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David R.M. Graham, M.S., Ph.D.

Executive Director, Center for Resources in Integrative Biology
Associate Professor of Molecular and Comparative Pathobiology
Male

Titles

  • Executive Director, Center for Resources in Integrative Biology
  • Associate Professor of Molecular and Comparative Pathobiology
  • Associate Professor of Medicine

Centers & Institutes

  • Basic Biomedical Sciences, Institute for
  • Resources in Integrative Biology, Center for

Research Interests

H/SIV pathogenesis, and neuropathogenesis; Cardiovascular disease; High technology development

Biography

Dr. David R.M. Graham is an assistant professor of molecular and comparative biology at the Johns Hopkins University School of Medicine. He holds a secondary appointment in the Division of Cardiology. Additionally, he serves as the School of Medicine Executive Director for the Center for Resources in Integrative Biology, a School of Medicine and School of Public Health joint faculty initiative.

His research focuses on understanding the consequences of HIV interactions with the immune system, the resulting pathogenesis and how to sabotage these interactions.

Dr. Graham completed his undergraduate studies in biomedical and health sciences at the University of Guelph and his master’s work in biology at McMaster University, both in Ontario, Canada. After a two-year break from academics, he relocated to Baltimore and returned to graduate school at Johns Hopkins, where he obtained his Ph.D. in biochemistry, cellular and molecular biology. In 2004, he joined the faculty of Johns Hopkins as a research associate in the Division of Cardiology, and in 2009 he became an assistant professor.

Dr. Graham is a member of several professional societies and serves on the editorial boards of PROTEOMICS and Frontiers Immunology. He has published more than two dozen peer reviewed articles and one book chapter, and has presented his work at several national and international conferences. . He has served as co-investigator or co-principal investigator on several research grants funded by the National Institutes of Health, and holds one patent.

Languages

  • English

Memberships

American Association for the Advancement of Science

American Society for Microbiologists

American Heart Association

Human Proteome Organization

Additional Resources +
  • Education +

    Education

    • Ph.D., The Johns Hopkins University School of Medicine, Baltimore, MD, 2004, Biochemistry, Cellular and Molecular Biology
    • M.Sc., McMaster University, Hamilton, Ontario, Canada, 1990, Biology
    • B.Sc., University of Guelph, Guelph, Ontario, Canada, 1990, Biomedical and Health Sciences

    Fellowships

    • The Johns Hopkins University School of Medicine, Baltimore, MD, 2006, Senior Research Fellow, Cardiology/Proteomics
  • Research & Publications +

    Research Summary

    Dr. Graham merges his research in cardiology and virology to study HIV-induced cardiomyopathy, an important emerging area in cardiovascular medicine.

    His research in virology is concentrated on understanding the differences between viruses with divergent pathologies, including the ability to differentially induce apoptosis by a IFN a/B induced expression of TRAIL, in collaboration with Gene Shearer (NIH) and Jean-Phillipe Herbeuval (Necker Institute, France), and viruses with the ability to induce neurotoxicity in collaboration with Drs. Zink and Clements (retrovirus laboratory).

    In cardiology, Dr. Graham focuses on the molecular phenotyping of transgenic rabbits over expressing alpha-myosin heavy chain, which has been shown to be cardioprotective against pacing induced heart failure. In this complex experimental model, Dr. Graham has been able to elucidate changes in the proteome caused by the transgene alone as compared to the background strain and differences between all groups under pacing induced heart failure. He has been collaborating on an investigation of the role of lipid rafts in the control of SA nodal cell function, and has developed ultra-sensitive mass-spectrometry methods to compare compositional changes in the lipid raft proteome between control and stimulated SA nodal cells.

    Selected Publications

    1. Brown, J.M., N.J. Shaw, and D.R. Graham. “The first five years: a mixed methods study investigating reflections on working as a hospital consultant.” JRSM Short Rep, 2013. 4(5): p. 2042533313476686.
    2. Linde, M.E., et al. “The conserved set of host proteins incorporated into HIV-1 virions suggests a common egress pathway in multiple cell types.” J Proteome Res, 2013. 12(5): p. 2045-54.
    3. Nzowa, L.K., et al. “Two new tryptophan derivatives from the seed kernels of Entada rheedei: effects on cell viability and HIV infectivity.” Fitoterapia, 2013. 87: p. 37-42.
    4. Tavano, B., et al. “Ig-like transcript 7, but not bone marrow stromal cell antigen 2 (also known as HM1.24, tetherin, or CD317), modulates plasmacytoid dendritic cell function in primary human blood leukocytes.” J Immunol, 2013. 190(6): p. 2622-30.
    5. Tovar, Y.R.L.B., et al. “Adenosine Triphosphate Released from HIV-Infected Macrophages Regulates Glutamatergic Tone and Dendritic Spine Density on Neurons.” J Neuroimmune Pharmacol, 2013.

    Lab

    I have been directly applying mass spectrometry and bioinformatics toward gaining understanding of the mechanisms involved in HIV pathogenesis in both the periphery and CNS for over a decade. It is becoming very clear that there is an integrated response to HIV infection, and that metabolism works hand in hand with both the development and response to disease. From the moment that innate immune responses begin to HIV metabolic responses are engaged, from increased demand from NADPH in response to oxidative stress, to responses kynurenine metabolites that can alter both immune function in the periphery and in the CNS work co-operatively exitotoxic mechanisms mediated by excess extracellular glutamate. In this application, we are extremely well-positioned to study the cross talk between metabolism and neuroinflammation under cART therapy, and how therapeutic approaches targeting glutamate excitotoxicity might be able to prevent both the ongoing CNS damage under cART and potentially protect against treatments intended to purge latent viral reservoirs.

    In additional to very targeted hypothesis-driven studies, my group has been taking advantage of unbiased approaches to understanding disease by integrating information from many experimental sources. Our group has extensive experience in proteomics and more recently, we have extended our experience to the area of metabolomics and lipidomics. In my role as the director for the School of Medicine for the Center for Resources in Integrative Biology (CRIB), we have been combining multiple data sources with MPP Pro software after careful validation through our method specific workflows. We have used these approaches to reveal new potential biomarkers of HAND as described within this application. In this application, I will work directly with Dr. Slusher as the co-PI of project 3, bringing experience in bioinformatics and data integration to help elucidate how antagonists of AMPA receptor and other glutaminergic pathways mediate their effects to prevent HAND in the TAT mouse model. Also as the co-PI for the BSL-3/Analytical Measures core, I will not only support similar measures in other projects, but will support novel methods like phosphopeptide enrichment in project 2 and metabolite and protein measures from the CSF and brain in project 1.

  • Academic Affiliations & Courses +

    Graduate Program Affiliation

    Cellular and Molecular Medicine

  • Activities & Honors +

    Honors

    Travel Fellowship Award, 11th Conference on Retroviruses and Opportunistic Infections, 2004

    Travel Fellowship Award, 10th Conference on Retroviruses and Opportunistic Infections, 2003

    President’s Stock Option Award, Science Applications International Corporation, 1999 and 2000

    Professional Activities

    Editorial board member, PROTEOMICS

    Editorial board member, Frontiers Immunology

  • Videos & Media +
  • Events +
  • Contact & Locations +

    Department/Division

    • Molecular and Comparative Pathobiology
    • Medicine - Cardiovascular

    For Research Inquiries Contact

    733 N. Broadway, MRB 835
    Baltimore, MD 21205

    Retrovirus Laboratory: 410-955-9770
    Fax: 410-955-9823
    dgraham@jhmi.edu

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