We are applying morphological, biochemical, and genetic approaches to the problem of preinvasive cancer and what determines its progression to invasive disease. Our main focus has been on breast cancer and thyroid cancer, and on the role of specific genes, such as telomerase, or the Vitamin D receptor, in the progression to invasive cancer. We are currently using genome-wide cDNA and methylation arrays as well as next-generation sequencing technologies to identify changes in gene expression, DNA methylation, and DNA copy number variation during tumor progression, and following up on promising leads by developing DNA methylation markers, real-time RT-PCR assays, and immunohistochemical analyses for individual genes and molecular signatures with prognostic and predictive significance in the clinical setting.
Technology Expertise Keywords
Creator and Curator of integrated clinical-pathological research databases.
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McKelvey BA, Zeiger MA, Umbricht CB. Characterization of TERT and BRAF copy number variation in papillary thyroid carcinoma: An analysis of the cancer genome atlas study. Genes Chromosomes Cancer 2021; 60:403-409; doi: 10.1002/gcc.22928
McKelvey BA, Gilpatrick T, Wang Y, Timp W, Umbricht CB (corresponding), Zeiger MA. Characterization of Allele-Specific Regulation of Telomerase Reverse Transcriptase in Promoter Mutant Thyroid Cancer Cell Lines. [published online ahead of print, 2020 May 4]. Thyroid. 2020; 30:1470–1481. PMID: 32228178; doi:10.1089/thy.2020.0055
Fackler MJ, Cho S, Cope LM, Gabrielson E, Visvanathan K, Wilsbach K, Meir-Levi D, Lynch CF, Marks J, Geradts J, Regan MM, Viale G, Wolff AC, Sukumar S, Umbricht CB. DNA Methylation Markers Predict Recurrence-Free Interval in Triple-Negative Breast Cancer. NPJ Breast Cancer. 2020;6:1-6. doi: 10.1038/s41523-020-0145-3.
Cho S, Zeiger MA, Umbricht CB (corresponding), Cope LM. Measuring DNA copy number variation using high-density methylation microarrays. J Comp Biol. 2019; 26:295-304. PMCID:6786966.
Liu Z, Sahli Z, Wang Y, Wolff AC, Cope LM, Umbricht CB. Young age at diagnosis is associated with worse prognosis in the Luminal A breast cancer subtype: a retrospective institutional cohort study. Breast Cancer Res Treat. 2018;172:689-702. doi: 10.1007/s10549-018-4950-4. PubMed PMID: 30225619
Cloning & Expression of the 14 kD subunit of human replication protein A
Patent # 179837 |