Images of our Work:

  Zhang G-L and Porter MJ (2021)

Delivery of photoaffinity & click tagged ganglioside GM1 to human epithelial cells in culture.
Red: Alexa 555 click tag; Blue: nuclei

  Gonzalez-Gil, et al., J Allergy Clin Immunol (2020)

Siglec-8 ligand on human airways is a glycoform of DMBT1. Serial cross section of human airway
submucosal gland was double-stained with Siglec-8-Fc (violet) and anti-DMBT1 antibody (green). The
overlay (right) shows the molecules are congruent. Scale bar, 50 Ám. The combined image includes
a marker for cell nuclei (blue)

  Yu, et al., Glycobiology 27, 657-668 (2017)

Siglec-8 (A) and Siglec-9 (B) ligands on human bronchus. Serial cross sections of human airway
at the junction of the primary and secondary bronchus were stained with Siglec-8-Fc (A)
or Siglec-9-Fc (B). Images are displayed in mirror image to emphasize differential siglec ligand
distributions. Arrowheads: airway epithelium; arrows: submucosal glands; asterisks: cartilage.
Scale bar, 200 Ám.

  Jia, et al., J Allergy Clin Immunol 135, 799-810 (2015)

Human tracheal histology (H&E, left panels) stained for Siglec-8 ligands (right top) and Siglec-9 ligands (right bottom).
Siglec-8 ligands are found in serosal cells and ducts of the submucosal glands, whereas Siglec-9 ligands are in
serosal cells, ducts, connective tissue, and (light staining) mucosal cells.

  Mehta, et al., ACS Chemical Neuroscience 1, 215-222 (2010)

Dorsal root ganglion neurites (labeled with anti-tubulin antibody) were analyzed for length and integrity. Healthy neurites
are green and segmented (degenerating) neurites are red. The chemotherapeutic agent vincristine causes degeneration
(center panel). Myelin protects neurites from vincristine toxicity (right panel).

    Mehta, et al., J Biol Chem 282, 27875-27886 (2007)

Left: Dorsal root ganglion neurons labelled with anti-tubulin antibody. Top, control; Bottom, inhibited by myelin-associated
glycoprotein (MAG).Right: MAG inhibits axon outgrowth via two independent pathways.

                      Gong, et al., Brain 125, 2491-2506 (2002)

Dorsal root ganglia triple-labelled with anti-ganglioside mAbs (green), neurofilament (red) and IB-4 (blue).
Co-localization of three labels is also shown (Merged). The mAbs included in this figure are shown on the left. Bar = 20 Ám.

  Lunn, et al., J. Neurochem. 75, 404-412 (2000)

Gangliosides on neurons in cell culture. Rat cerebellar granule neurons immunostained with anti-GD1a antibody (left).
Corresponding Hoffman modulation contrast image (right). Asterisk denotes non-neuronal cells, which fail to stain.

Sheikh, et al., Proc. Natl. Acad. Sci. USA 96, 7532-7537 (1999)

Axon degeneration (left) and demyelination (right) in "complex ganglioside" knockout mice

Tiemeyer, et al., J. Biol. Chem. 265, 11990-11999 (1990).

Binding of radiolabeled ganglioside ligand to receptors in the rat brain. Left: Rat brain section stained
with cresyl violet. Right: Adjacent section treated with 125I-GT1b-BSA, washed, and subjected to autoradiography.
Note prominent white matter but not gray matter distribution of ganglioside receptors.

  Weisz and Schnaar, J. Cell Biol. 115, 485-493 (1991)

Confocal immunolocalization of the hepatic lectin on the surface of rat hepatocytes
adhering to galactose-derivatized surfaces

Return to HOME PAGE or go to:

Research Goals

Recent Publications

The Schnaar Lab

Current Projects

Biographical Information

Schnaar Lab Alumni