The Timp lab’s focus is on the development and application of sequencing technologies to gain a deeper understanding of biology and a more accurate set of clinical tools for human disease. Timp’s research integrates the principles of biophysics, molecular biology, and computational biology to create new tools for exploring the epigenomes and genomes of different lifeforms, ranging in size from the flu virus to hummingbirds to California redwoods. Based on the knowledge gained from these studies, Timp and his lab apply their toolsets to clinical samples for the diagnosis, surveillance and treatment of human disease. Recent projects in Timp’s lab include new sequencing methods to diagnose infectious disease, new methods to characterize RNA biology, and examining single molecule epigenetics of cancer.
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J. T. Simpson, R. E. Workman, P. C. Zuzarte, M. David, L. J. Dursi, and W. Timp, “Detecting DNA cytosine methylation using nanopore sequencing,” Nature Methods, vol. 14, no. 4, pp. 407–410, Apr. 2017
R. E. Workman*, A. D. Tang*, P. S. Tang*, M. Jain*, J. R. Tyson*, R. Razaghi*, P. C. Zuzarte, T. Gilpatrick, A. Payne, J. Quick, N. Sadowski, N. Holmes, J. G. de Jesus, K. L. Jones, C. M. Soulette, T. P. Snutch, N. Loman, B. Paten, M. Loose, J. T. Simpson, H. E. Olsen, A. N. Brooks, M. Akeson§, and W. Timp, “Nanopore native RNA sequencing of a human poly(A) transcriptome,” Nature Methods, pp. 1–9, Nov. 2019
T. Gilpatrick, I. Lee, J. E. Graham, E. Raimondeau, R. Bowen, A. Heron, B. Downs, S. Sukumar, F. J. Sedlazeck, and W. Timp, “Targeted nanopore sequencing with Cas9-guided adapter ligation,” Nature Biotechnology, Feb. 2020
I. Lee, R. Razaghi, T. Gilpatrick, M. Molnar, A. Gershman, N. Sadowski, F. J. Sedlazeck, K. D. Hansen, J. T. Simpson, and W. Timp, “Simultaneous profiling of chromatin accessibility and methylation on human cell lines with nanopore sequencing,” Nature Methods, vol. 17, no. 12, pp. 1191–1199, Dec. 2020
P. M. Thielen*, S. Wohl*, T. Mehoke, S. Ramakrishnan, M. Kirsche, O. Falade-Nwulia, N. S. Trovão, A. Ernlund, C. Howser, N. Sadowski, C. P. Morris, M. Hopkins, M. Schwartz, Y. Fan, V. Gniazdowski, J. Lessler, L. Sauer, M. C. Schatz, J. D. Evans, S. C. Ray, W. Timp, and H. H. Mostafa, “Genomic diversity of SARS-CoV-2 during early introduction into the Baltimore–Washington metropolitan area,” JCI Insight, vol. 6, no. 6, Mar. 2021.