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Tzyy-Choou Wu, M.D., M.P.H., Ph.D.

Headshot of Tzyy-Choou Wu
  • Director, Gynecologic Pathology Division
  • Professor of Pathology

Languages: English, Chinese, Taiwanese


Cervical Cancer, Head and Neck Cancers, Pathology

Research Interests

CD8+ T lymphocytes; cervical cancer; vaccination; human papillomavirus (HPV); HPV vaccine; cellular pathology; molecular pathology; T cell biology; tumor immunology; HPV immunology more


The Johns Hopkins Hospital (Main Entrance)

1800 Orleans St.
Sheikh Zayed Tower
Baltimore, MD 21287 map


Dr. Tzyy-Choou Wu is a professor of pathology, oncology, and obstetrics and gynecology at the Johns Hopkins University School of Medicine. He holds a joint appointment in the Bloomberg School of Public Health Department of Molecular Microbiology and Immunology.

Dr. Wu’s clinical interests include gynecological pathology, cervical cancer, head and neck cancers, and the molecular diagnosis of viral infections.

He and Dr. Chien-Fu Hung also oversee the Cervical Cancer Research Lab, which is working to develop vaccines and immunotherapeutic strategies to prevent and treat human papillomavirus (HPV)-associated cervical cancer.

Dr. Wu earned his M.D. at National Taiwan University College of Medicine before earning both his Ph.D. in molecular virology and his M.P.H. in epidemiology at the Johns Hopkins Bloomberg School of Public Health. He completed a residency in anatomic pathology and a fellowship in gynecologic pathology at Johns Hopkins. He joined the Johns Hopkins faculty as an assistant professor in 1995.

Dr. Wu has published more than 260 academic journal articles, written scores of editorials, reviews and book chapters, and delivered many invited lectures. He also holds a number of patents.

He serves on the editorial boards of publications that include the American Journal of Pathology, International Journal of Gynecological Pathology, Gene Therapy and Cancer Research.

Dr. Wu is a member of professional organizations that include the American Society for Clinical Investigation, American Association for Cancer Research and International Society of Gynecological Pathologists. Among his many activities, Dr. Wu serves as director of the Cervical Cancer Steering Committee and as a member of the Kimmel Comprehensive Cancer Center Research Council. more


  • Director, Gynecologic Pathology Division
  • Director, Cervical Cancer Research Lab
  • Professor of Pathology
  • Professor of Gynecology and Obstetrics
  • Professor of Oncology

Departments / Divisions

Centers & Institutes



  • MD; National Taiwan University Medical School (1982)


  • Pathology; Johns Hopkins University School of Medicine (1992)


  • Pathology; Johns Hopkins University School of Medicine (1995)

Board Certifications

  • American Board of Pathology (Anatomic Pathology) (1992)

Research & Publications

Research Summary

Dr. Wu’s research focuses on human papillomavirus (HPV) vaccine development.

He has created a unique preclinical murine tumor model that expresses HPV-16 oncogenic proteins (E6 and E7) and simulates specific molecular events in the progression of HPV+ precancerous lesions (CIN 3) to invasive cancer.

This model has been widely used and tested by researchers worldwide for HPV vaccine development.

Dr. Wu has focused on identifying immunotherapeutic and vaccine approaches to enhance antigen processing and presentation by dendritic cells. These include intracellular targeting and spreading strategies aimed at preventing and treating cervical lesions and cancers. Intracellular targeting directs antigens to different subcellular locations to enhance antigen processing and presentation.

Intercellular spreading helps antigens distribute to neighboring cells by taking advantage of unique intercellular transport properties that allows for an increase in the amount of antigen presented to effector cells.

Dr. Wu has created an innovative approach that combines both antigen-specific immunotherapy and anti-angiogenesis to treat HPV E7-expressing tumors.

The impressive preclinical data derived from these studies have led to several clinical trials that are either currently under way or will soon commence. The continued development of these strategies will facilitate the development of vaccines that generate a potent immune response and antitumor effect against cervical cancer.

Dr. Wu is also actively involved with investigating mechanisms of immune evasion of tumors, identifying new tumor-specific antigens and applying vaccine strategies to other cancer systems with characterized tumor-specific antigens.


The Cervical Cancer Research Lab of Drs. T-C Wu and Chien-Fu Hung has a multi-part mission to:

  • Create the most effective cervical cancer vaccine for the prevention and treatment of human papillomavirus-associated malignancies
  • Extend the application of the principles and strategies that we use for HPV vaccine development in order to create vaccines that target other cancers with defined tumor antigens
  • Develop innovative immunological assays for human papillomavirus vaccine clinical trials
  • Serve as an environment for the education of research technicians, graduate students and undergraduate students

Lab Website: Cervical Cancer Research Lab

Selected Publications

View all on PubMed

T.-C. Wu, F.G. Guarnieri, K.F. Staveley-O'Carroll, R.P. Viscidi, H.I. Levitsky, L. Hedrick, K.R. Cho, J.T. August, and D.M. Pardoll (1995) Engineering a novel pathway for MHC Class II Presentation of Antigens. Proc. Natl. Acad. Sci. 92: 11671-11675.

K.-Y. Lin, F. G. Guarnieri, K. F. Staveley-O'Carroll, H. I. Levitsky, J. T. August, D. M. Pardoll and T.-C. Wu (1996) Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen. Cancer Research 56:21-26.

C.-H. Chen, T.-L. Wang, C.-F. Hung, Y. Yang, H. Chen, R. A. Young, D. M. Pardoll and T.-C. Wu (2000) Enhancement of DNA vaccine potency by linkage of antigen gene to an HSP70 gene. Cancer Research, 60:1035-1042.

