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Hui Zhang, M.S., Ph.D.

Headshot of Hui Zhang
  • Director of Mass Spectrometry Core Facility, Center for Biomarker Discovery and Translation, Johns Hopkins University
  • Professor of Pathology

Research Interests

Proteomics, Protein modifications and functions; Proteome scale; Glycoproteins ...read more

Background

Dr. Hui Zhang is a professor of pathology who specializes in proteomics with particular emphasis on protein modifications. She is the director of the Mass Spectrometry Core Facility, Center for Biomarker Discovery and Translation. Dr. Zhang studies protein modifications on the proteome scale and the effects of protein modifications on protein functions and diseases. For the past few years, her laboratory has developed several novel glycoproteomic and glycomic technologies to study structures of cell surface glycoproteins and secreted glycoproteins. Currently, researchers in her group focus on understanding the functions of protein modifications in biology and human diseases.

...read more

Titles

  • Director of Mass Spectrometry Core Facility, Center for Biomarker Discovery and Translation, Johns Hopkins University
  • Professor of Pathology
  • Associate Professor of Oncology
  • Joint Appointment in Urology

Departments / Divisions

Centers & Institutes

Education

Degrees

  • B.S.; Beijing University (China) (1989)
  • M.S.; Beijing University (China) (1992)
  • Ph.D.; University of Pennsylvania (Pennsylvania) (1999)

Additional Training

  • Institute for Systems Biology, Seattle, WA, 2003, Protein Chemistry

Research & Publications

Research Summary

Dr. Zhang studies protein modification on the proteome scale and the effects of modification on protein function and disease progression. For the past few years, her research has focused on the development of high-throughput technologies to isolate and identify two of the most abundant protein modifications - phosphorylation and glycosylation.

One technology enables capturing and identification of modified peptides using affinity chromatography. To do so, she has developed a group of antibodies reactive against a variety of protein modification sites, such as phosphorylation, nitration, acetylation, and substrates of a specific modification enzyme, etc. Among those, phospho-specific antibodies have enabled isolation of a large number of phosphorylated peptides that can be subsequently identified by tandem mass spectrometry.

A second technology enables capturing glycopeptides using solid phase extraction, which has become a powerful tool to analyze glycoproteins on cell surface and in body fluids. Thus far, thousands of novel glycosylation sites have been identified from different tissues using this novel glycopeptide capture technology; this significantly expends the limited number of glycosylation sites experimentally identified prior to the new technology.

These methods are highly sensitive, holding a strong promise for discovering low abundance disease marker proteins in tissue, plasma or other body fluids.

Currently, Dr. Zhang is applying these proteomics technologies to determine protein modifications associated with cancer, which well help for early detection and improved monitoring of therapeutic effects. She is also developing novel methods to study protein modifications that will have major implications for a wide range of health issues.

Technology Expertise Keywords

Proteomics; mass spectrometry; glycoproteimics; glycomics; phosphoproteomics

Selected Publications

Zhang H, Liu T, Zhang Z, Payne SH, Zhang B, McDermott JE, Zhou J, Petyuk VA, Chen L, Ray D, Sun S, Yang F, Chen L, Wang J, Shah P, Cha S-W, Aiyetan P, Woo S, Tian Y, Gritsenko MA, Choi C, Monroe ME, Thomas S, Moore RJ, ,Yu K-H, Tabb DL, Fenyö D, Bafna V, Wang Y, Rodriguez H, Boja ES, Hiltke T, Rivers RC, Sokoll L, Zhu H, Shih I-M, Pandey A, Zhang B, Snyder MP, Levine DA, Smith RD, Chan DW, Rodland KD, and the CPTAC investigators. Deep proteogenomic characterization of human ovarian cancer. Cell. 2016; 166: 755-765.

Sun S, Shah P, Toghi Eshghi S, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti NU, Zhang Z, Chan DW, Zhang H*. Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nature Biotechnology. 2016; 34: 84-88.

Shah P, Wang X, Yang W, Toghi Eshghi S, Sun S, Hoti N, Pasay J, Rubin A, Zhang H*. Integrated proteomic and glycoproteomic analyses of prostate cancer cells reveals glycoprotein alteration in protein abundance and glycosylation. Molecular & Cellular Proteomics. 2015; 14: 2753-2763.

Toghi Eshghi S, Shah P, Yang W, Li X, Zhang H*. GPQuest: A Spectral Library Matching Algorithm for Site-Specific Assignment of Spectra from Tandem Mass Spectrometric Analysis of Intact Glycopeptides. Analytical Chemistry. 2015; 87: 5181-5188.

Zhang, H., Li, X. J., Martin, D. B., and Aebersold, R. Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nature Biotechnology (2003) 21:660.

Patents

Biomarkers for Prostate Cancer.
Patent # 8,603,734 | 2007

Production of Motif-Specific and Context-Independent Antibodies Using Peptide Libraries as Antigens
Patent # 7,259,022 | 2001

Immunoaffinity Isolation of Modified Peptides From Complex Mixtures
Patent # 7,198,896 | 2002

Methods for Quantitative Proteome Analysis of Glycoproteins
Patent # 7183118B2 | 2003

Affinity Capture Of Peptides By Microarray And Related Methods
Patent # 7,794,947 | 2003

Contact for Research Inquiries

400 N. Broadway
Smith Building, Room 4011
Baltimore, MD 21231 map
Phone: 410-502-8149

Academic Affiliations & Courses

Graduate Program Affiliation

Graduate Faculty of Pathobiology Graduate Training Program

Courses and Syllabi

  • Techniques in Glycobiology (ME340.710)
    Johns Hopkins University School of Medicine
    2013 - 2017
  • Fundamentals of Glycobiology (ME340.709)
    Johns Hopkins University School of Medicine
    2012 - 2017
  • The Role of Chromatography and Mass Spectrometry in Biological Research (ME330.804)
    Johns Hopkins University School of Medicine
    2007 - 2012

Activities & Honors

Honors

  • Pre-doctoral Fellowship, American Heart Association, 1997
  • Technology Development Award, Cell Signaling Technology, 2003
  • Young Scientist Award, Human Proteome Organization (HUPO), 2004
  • Selected to Board of Director, United States Human Proteomic Organization, 2012
  • Elected as a Council Member, World Human Proteomic Organization (HUPO), 2015

Memberships

  • American Chemical Society (ACS)
  • American Society for Biochemistry and Molecular Biology (ASBMB)
  • American Society for Mass Spectrometry (ASMS)
  • Society for Glycobiology
  • United States Human Proteomic Organization (US HUPO)
  • Chinese American Society for Mass Spectrometry (CASMS), 2015
  • American Association for Cancer Research (AACR), 2015
  • World Human Proteomic Organization (HUPO), 2010

Professional Activities

  • Member, Editorial Board of Clinical Proteomics, 2011
  • Associate Editor, Journal of Intergrated-omics, 2010
  • Guest editor of two special issues, Clinical Proteomics on Glycoproteomics, 2008
  • Associate Editor, Clinical Proteomics, 2011
  • Guest Editor, Special Issue of Clinical Proteomics on Glycoproteomics and Glycomics, 2013
  • Member, Editorial Board of Journal of Bioinformatics, 2014
  • Member, Search Committee on Endowed Chairs of Bloomberg Distinguished Professorships, 2015
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