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Charles George Eberhart, MD PhD

Professor of Pathology
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The Johns Hopkins Hospital

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Call 410-464-6641 (8a.m. to 6p.m., EST, Mon-Fri)

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  • Professor of Pathology
  • Professor of Oncology
  • Associate Professor of Ophthalmology

Centers & Institutes

  • Sidney Kimmel Comprehensive Cancer Center




  • English
  • German
Additional Resources +
  • Education +


    • University of Texas Southwestern Medical School ( Dallas TX) (1997)


    • Johns Hopkins University School of Medicine / Pathology ( Baltimore MD) (2001)


    • American Board of Pathology / Anatomic Pathology (2003)
    • American Board of Pathology / Neuropathology (2003)
  • Research & Publications +

    Selected Publications

    Research Summary

    The Eberhart laboratory studies the molecular genetics of medulloblastoma, glioblastoma, uveal melanoma and other tumors of the brain and eye. The focus is on the Notch and Hedgehog pathways, which play a key role in both normal brain and eye development and in neoplasia. We are currently analyzing the effects of Notch and Hedgehog pathway blockade on cancer stem cells in brain and eye tumors. We are also investigating the basis of the sometimes antagonistic effects of various Notch receptors in medulloblastoma and how this relates to their roles in neural stem-cell proliferation and differentiation. Finally, we are examining the role of c-Myc in the stem cell phenotype of cancer.

    Journal Citations

    Chu, L.C.; Eberhart, C.G.; Grossman, S.A.; Herman, J.G. Epigenetic silencing of multiple genes in primary CNS lymphoma. Int J Cancer. 2006 Nov 15;119(10):2487-2491.

    Dang, L.; Fan, X.; Chaudhry, A.; Wang, M.; Gaiano, N.; Eberhart, C.G. Notch3 signaling initiates choroid plexus tumor formation. Oncogene. 2006 Jan 19;25(3):487-491.

    Dixit, V.D.; Weeraratna, A.T.; Yang, H.; Bertak, D.; Cooper-Jenkins, A.; Riggins, G.J.; Eberhart, C.G.; Taub, D.D. Ghrelin and the growth hormone secretagogue receptor constitute a novel autocrine pathway in astrocytoma motility. The Journal of biological chemistry. 2006 Jun 16;281(24):16681-16690.

    Eberhart, C.G.; Copeland, J.; Abel, T.W. Brief Report: S6 Ribosomal Protein Phosphorylation in Autistic Frontal Cortex and Cerebellum: a Tissue Array Analysis. J Autism Dev Disord. 2006 Jul 15;36(1131-1135).

    Fan, X.; Matsui, W.; Khaki, L.; Stearns, D.; Chun, J.; Li, Y.M.; Eberhart, C.G. Notch pathway inhibition depletes stem-like cells and blocks engraftment in embryonal brain tumors. Cancer Res. 2006 Aug 1;66(15):7445-7452.

    Gallia, G.L.; Rand, V.; Siu, I.M.; Eberhart, C.G.; James, C.D.; Marie, S.K.; Oba-Shinjo, S.M.; Carlotti, C.G.; Caballero, O.L.; Simpson, A.J.; Brock, M.V.; Massion, P.P.; Carson, B.S., Sr.; Riggins, G.J. PIK3CA gene mutations in pediatric and adult glioblastoma multiforme. Mol Cancer Res. 2006 Oct;4(10):709-714.

    Park, J.T.; Li, M.; Nakayama, K.; Mao, T.L.; Davidson, B.; Zhang, Z.; Kurman, R.J.; Eberhart, C.G.; Shih Ie, M.; Wang, T.L. Notch3 gene amplification in ovarian cancer. Cancer Res. 2006 Jun 15;66(12):6312-6318.

    Stearns, D.; Chaudhry, A.; Abel, T.W.; Burger, P.C.; Dang, C.V.; Eberhart, C.G. c-myc overexpression causes anaplasia in medulloblastoma. Cancer Res. 2006 Jan 15;66(2):673-681.

    Bar, E.E.; Chaudhry, A.; Farah, M.H.; Eberhart, C.G. Hedgehog signaling promotes medulloblastoma survival via Bc/II. Am J Pathol. 2007 Jan;170(1):347-355.

