July 2014

Pharmacology and Molecular Sciences Archives

1. Calcaterra NE, Barrow JC. Classics in Chemical Neuroscience: Diazepam (Valium). ACS Chem. Neurosci. 2014 Apr 16;5(4):253-60.
Key words: Diazepam, Valium, benzodiazepine, GABA, anxiety, hypnotic, sedative

1. Hersman EM, Bumpus NN. A targeted proteomics approach for profiling murine cytochrome P450 expression. J Pharmacol Exp Ther. 2014 May;349(2):221-8.
Key words: cytochrome P450; mass spectrometry; drug metabolism

1. Webster MR, Kamat C, Connis N, Zhao M, Weeraratna AT, Rudek MA, Hann CL, Freel Meyers CL. Bisphosphonamidate clodronate prodrug exhibits selective cytototxic activity against melanoma cell lines. Mol Cancer Ther. 2014 Feb;13(2):297-306.
Key words: prodrug, bisphosphonate, melanoma, clodronate

1. Kiwamoto T, Brummet ME, Wu F, Motari MG, Smith DF, Schnaar RL, Zhu Z, Bochner BS. Mice deficient in the St3gal3 gene product ?2,3 sialyltransferase (ST3Gal-III) exhibit enhanced allergic eosinophilic airway inflammation. J Allergy Clin Immunol. 2014 Jan;133(1):240-247.
Key words: asthma, airway, inflammation, eosinophil, lung

1. Avery LB and Bumpus NN. Valproic acid is a novel activator of AMP-activated protein kinase and decreases liver mass, hepatic fat accumulation, and serum glucose in obese mice. Molecular Pharmacology. 2014 Jan;85(1):1-10.
Key words: Valproic Acid; AMP-activated Protein Kinase; Metabolomics; Mass Spectrometry; Cytochrome P450

2. Teng X, Dayhoff-Brannigan M, Cheng WC, Gilbert CE, Sing CN, Diny NL, Wheelan SJ, Dunham MJ, Boeke JD, Pineda FJ, Hardwick JM. Genome-wide consequences of deleting any single gene. Mol Cell. 2013 Nov 21;52(4):485-94.
Key words: genome evolution, yeast, cancer, amino acid-sensing, cell death

1. Byrum SD, Taverna SD, and Tackett AJ. (2013) Purification of a specific native genomic locus for proteomic analysis. Nucleic Acids Res. 2013 Nov 1;41(20), e195, doi:10.1093/nar/gkt822
Key words: Epigenetics, Transcription, Histone post-translational modifications, Acetylation, Mass spectromery

2. Hu S, Wan J, Su Y, Song Q, Zeng Y, Nguyen HN, Shin J, Cox E, Rho H-S, Woodard C, Xia S, Liu S, Lu H, Ming G, Wade H, Song H, Qian J, Zhu H. DNA methylation presents distinct binding sites for human transcription factors. eLife. 2013; 2:e00726.
Key words: DNA methylation, transcription, protein microarrays, KLF4, CpG

3. Lade JM, Avery LB, Bumpus NN. 2013. Human biotransformation of the non-nucleoside reverse transcriptase inhibitor rilpivirine and a cross species metabolism comparison. Antimicrobial Agents and Chemotherapy. 2013 Oct; 57(10):5067-5079.
Key words: cytochrome P450; HIV; antiretroviral; mass spectrometry; drug metabolism

4. To EE, Hendrix CW, Bumpus NN. 2013. Dissimilarities in the metabolism of antiretroviral drugs used in HIV pre-exposure prophylaxis in colon and vagina tissues. Biochemical Pharmacology. 2013 Oct 1; 86(7):979-990.
Key words: cytochrome P450; HIV; antiretroviral; mass spectrometry; drug metabolism

1. Hu S, Feng Y, Henson B, Wang B, Huang X, Li M, Desai P, Zhu H. VirD - A virion display array for profiling functional membrane proteins. Anal Chem. 2013 Sep 3;85(17):8046?8054.
Key words: VirD; multi-pass membrane proteins, virions, protein microarrays, GPCR