W.-F. Cheng, C.-F. Hung, C.-Y. Chai, K.-F. Hsu, L. He, M. Ling and T.-C. Wu (2001) Tumor-specific immunity and antiangiogenesis generated by a DNA vaccine encoding calreticulin linked to a tumor antigen. J. Clin. Invest. 108: 669-78.

T. W. Kim, C.-F. Hung, M. Ling, J. Juang, L. He, J. M. Hardwick, S. Kumar, and T.-C. Wu (2003) Enhancing DNA vaccine potency by co-administration of DNA encoding anti-apoptotic proteins. J. Clin. Invest. 112: 109-117.

C. L. Trimble, S. Peng, F. Kos, P. Gravitt, R. Viscidi, E. Sugar, D. Pardoll, T.-C. Wu (2009) A phase I trial of a HPV DNA vaccine for HPV16+ cervical intraepithelial neoplasia 2/3. Clinical Cancer Research 15(1):361-7.

K. H. Noh, B. W. Kim, K.-H. Song, H. Cho, Y.-H. Lee, J. H. Kim, H. Cho, J.-Y. Chung, J.-H. Kim, S. M. Hewitt, S.-Y. Seong, C.-P. Mao, T-C Wu* and T. W. Kim* (2012) Nanog signaling in cancer promotes stem-like phenotype and immune evasion. J. Clin. Invest. 122: 4077-4093 * Co-corresponding authors.

T.H. Kang,CP Mao, SY Lee, A Chen, JH Lee, T.W. Kim, R Alvarez, RB Roden, D Pardoll, CF Hung, T.-C. Wu (2013). Chemotherapy acts as an adjuvant to convert the tumor microenvironment into a highly permissive state for vaccination-induced antitumor immunity. Cancer Research. 73(8);2493-2504.

L. Maldonado, JE Teague, MP Morrow, I. Jotova , T.-C. Wu, C. Wang, C. Desmarais, JD Boyer, B. Tycko, HS Robins, RA Clark, CL Trimble. (2014) Intramuscular therapeutic vaccination targeting HPV16 induces T cell responses that localize in mucosal lesions. Sci Transl Med. 29 (6) 221.

Y Sun, S Peng, J Qiu, J Miao, B Yang, J Jeang, CF Hung, T.-C. Wu (2015) Intravaginal HPV DNA vaccination with electroporation induces local CD8+ T-cell immune responses and antitumor effects against cervicovaginal tumors. Gene Therapy advance online publication. 22 (7): 528-535​


DNA Vaccine Enhancement with MHC Class II Activators
Patent # US9085638 B2 | 2015

RNA Interference That Blocks Expression of Pro-Apoptotic Proteins Potentiates Immunity Induced by DNA and Transfected Dendritic Cell Vaccines
Patent # US9011866 B2 | 2015

Nucleic Acid Immunogenic Compositions Encoding Hsp-antigen Chimera
Patent # CA2388045 C | 2014

Superior Molecular Vaccine Linking the Translocation Domain of a Bacterial Toxin to an Antigen
Patent # US8128922 B2 | 2012

Molecular Vaccine Linking an Endoplasmic Reticulum Chaperone Polypeptide to an Antigen
Patent # US8007781 B2 | 2011

Contact for Research Inquiries

The Johns Hopkins University
School of Medicine
Cancer Research Building II, Room 309
Baltimore, MD 21205 map

Activities & Honors


  • Outstanding Pathologist Award, International Association of Chinese Pathologists, 2017
  • Scientific Award, the Chinese American Medical Society, 2008
  • Outstanding Service Award, Society of Chinese Bioscientists in America (DC Chapter), 2000
  • Physician Scientist Award, the Passano Foundation, 1995
  • Binford-Dammin Award, Binford-Dammin Society of the International Academy of Pathology, 1994
  • The Delta Omega Honorary Society Alpha Chapter, School of Hygiene & Public Health, Johns Hopkins University, 1989
  • Frederik B. Bang Award, School of Hygiene and Public Health, Johns Hopkins University, 1987
  • Hampil Fellowship, School of Hygiene and Public Health, Johns Hopkins University, 1986
  • Dr. Shu Yeh Scholarship, National Taiwan University, 1979


  • Society for Clinical Investigation
  • Association of American Physicians

    Dr. Wu is an elected member of the Association of American Physicians.

  • Academician, Academia Sinica
  • Fellow, American Society for Microbiology

Professional Activities

  • Member, Research Committee of JHU Women’s Health Initiatives, 1997
  • President, Society of Chinese Bioscientists in America (DC Chapter), 1998 - 1999
  • Director, Vaccine Immunology Basic Research Center, 2002 - 2006
  • Director, Cervical Cancer Steering Committee, 2002
  • Member, Research Council of the Sidney Kimmel Comprehensive Cancer Center at Hopkins, 2002
  • President, International Association of Chinese Pathologists, 2003 - 2005
  • Associate Editor, Journal of Biomedical Science, 2005
  • President, Binford-Dammin Infectious Disease Society of the International Academy of Pathology, 2006 - 2007
  • Co-Executive Director, Society of Chinese Bioscientists in America (SCBA), 2010 - 2015
  • Associate Editor, Cell and Bioscience, 2010

Patient Ratings & Comments

The Patient Rating score is an average of all responses to physician related questions on the national CG-CAHPS Medical Practice patient experience survey through Press Ganey. Responses are measured on a scale of 1 to 5, with 5 being the best score. Comments are also gathered from our CG-CAHPS Medical Practice Survey through Press Ganey and displayed in their entirety. Patients are de-identified for confidentiality and patient privacy.

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