    Bar, E.E.; Chaudhry, A.; Lin, A.; Fan, X.; Schreck, K.; Matsui, W.; Piccirillo, S.; Vescovi, A.L.; DiMeco, F.; Olivi, A.; Eberhart, C.G. Cyclopamine-mediated hedgehog pathway inhibition depletes stem-like cancer cells in glioblastoma. Stem Cells. 2007 Oct;25(10):2524-2533.

    Eberhart, C.G. In search of the medulloblast: neural stem cells and embryonal brain tumors. Neurosurg Clin N Am. 2007 Jan;18(1):59-69, viii-ix.

    Li, T.; Wen, H.; Brayton, C.; Das, P.; Smithson, L.A.; Fauq, A.; Fan, X.; Crain, B.J.; Price, D.L.; Golde, T.E.; Eberhart, C.G.; Wong, P.C. Epidermal growth factor receptor and notch pathways participate in the tumor suppressor function of gamma-secretase. J Biol Chem. 2007 Nov 2;282(44):32264-32273.

    Pardo, C.A.; Eberhart, C.G. The neurobiology of autism. Brain Pathol. 2007 Oct;17(4):434-447.

    Rodriguez, F.J.; Eberhart, C.; O'Neill, B.P.; Slezak, J.; Burger, P.C.; Goldthwaite, P.; Wu, W.; Giannini, C. Histopathologic grading of adult medulloblastomas. Cancer. 2007 Jun 15;109(12):2557-2565.

    Bar, E.E.; Lin, A.; Tihan, T.; Burger, P.C.; Eberhart, C.G. Frequent gains at chromosome 7q34 involving BRAF in pilocytic astrocytoma. J Neuropathol Exp Neurol. 2008 Sep;67(9):878-887.

    Briggs, K.J.; Corcoran-Schwartz, I.M.; Zhang, W.; Harcke, T.; Devereux, W.L.; Baylin, S.B.; Eberhart, C.G.; Watkins, D.N. Cooperation between the Hic1 and Ptch1 tumor suppressors in medulloblastoma. Genes Dev. 2008 Mar 15;22(6):770-785.

    Briggs, K.J.; Eberhart, C.G.; Watkins, D.N. Just say no to ATOH: how HIC1 methylation might predispose medulloblastoma to lineage addiction. Cancer Res. 2008 Nov 1;68(21):8654-8656.

    Chen, C.S.; Miller, N.R.; Lane, A.; Eberhart, C. Third cranial nerve palsy caused by intracranial extension of a sino-orbital natural killer T-cell lymphoma. J Neuroophthalmol. 2008 Mar;28(1):31-35.

    Eberhart, C.G. Even cancers want commitment: lineage identity and medulloblastoma formation. Cancer Cell. 2008 Aug 12;14(2):105-107.

    Fan, X.; Eberhart, C.G. Medulloblastoma stem cells. J Clin Oncol. 2008 Jun 10;26(17):2821-2827.

    Gessi, M.; Giangaspero, F.; Lauriola, L.; Gardiman, M.; Scheithauer, B.W.; Halliday, W.; Hawkins, C.; Rosenblum, M.K.; Burger, P.C.; Eberhart, C.G. Embryonal Tumors With Abundant Neuropil and True Rosettes: A Distinctive CNS Primitive Neuroectodermal Tumor. Am J Surg Pathol. 2008 Nov 3.

    Li , Y.; Guessous, F.; Johnson, E.B.; Eberhart, C.G.; Li, X.N.; Shu, Q.; Fan, S.; Laterra, J.; Schiff, D.; Abounader, R. Functional and molecular interactions between the HGF/c-Met pathway and c-Myc in large cell medulloblastoma. Laboratory Investigation. 2008 Feb;88:98-111.

    Macgregor, A.M.; Eberhart, C.G.; Fraig, M.; Lu, J.; Halushka, M.K. Tissue Inhibitor of Matrix Metalloproteinase-3 Levels in the Extracellular Matrix of Lung, Kidney, and Eye Increase With Age. J Histochem Cytochem. 2008 Oct 27.

    Siu, I.M.; Bai, R.; Gallia, G.L.; Edwards, J.B.; Tyler, B.M.; Eberhart, C.G.; Riggins, G.J. Coexpression of neuronatin splice forms promotes medulloblastoma growth. Neuro Oncol. 2008 Oct;10(5):716-724.