2. Woodard CL, Goodwin CR, Wan J, Xia S, Hu J, Newman RH, Zhang J, Hayward SD, Qian J, Laterra J, Zhu H. Profiling the dynamics of a human phosphorylome reveals new components in HGF/c-Met signaling. PLoS One 2013 Sept 2; 8(9):e72671.
Key words: Phosphorylation, lysate, protein microarrays, c-Met, signal transduction

1. Morris F, Vierling R, Boucher L, Bosch J, Freel Meyers C.L. DXP synthase-catalyzed C-N bond formation: nitroso substrate specificity studies guide selective inhibitor design. ChemBioChem. 2013 Jul 22;14(11):1309-1315.
Key words: isoprenoid biosynthesis, selective enzyme inhibitors, thiamin diphosphate


1. Smith J, Vierling RJ, Freel Meyers CL. Selective inhibition of E. coli 1-deoxy-D-xylulose-5-phosphate synthase by acetylphosphonates. MedChemComm. 2012; 3(1): 65-67.
Key words: isoprenoid biosynthesis, DXP synthase, anti-infectives, acetylphosphonate

1. Patel H, Nemeria N, Brammer L, Freel Meyers C, Jordan F. Observation of thiamin-bound intermediates and microscopic rate constants for their interconversion on 1-deoxy-D-xylulose 5-phosphate synthase: 600-fold rate acceleration of pyruvate decarboxylation by D-glyceraldehyde-3-phosphate. J Am Chem Soc 2012;134:18374-79.
Key words: thiamin diphosphate, isoprenoid biosynthesis, anti-infectives

1. Mountney A, Zahner MR, Sturgill ER, Riley CJ, Aston JW, Oudega M, Schramm LP, Hurtado A, Schnaar RL. Sialidase, chondroitinase ABC and combination therapy after spinal cord contusion injury. J Neurotrauma. 2012 Aug 31. [Epub ahead of print]
Key words: Spinal cord injury; axon regeneration; ganglioside; motor behavior; serotonergic axons

2. Shim JS, Rao R, Beebe K, Neckers L, Han I, Nahta R, Liu JO. Selective inhibition of HER2-positive breast cancer cells by the HIV protease inhibitor nelfinavir. J Natl Cancer Inst. 2012 Oct 5. [Epub ahead of print]
Key words include: Nelfinavir, Johns Hopkins Drug Library, Her2, breast cancer, and drug resistance

3. Sturgill ER, Aoki K, Lopez PH, Colacurcio D, Vajn K, Lorenzini I, Majic S, Yang WH, Heffer M, Tiemeyer M, Marth JD, Schnaar RL. Biosynthesis of the major brain gangliosides GD1a and GT1b. Glycobiology. 2012 Oct;22(10):1289-301. Epub 2012 Jun 26.
Key words: Brain, ganglioside, myelin, sialic acid, sialyltransferase

1. Byrum SD, Raman A, Taverna SD, Tackett AJ. ChAP-MS: A method for identification of proteins and histone posttranslational modifications at a single genomic locus. Cell Reports. 2012 July 26;2(1):198-205.
Key words: Histone, chromatin, affinity purification, transcription, mass spectrometry

2. Jung KO, Khan AM, Tan BY, Hu Y, Simon GG, Nascimento EJ, Lemonnier F, Brusic V, Miotto O, Tan TW, Marques ET, Dhalia R, Salmon J, August JT. West nile virus T-cell ligand sequences shared with other flaviviruses: a multitude of variant sequences as potential altered Peptide ligands. J Virol. 2012 July; 86(14):7616-7624.
Key words: West Nile virus, T-cell ligand, flaviviruses, altered peptide ligands, immune cross-reactivity

1. Barrow, JC. Inhibitors of catechol-o-methyltransferase. CNS Neurol Disord Drug Targets, 2012 May;11(3):324-332.
Key words: Catechol-O-methyltransferase; COMT; dopamine; L-DOPA; Parkinson's disease

2. Calabrese V, Cornelius C, Dinkova-Kostova AT, Iavicoli I, Di Paola R, Koverech A, Cuzzocrea S, Rizzarelli E, Calabrese EJ. Cellular stress responses, hormetic phytochemicals and vitagenes in aging and longevity. Biochim Biophys Acta. 2012 May;1822(5):753-83.
Key words: Nrf2, Keap1, stress, sulforaphane, hormesis

