Researchers in the Laboratory for Fetal and Neonatal Organ Regeneration in the Department of Surgery at the Johns Hopkins School of Medicine are studying whether cellular reprogramming, stem cells, and ex vivo modeling can be applied to improve organ regeneration in pediatric surgical patients.
To execute these aims, the lab collaborates with developmental biologists and biomedical engineers throughout the country and employs cutting-edge molecular strategies and pre-clinical animal models.
The mission and interest of the neuroengineering and Biomedical Instrumentation Lab is to develop novel instrumentation and technologies to study the brain at several levels--from single cell to the whole brain--with the goal of translating the work into practical research and clinical applications.
Our personnel include diverse, independent-minded and entrepreneurial students, post docs, and research faculty who base their research on modern microfabrication, stem cell biology, electrophysiology, signal processing, image processing, and integrated circuit design technologies.
Researchers in the Pankaj Jay Pasricha Lab are interested in the molecular mechanisms of visceral pain and restoration of enteric neural function with novel strategies, including neural stem cell transplants. Recent research has focused on the enteric nervous system and gut-brain axis, and the complexity of pain in chronic pancreatitis. Another recent study indicates that patients with underlying small intestinal bacterial overgrowth have significant delays in small bowel transit time as compared to those without, while another explored the safety and efficacy of carbon dioxide cryotherapy for treatment of neoplastic Barrett's esophagus.
The Reeves Lab complements genetic analyses in human beings with the creation and characterization of mouse models to understand why and how gene dosage imbalance disrupts development in Down syndrome (DS). These models then provide a basis to explore therapeutic approaches to amelioration of DS features. We use chromosome engineering in embryonic stem cells (ES) to create defined dosage imbalance in order to localize the genes contributing to these anomalies and to directly test hypotheses concerning Down syndrome "critical regions" on human chromosome 21.
The Richard J. Jones Lab studies normal and cancerous stem cells in order to make clinical improvements in areas such as blood and marrow transplantation (BMT). We discovered one of the most common stem-cell markers, Aldefluor, which identifies cells based on their expression of aldehyde dehydrogenase 1 (ALDH1), and have used this marker to detect and characterize normal stem cells and cancer stem cells from many hematologic malignancies. We also developed post-transplant cyclophosphamide and effective related haploidentical BMT.
The goal of the Singh Lab is to cure retinal degeneration due to genetic disease in patients. There are many retinal diseases such as Stargardts, Macular Degeneration, and Retinitis Pigmentosa, that are currently incurable. These diseases damage and eventually eliminate photoreceptors in the retina. The lab's aim is to take healthy photoreceptors derived from stem cells and transplant them into the patient’s retina to replace the lost photoreceptors. The transplanted photoreceptors are left to mature, make connections with the recipient’s remaining retina, and restore vision. Further, the lab is most interested in the cone-photoreceptor rich region of the macula, which is the central zone of the human retina, enabling high-acuity vision for tasks such as facial recognition and reading.
Founded in the late 1980s, our Lab has been exploring the fundamental mechanisms of neural responses to traumatic and degenerative signals as well as mechanisms of neural repair. Our current interests include: traumatic brain injury and models; mechanisms and treatments of traumatic axonopathies; molecular neuropathology of traumatic brain injury; induced pluripotent stem cells as models of disease.
The Yarema Lab uses chemical biology, molecular and cell biology, and materials science methods to study and manipulate glycosylation. The goal of our research is to better understand human disease while furthering carbohydrate-based therapies.
Our laboratory's research goals are to (1) Develop sugar analogs into viable and versatile drug candidates, (2) Apply metabolic glycoengineering to tissue engineering and stem cell research, (3) Use non-invasive magnetic stimuli to probe the effects of glycoengineering (and also to treat neurological disorders), and (4) Extend our sugar-based drug candidates into animal models and the clinic
The Wang lab focuses on the signals that direct the differentiation of pluripotent stem cells, such as induced-pluripotent stem (iPS) cells, into hematopoietic and cardiovascular cells. Pluripotent stem cells hold great potential for regenerative medicine. Defining the molecular links between differentiation outcomes will provide important information for designing rational methods of stem cell manipulation.
The Zambidis Labratory studies the formation of pluripotent stem cells and the subsequent hematopoietic, endothelial and cardiac differentiation, as well as the potential therapeutic uses of pluripotent stem cell-derived cells.