    Yi, J.M.; Tsai, H.C.; Glockner, S.C.; Lin, S.; Ohm, J.E.; Easwaran, H.; James, C.D.; Costello, J.F.; Riggins, G.; Eberhart, C.G.; Laterra, J.; Vescovi, A.L.; Ahuja, N.; Herman, J.G.; Schuebel, K.E.; Baylin, S.B. Abnormal DNA methylation of CD133 in colorectal and glioblastoma tumors. Cancer Res. 2008 Oct 1;68(19):8094-8103.

    Ayres, B.; Miller, N.R.; Eberhart, C.G.; Dibernardo, C.W. Ultrasound features of orbital granular cell tumor. Ophthal Plast Reconstr Surg. 2009 Jul-Aug;25(4):320-322.

    Barnes, M.; Eberhart, C.G.; Collins, R.; Tihan, T. Expression of p75NTR in fetal brain and medulloblastomas: evidence of a precursor cell marker and its persistence in neoplasia. J Neurooncol. 2009 Apr;92(2):193-201.

    Gessi, M.; Giangaspero, F.; Lauriola, L.; Gardiman, M.; Scheithauer, B.W.; Halliday, W.; Hawkins, C.; Rosenblum, M.K.; Burger, P.C.; Eberhart, C.G. Embryonal tumors with abundant neuropil and true rosettes: a distinctive CNS primitive neuroectodermal tumor. Am J Surg Pathol. 2009 Feb;33(2):211-217.

    Macgregor, A.M.; Eberhart, C.G.; Fraig, M.; Lu, J.; Halushka, M.K. Tissue inhibitor of matrix metalloproteinase-3 levels in the extracellular matrix of lung, kidney, and eye increase with age. J Histochem Cytochem. 2009 Mar;57(3):207-213.

    Mullendore, M.E.; Koorstra, J.B.; Li, Y.M.; Offerhaus, G.J.; Fan, X.; Henderson, C.M.; Matsui, W.; Eberhart, C.G.; Maitra, A.; Feldmann, G. Ligand-dependent Notch signaling is involved in tumor initiation and tumor maintenance in pancreatic cancer. Clin Cancer Res. 2009 Apr 1;15(7):2291-2301.

    Nasonkin, I.; Mahairaki, V.; Xu, L.; Hatfield, G.; Cummings, B.J.; Eberhart, C.; Ryugo, D.K.; Maric, D.; Bar, E.; Koliatsos, V.E. Long-term, stable differentiation of human embryonic stem cell-derived neural precursors grafted into the adult mammalian neostriatum. Stem Cells. 2009 Oct;27(10):2414-2426.

    Northcott, P.A.; Nakahara, Y.; Wu, X.; Feuk, L.; Ellison, D.W.; Croul, S.; Mack, S.; Kongkham, P.N.; Peacock, J.; Dubuc, A.; Ra, Y.S.; Zilberberg, K.; McLeod, J.; Scherer, S.W.; Sunil Rao, J.; Eberhart, C.G.; Grajkowska, W.; Gillespie, Y.; Lach, B.; Grundy, R.; Pollack, I.F.; Hamilton, R.L.; Van Meter, T.; Carlotti, C.G.; Boop, F.; Bigner, D.; Gilbertson, R.J.; Rutka, J.T.; Taylor, M.D. Multiple recurrent genetic events converge on control of histone lysine methylation in medulloblastoma. Nat Genet. 2009 Apr;41(4):465-472.

    Oster, S.F.; Ebrahimi, K.B.; Eberhart, C.G.; Schein, O.D.; Stark, W.J.; Jun, A.S. A clinicopathologic series of primary graft failure after Descemet's stripping and automated endothelial keratoplasty. Ophthalmology. 2009 Apr;116(4):609-614.

    Roda, R.H.; Gallia, G.L.; Eberhart, C.G.; Weingart, J.D.; Laterra, J. Epilepsy and temporal lobe injury after skull base proton beam therapy. J Clin Neurosci. 2009 Sep;16(9):1220-1221.