3. Dedecker P, Mo GCH, Dertinger T, Zhang J. A widely accessible method for superresolution fluorescence imaging of living systems. Proc. Natl. Acad. Sci. USA. 2012 Jul 3;109(27):10909-10914. [Epub ahead of print]
Key words: subdiffraction-limit, two-color imaging, membrane rafts

4. Dinkova-Kostova AT, Kostov R. Glucosinolates and isothiocyanates in health and disease. Trends Mol Med. 2012 Jun;18(6):337-47.
Key words: NQO1, sulforaphane, phenethylisothiocyanate, cancer, neurodegenerative disease

5. Ibbotson SH, Dawe RS, Dinkova-Kostova AT, Weidlich S, Farr PM, Ferguson J, Wolf CR, Smith G. Glutathione S-transferase genotype is associated with sensitivity to psoralen-UVA (PUVA) photochemotherapy. Br J Dermatol. 2012; 166: 380-388.
Key words: GST, psoralen, UVA, 8-MOP, photochemotherapy

6. Kalra S, Knatko EV, Zhang Y, Honda T, Yamamoto M, Dinkova-Kostova AT. Highly potent activation of Nrf2 by topical tricyclic bis(cyano enone): Implications for protection against UV radiation during thiopurine therapy. Cancer Prev Res (Phila). 2012 Jul;5(7):973-81.
Key words: cyanoenone, Nrf2, NQO1, skin cancer, ultraviolet radiation

7. Zheng S, Laxmi Y R S, David E, Dinkova-Kostova AT, Shiavoni KH, Ren Y, Zheng Y, Trevino I, Bumeister R, Ojima I, Wigley WC, Bliska J, Mierke DF, Honda T. Synthesis, chemical reactivity as Michael acceptors, and biological potency of monocyclic cyanoenones, novel and highly potent anti-inflammatory and cytoprotective agents. J Med Chem. 2012 May 24; 55(10): 4837-4846.
Key words: Keap1, Michael acceptor, NQO1, iNOS, cyanoenone

1. Fahey JW, Wehage SL, Holtzclaw WD, Kensler TW, Egner PA, Shapiro TA, Talalay P. Protection of humans by plant glucosinolates: efficiency of conversion of glucosinolates to isothiocyanates by the gastrointestinal microflora. Cancer Prev Res (Phila). 2012 Mar 13. [Epub ahead of print].
Key words: isothiocyanates, gastrointestinal microflora, glucosinolates, chemoprotection, crucifers

2. Ofengeim D, Chen YB, Miyawaki T, Li H, Sacchetti S, Flannery RJ, Alavian KN, Pontarelli F, Roelofs BA, Hickman JA, Hardwick JM, Zukin RS, Jonas EA. N-terminally cleaved Bcl-xL mediates ischemia-induced neuronal death. Nat Neurosci. 2012 Feb 26. [Epub ahead of print].
Key words: Stroke, Apoptosis, Neuron, ABT-737, Knockin mice

3. Sample V, Dipilato LM, Yang JH, Ni Q, Saucerman JJ, Zhang J. Regulation of nuclear PKA revealed by spatiotemporal manipulation of cyclic AMP. Nat Chem Biol 2012 Feb 26. [Epub ahead of print]
Key words: cAMP; spatiotemporal control; fluorescence imaging; mathematic modeling; functional nuclear PKA

1. Hostetler ED, Sanabria-Bohórquez S, Fan H, Zeng Z, Gammage L, Miller P, O'Malley S, Connolly B, Mulhearn J, Harrison ST, Wolkenberg SE, Barrow JC, Williams DL Jr, Hargreaves RJ, Sur C, Cook JJ. [18F]Fluoroazabenzoxazoles as potential amyloid plaque PET tracers: synthesis and in vivo evaluation in rhesus monkey. Nuclear Medicine and Biology. 2011; 38: 1193-1203.
Key words: Alzheimer's disease; Amyloid plaque, PET; Monkey; Fluorine-18; White matter

