannon GH, Law WD, Morton AJ, Goff LA‚, McCallion AS‚. Single-cell RNA-seq of dopaminergic neurons informs candidate gene selection for sporadic Parkinson's disease. American Journal of Human Genetics 2018 Mar 1;102(3):427-446. doi: 10.1016/j.ajhg.2018.02.001 (Cotterman Award winner - Outstanding AJHG paper of 2018)

McClymont SA, Hook PW, Briceno NJ, Reed X, Soto AI, Law WD, Ross OA, Visel A, Pennacchio L, Beer MA, McCallion AS. Parkinson-associated SNCA intronic enhancer variants revealed by open chromatin in mouse dopamine neurons. American Journal of Human Genetics - In press; https://www.biorxiv.org/content/early/2018/07/08/364257

Contact for Research Inquiries

Johns Hopkins University
733 N. Broadway
Institute for Genetic Medicine
Baltimore, MD 21205 map

Academic Affiliations & Courses

Graduate Program Affiliation

Preceptor-Predoctoral Training Program in Human Genetics

Graduate Student/Fellow Training – My lab is built on the hard work of many bright undergraduates, graduates and fellows. I am the Assistant Director of the Human Genetics Graduate Program and I serve on the Human Genetics Executive Committee. I am also an active participant in the graduate programs of Molecular Biology and Genetics, Cellular and Molecular Medicine and Biochemistry, Cellular and Molecular Biology. I serve on many comprehensive examination and thesis advisory committees.

I presently mentor two students in the Human Genetics graduate program. Over the last 14 years, I have mentored 12 graduate students (10 PhD; 2 MA); I have trained five post-doc/fellows, a host of undergraduate students and provided extensive research opportunity to ≥10 graduate technicians seeking lab experience prior to medical/graduate school.

Courses and Syllabi

  • Molecular Mechanisms of Disease (Director) (ME 710.702)
  • Advanced Topics in Human Genetics (Lecturer) (ME 710.700)
  • Phenotyping for Functional Genetics (Lecturer) (ME 680.712)
  • Short Course in Medical and Experimental Mammalian Genetics (Lecturer)

Activities & Honors

Honors

  • Faculty of 1000, Genomics and Genetics

Memberships

  • American Society of Human Genetics
    Member
  • Federation of American Societies for Experimental Biology
    Member
  • International Mammalian Genome Society
    Member

Professional Activities

  • Editorial board, Genome Research, 2006
  • Rodent Advisory Committee, Johns Hopkins University, 0
  • Rodent Phenotyping CORE committee, Johns Hopkins University, 0
Is this you? Edit Profile