    Sabet-Peyman, E.J.; Eberhart, C.G.; Janjua, K.; Shah, S.M.; Do, D.V.; Nguyen, Q.D. Persistent intermediate uveitis associated with latent manifestation of facial large B-cell non-Hodgkin lymphoma. Ocul Immunol Inflamm. 2009 Sep-Oct;17(5):322-324.

    Sun, P.; Xia, S.; Lal, B.; Eberhart, C.G.; Quinones-Hinojosa, A.; Maciaczyk, J.; Matsui, W.; Dimeco, F.; Piccirillo, S.M.; Vescovi, A.L.; Laterra, J. DNER, an epigenetically modulated gene, regulates glioblastoma-derived neurosphere cell differentiation and tumor propagation. Stem Cells. 2009 Jul;27(7):1473-1486.

    von Bueren, A.O.; Shalaby, T.; Oehler-Janne, C.; Arnold, L.; Stearns, D.; Eberhart, C.G.; Arcaro, A.; Pruschy, M.; Grotzer, M.A. RNA interference-mediated c-MYC inhibition prevents cell growth and decreases sensitivity to radio- and chemotherapy in childhood medulloblastoma cells. BMC Cancer. 2009 Jan 10;9(1):10.

    Bar, E.E.; Lin, A.; Mahairaki, V.; Matsui, W.; Eberhart, C.G. Hypoxia increases the expression of stem-cell markers and promotes clonogenicity in glioblastoma neurospheres. Am J Pathol. 2010 Sep;177(3):1491-1502.

    Cho, Y.J.; Tsherniak, A.; Tamayo, P.; Santagata, S.; Ligon, A.; Greulich, H.; Berhoukim, R.; Amani, V.; Goumnerova, L.; Eberhart, C.G.; Lau, C.C.; Olson, J.M.; Gilbertson, R.J.; Gajjar, A.; Delattre, O.; Kool, M.; Ligon, K.; Meyerson, M.; Mesirov, J.P.; Pomeroy, S.L. Integrative Genomic Analysis of Medulloblastoma Identifies a Molecular Subgroup That Drives Poor Clinical Outcome. J Clin Oncol. 2010 Dec 6.

    Engler, C.; Kelliher, C.; Spitze, A.R.; Speck, C.L.; Eberhart, C.G.; Jun, A.S. Unfolded protein response in fuchs endothelial corneal dystrophy: a unifying pathogenic pathway? Am J Ophthalmol. 2010 Feb;149(2):194-202 e192.

    Fan, X.; Khaki, L.; Zhu, T.S.; Soules, M.E.; Talsma, C.E.; Gul, N.; Koh, C.; Zhang, J.; Li, Y.M.; Maciaczyk, J.; Nikkhah, G.; Dimeco, F.; Piccirillo, S.; Vescovi, A.L.; Eberhart, C.G. NOTCH pathway blockade depletes CD133-positive glioblastoma cells and inhibits growth of tumor neurospheres and xenografts. Stem Cells. 2010 Jan;28(1):5-16.

    Gurel, B.; Ali, T.Z.; Montgomery, E.A.; Begum, S.; Hicks, J.; Goggins, M.; Eberhart, C.G.; Clark, D.P.; Bieberich, C.J.; Epstein, J.I.; De Marzo, A.M. NKX3.1 as a marker of prostatic origin in metastatic tumors. Am J Surg Pathol. 2010 Aug;34(8):1097-1105

    Jain, D.; Ebrahimi, K.B.; Miller, N.R.; Eberhart, C.G. Intraorbital meningiomas: a pathologic review using current World Health Organization criteria. Arch Pathol Lab Med. 2010 May;134(5):766-770

    Jain, D.; Singh, K.; Chirumamilla, S.; Bibat, G.M.; Blue, M.E.; Naidu, S.R.; Eberhart, C.G. Ocular MECP2 protein expression in patients with and without Rett syndrome. Pediatr Neurol. 2010 Jul;43(1):35-40.

    Johnson, M.W.; Eberhart, C.G.; Perry, A.; Tihan, T.; Cohen, K.J.; Rosenblum, M.K.; Rais-Bahrami, S.; Goldthwaite, P.; Burger, P.C. Spectrum of pilomyxoid astrocytomas: intermediate pilomyxoid tumors. The American Journal of Surgical Pathology. 2010;34(12):1783-1791.

