Images of our Work:
Yu, et al., Glycobiology 27, 657-668 (2017)
Siglec-8 (A) and Siglec-9 (B) ligands on human bronchus. Serial cross sections of human airway at the junction of the primary
and secondary bronchus were stained with Siglec-8-Fc (A) or Siglec-9-Fc (B). Images are displayed in mirror image to emphasize
differential siglec ligand distributions. Arrowheads: airway epithelium; arrows: submucosal glands; asterisks: cartilage. Scale bar, 200 Ám.
Jia, et al., J Allergy Clin Immunol 135, 799-810 (2015)
Human tracheal histology (H&E, left panels) stained for Siglec-8 ligands (right top) and Siglec-9 ligands (right bottom).
Siglec-8 ligands are found in serosal cells and ducts of the submucosal glands, whereas Siglec-9 ligands are in
serosal cells, ducts, connective tissue, and (light staining) mucosal cells.
Mehta, et al., ACS Chemical Neuroscience 1, 215-222 (2010)
Dorsal root ganglion neurites (labeled with anti-tubulin
antibody) were analyzed for length and integrity. Healthy neurites
are green and segmented (degenerating) neurites are red. The chemotherapeutic agent vincristine causes degeneration
(center panel). Myelin protects neurites from vincristine toxicity (right panel).
Mehta, et al., J Biol Chem 282, 27875-27886 (2007)
Left: Dorsal root ganglion neurons labelled with anti-tubulin
antibody. Top, control; Bottom, inhibited by myelin-associated glycoprotein
Right: MAG inhibits axon outgrowth via two independent pathways.
Gong, et al., Brain 125, 2491-2506 (2002)
Dorsal root ganglia triple-labelled with anti-ganglioside mAbs (green), neurofilament (red) and IB-4 (blue).
Co-localization of three labels is also shown (Merged). The mAbs included in this figure are shown on the left. Bar = 20 Ám.
Lunn, et al., J. Neurochem. 75, 404-412 (2000)
Gangliosides on neurons in cell culture. Rat cerebellar
granule neurons immunostained with anti-GD1a antibody (left).
Corresponding Hoffman modulation contrast image (right). Asterisk denotes non-neuronal cells, which fail to stain.
Sheikh, et al., Proc. Natl. Acad. Sci. USA 96, 7532-7537 (1999)
Axon degeneration (left) and demyelination (right) in "complex ganglioside" knockout mice
Tiemeyer, et al., J. Biol. Chem. 265, 11990-11999 (1990).
Binding of radiolabeled ganglioside ligand to receptors in the rat brain. Left: Rat brain section stained
with cresyl violet. Right: Adjacent section treated with 125I-GT1b-BSA, washed, and subjected to autoradiography.
Note prominent white matter but not gray matter distribution of ganglioside receptors.
Weisz and Schnaar, J. Cell Biol. 115, 485-493 (1991)
Confocal immunolocalization of the hepatic lectin on the surface of rat hepatocytes
adhering to galactose-derivatized surfaces
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