1. Li R, Zhu J, Xie Z, Liao G, Liu J, Chen MR, Hu S, Woodard C, Lin J, Taverna SD, Desai P, Ambinder RF, Hayward GS, Qian J, Zhu H, Hayward SD. Conserved herpesvirus kinases target the DNA damage response pathway and TIP60 histone acetyltransferase to promote virus replication. Cell Host Microbe 2011; 10(4):390-400.
Key words: pathogen-host interaction, viral kinase, TIP60, herpesvirus, virus replication

1. Alavian KN, Li H, Collis L, Bonanni L, Zeng L, Sacchetti S, Lazrove E, Nabili P, Flaherty B, Graham M, Chen Y, Messerli SM, Mariggio MA, Rahner C, McNay E, Shore GC, Smith PJ, Hardwick JM, Jonas EA. Bcl-x(L) regulates metabolic efficiency of neurons through interaction with the mitochondrial F(1)F(O) ATP synthase. Nat Cell Biol. 2011 Sep 18; 13(10):1224-1233.
Key words: Bcl-xL, mitochondria, energetics, neurons, ATP synthase

2. Brammer LA, Smith JM, Wade H, Freel Meyers C. 1-Deoxy-D-Xylulose 5-phosphate synthase: A novel random sequential mechanism in thiamine Diphosphate-dependent enzymology. J Biol Chem. 2011 Oct 21; 286(42):36522-31.
Key words: mthylerythritol phosphate pathway, isoprenoid biosynthesis, deoxy-D-xylulose 5-phosphate synthase

3. Chen YB, Aon MA, Hsu YT, Soane L, Teng X, McCaffery JM, Cheng WC, Qi B, Li H, Alavian KN, Dayhoff-Brannigan M, Zou S, Pineda FJ, O'Rourke B, Ko YH, Pedersen PL, Kaczmarek LK, Jonas EA, Hardwick JM. Bcl-xL regulates mitochondrial energetics by stabilizing the inner membrane potential. J Cell Biol. 2011 Oct 17;195(2):263-76.
Key words: Bcl-xL, mitochondria, neurons, apoptosis, ATP synthase

4. Webster MR, Zhao M, Rudek MA, Hann CL, Freel Meyers CL. Bisphosphonamidate clodronate prodrug exhibits potent anticancer activity in non-small-cell lung cancer cells. J Med Chem. 2011 Oct 13;54(19):6647-56.
Key words: bisphosphonate, bisphosphonamidate prodrug, clodronate

1. Healy ZR, Liu H, Holtzclaw WD, Talalay P. Inactivation of tautomerase activity of macrophage migration inhibitory factor by sulforaphane: A potential biomarker for anti-inflammatory intervention. Cancer Epidemiol Biomarkers Prev. 2011 July;20(7): 1516-1523.
Key words: Macrophage migration inhibitory factor (MIF), Sulforaphane, Biomarker, Anti-inflammatory activity

2. Lu J-Y, Lin Y-Y, Sheu J-C, Wu J-T, Lee F-J, Chen Y, Lin M-I, Chiang F-T, Tai T-Y, Berger SL, Zhao Y, Tsai K-S, Zhu H, Chuang L-M, Boeke JD. Acetylation of AMPK controls intrinsic aging independently of caloric restriction. Cell. 2011 Sept; 146(6): 969-979.
Key words: Intrinsic yeast ageing, Sip2 acetylation, Snf1 inhibition, Sch9, and NuA4 complex

Return to top of Publications - Pharmacology Archives

Search Publications
July 2014 articles:
New CIRB Combined Informed Consent/Authorization Templates and New Version of King Khaled Eye Specialist Hospital Informed Consent Template
Visit Animal Care and Use Committee Website
Current Classes
Funding from Foundations/Private Sources
Marilyn Hilton Award for Innovation in MS Research
Google's Advanced Technology and Projects (ATAP) - Project Ara
W.W. Smith Charitable Trust - Heart Research Grant Program for 2014-2015
Burroughs Wellcome Fund - Career Award for Medical Scientists
"Interinstitutional Assurance" Agreement (between Johns Hopkins and a company receiving an NIH research award)

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