    Lee, B.S.; Frankfort, B.J.; Eberhart, C.G.; Weinberg, R.S. Diagnosis of Intravascular Lymphoma by a Novel Biopsy Site. Ophthalmology. 2010 Oct 28.

    Nakahara, Y.; Northcott, P.A.; Li, M.; Kongkham, P.N.; Smith, C.; Yan, H.; Croul, S.; Ra, Y.S.; Eberhart, C.; Huang, A.; Bigner, D.; Grajkowska, W.; Van Meter, T.; Rutka, J.T.; Taylor, M.D. Genetic and epigenetic inactivation of Kruppel-like factor 4 in medulloblastoma. Neoplasia. 2010 Jan;12(1):20-27 Not supported through NIH funding.

    Northcott, P.A.; Korshunov, A.; Witt, H.; Hielscher, T.; Eberhart, C.G.; Mack, S.; Bouffet, E.; Clifford, S.C.; Hawkins, C.E.; French, P.; Rutka, J.T.; Pfister, S.; Taylor, M.D. Medulloblastoma Comprises Four Distinct Molecular Variants. J Clin Oncol. 2010 Dec 6.

    Pfister, S.M.; Korshunov, A.; Kool, M.; Hasselblatt, M.; Eberhart, C.; Taylor, M.D. Molecular diagnostics of CNS embryonal tumors. Acta Neuropathol. 2010 Nov;120(5):553-566.

    Raabe, E.H.; Eberhart, C.G. High-risk medulloblastoma: does c-myc amplification overrule histopathology? Pediatr Blood Cancer. 2010 Mar;54(3):344-345

    Schreck, K.C.; Taylor, P.; Marchionni, L.; Gopalakrishnan, V.; Bar, E.E.; Gaiano, N.; Eberhart, C.G. The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: a potential mechanism of therapeutic resistance. Clin Cancer Res. 2010 Dec 15;16(24):6060-6070

    Shahi, M.H.; Afzal, M.; Sinha, S.; Eberhart, C.G.; Rey, J.A.; Fan, X.; Castresana, J.S. Human hedgehog interacting protein expression and promoter methylation in medulloblastoma cell lines and primary tumor samples. J Neurooncol. 2010 Sep 19.

    Shahi, M.H.; Afzal, M.; Sinha, S.; Eberhart, C.G.; Rey, J.A.; Fan, X.; Castresana, J.S. Regulation of sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 and their epigenetic status in medulloblastoma and astrocytoma. BMC Cancer. 2010;10:614.

    Singh, K.; Rajan, K.D.; Eberhart, C. Orbital necrobiotic xanthogranuloma associated with systemic IgG4 disease. Ocul Immunol Inflamm. 2010 Oct;18(5):373-378

    Siu, I.M.; Tyler, B.M.; Chen, J.X.; Eberhart, C.G.; Thomale, U.W.; Olivi, A.; Jallo, G.I.; Riggins, G.J.; Gallia, G.L. Establishment of a human glioblastoma stemlike brainstem rodent tumor model. Journal of neurosurgeryPediatrics. 2010;6(1):92-97.

    Ying, M.; Sang, Y.; Li, Y.; Guerrero-Cazares, H.; Quinones-Hinojosa, A.; Vescovi, A.L.; Eberhart, C.G.; Xia, S.; Laterra, J. KLF9, A Differentiation-Associated Transcription Factor, Suppresses Notch1 Signaling and Inhibits Glioblastoma-Initiating Stem Cells. Stem cells. 2010;Epub 11/18/10(Journal Article).

    Yustein, J.T.; Liu, Y.C.; Gao, P.; Jie, C.; Le, A.; Vuica-Ross, M.; Chng, W.J.; Eberhart, C.G.; Bergsagel, P.L.; Dang, C.V. Induction of ectopic Myc target gene JAG2 augments hypoxic growth and tumorigenesis in a human B-cell model. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3534-3539.

    Eberhart, C.G. Molecular diagnostics in embryonal brain tumors. Brain Pathol. 2011 Jan;21(1):96-104.

    Lim, K.J.; Bisht, S.; Bar, E.E.; Maitra, A.; Eberhart, C.G. A polymeric nanoparticle formulation of curcumin inhibits growth, clonogenicity and stem-like fraction in malignant brain tumors. Cancer Biol Ther. 2011 Mar 1;11(5).
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