The focus of the research in the Reddy lab is to begin to understand how the nuclear periphery and other subcompartments contribute to general nuclear architecture and to specific gene regulation. Understanding the cell biology of genomes and how nuclear architecture controls gene expression is necessary to truly understand biological processes such as development and disease.
Although sequencing of the genome and comparative genome analysis have yielded insights into the regulation and dis-regulation of genetic information, these efforts shed little light into how genomes actually work in vivo. The impact of architectural and cellular organization of genomes on gene activity is a next step to unlocking genetic and epigenetic mechanisms in development and disease. Specifically, they are trying to understand how genes are regulated at the nuclear periphery, decipher how genes are localized (or “addressed”) to specific nuclear compartments and, finally, determine how these processes are utilized in development and corrupted in disease.
Reddy KL, Singh HS (2008). “Using molecular tethering to analyze the role of nuclear compartmentalization in the regulation of mammalian gene activity.” Methods. 2008 Jul;45(3):242-51. doi: 10.1016/j.ymeth.2008.06.013. Epub 2008 Jul 9
Reddy KL, Johnson KJ, and Singh HS. (2008) Signaling and transcriptional control coordinating V(D)J recombination and B cell development” "V(D)J Recombination", edited by Dr. Ferrier, Landes Bioscience
Reddy KL, Schlimgen R, Singh HS, Krangel M. (2008).“The Role of Nuclear Localization in Regulating Tcrb Recombination and Allelic Exclusion.” Nature Immunology.
Reddy, KL, Zullo, JM, Bertolino E, Singh, HS. (2008) “Mammalian gene repression mediated by inducible repositioning to the nuclear membrane.” Nature, 452(7184): 243-7
Reynaud D, DeMarco I, Reddy KL, Schjerven H, Bertolino E, Smale ST, Winandy S, Singh HS (2008) “Key features of pro-B cells are specifically regulated by Ikaros.” Nature Immunology.
Bertolino E, Reddy K, Medina KL, Parganas E, Ihle J, and Singh H (2005). “Regulation of interleukin 7-dependent immunoglobulin heavy-chain variable gene rearrangements by transcription factor STAT5.” Nature Immunology 6, 836-843.
Medina, KL, Pongubala, JMR, Reddy KL, Lancki DW, DeKoter R, Kieslinger M, Grosschedl R, Singh H. (2004). “Assembling a Gene Regulatory Network for Specification of the B Cell Fate.” Developmental Cell 7, 607-617.