Skip Navigation

Find a Research Lab

Research Lab Results for proteins

Displaying 21 to 40 of 41 results
Results per page:
  • O'Rourke Lab

    Lab Website
    Principal Investigator:
    Brian O'Rourke, Ph.D.
    Medicine

    The O’Rourke Lab uses an integrated approach to study the biophysics and physiology of cardiac ...cells in normal and diseased states.

    Research in our lab has incorporated mitochondrial energetics, Ca2+ dynamics, and electrophysiology to provide tools for studying how defective function of one component of the cell can lead to catastrophic effects on whole cell and whole organ function. By understanding the links between Ca2+, electrical excitability and energy production, we hope to understand the cellular basis of cardiac arrhythmias, ischemia-reperfusion injury, and sudden death.

    We use state-of-the-art techniques, including single-channel and whole-cell patch clamp, microfluorimetry, conventional and two-photon fluorescence imaging, and molecular biology to study the structure and function of single proteins to the intact muscle. Experimental results are compared with simulations of computational models in order to understand the findings in the context of the system as a whole.

    Ongoing studies in our lab are focused on identifying the specific molecular targets modified by oxidative or ischemic stress and how they affect mitochondrial and whole heart function.

    The motivation for all of the work is to understand
    • how the molecular details of the heart cell work together to maintain function and
    • how the synchronization of the parts can go wrong

    Rational strategies can then be devised to correct dysfunction during the progression of disease through a comprehensive understanding of basic mechanisms.

    Brian O’Rourke, PhD, is a professor in the Division of Cardiology and Vice Chair of Basic and Translational Research, Department of Medicine, at the Johns Hopkins University.
    view more

    Research Areas: biophysics, ischemia-reperfusion injury, imaging, electrophysiology, cardiovascular, arrhythmia, physiology, sudden cardiac death, molecular biology, cardiac cells
  • Paul Worley Lab

    Lab Website
    Principal Investigator:
    Paul Worley, M.D.
    Neuroscience

    The Paul Worley Lab examines the molecular basis of learning and memory. In particular, we clon...ed a set of immediate early genes (IEGs) that are rapidly transcribed in neurons involved in information processing, and that are essential for long term memory. IEG proteins can directly modify synapses and provide insight into cellular mechanisms that support synapse-specific plasticity. view more

    Research Areas: synaptic plasticity, neurons, memory, learning, immediate early genes
  • Retrovirus Laboratory

    Principal Investigator:
    Janice Clements, Ph.D.
    Molecular and Comparative Pathobiology

    Research in the Retrovirus Laboratory focuses on the molecular virology and pathogenesis of len...tivirus infections. In particular, we study the simian immunodeficiency virus (SIV) to determine the molecular basis for the development of HIV CNS, pulmonary and cardiac disease.

    Research projects include studies of viral molecular genetics and host cell genes and proteins involved in the pathogenesis of disease. We are also interested in studies of lentivirus replication in macrophages and astrocytes and their role in the development of disease. These studies have led us to identify the viral genes that are important in neurovirulence of SIV and the development of CNS disease including NEF and the TM portion of ENV. The mechanisms of the action of these proteins in the CNS are complex and are under investigation. We have also developed a rapid, consistent SIV/macaque model in which we can test the ability of various antiviral and neuroprotective agents to reduce the severity of CNS and pulmonary disease.
    view more

    Research Areas: HIV, genomics, pulmonology, SIV, cardiology, lentivirus
  • Ronald Schnaar Lab

    The Ronald Schnaar Lab is involved in the rapidly expanding field of glycobiology, which studie...s cell surface glycans, lectins, and their roles in cell physiology.

    Current projects in our lab study include (1) Glycans and glycan-binding proteins in inflammatory lung diseases, (2) Ganglioside function in the brain, and (3) HIV-Tat and HIV-associated neurocognitive disorders.
    view more

    Research Areas: cell physiology, HIV, neurocognitive disorders, glycobiology
  • Saraswati Sukumar Lab

    Lab Website
    Principal Investigator:
    Saraswati Sukumar, Ph.D.
    Oncology

    Our lab is focused on using comprehensive gene expression, methylation and sequencing and metab...olomics analysis to identify alterations in breast cancer, and exploiting these for early detection and therapy. Among deferentially expressed genes, our lab has focused on the HOX genes. HOX genes are intimately involved in the development of resistance to both chemotherapy and to agents targeting the estrogen receptor. Our work explores the alternate pathways that are activated by HOX proteins leading to this resistance and novel treatments to overcome resistance in both tissue culture and xenograft models. In addition, epigenetically silenced genes and a metabolic reprogramming in tumors also trigger novel early detection and therapeutic strategies. We are testing the utility of differentiation therapy through reactivating RAR-beta in breast cancer using histone deacetylase inhibitors with great success. Also, we are targeting enzymes involved in gluconeogenesis and glycolysis with small molecule FDA-approved antimetabolites to achieve antitumor effects. view more

    Research Areas: breast cancer, genetics
  • Sean Taverna Laboratory

    The Taverna Laboratory studies histone marks, such as lysine methylation and acetylation, and h...ow they contribute to an epigenetic/histone code that dictates chromatin-templated functions like transcriptional activation and gene silencing. Our lab uses biochemistry and cell biology in a variety of model organisms to explore connections between gene regulation and proteins that write and read histone marks, many of which have clear links to human diseases like leukemia and other cancers. We also investigate links between small RNAs and histone marks involved in gene silencing. view more

    Research Areas: biochemistry, histone marks, cell biology, leukemia, cancer, epigenetics, eukaryotic cells, gene silencing, RNA
  • Seth Blackshaw Lab

    Lab Website
    Principal Investigator:
    Seth Blackshaw, Ph.D.
    Neuroscience

    The Seth Blackshaw Lab uses functional genomics and proteomics to rapidly identify the molecula...r mechanisms that regulate cell specification and survival in both the retina and hypothalamus. We have profiled gene expression in both these tissues, from the start to the end of neurogenesis, characterizing the cellular expression patterns of more than 1,800 differentially expressed transcripts in both tissues. Working together with the lab of Heng Zhu in the Department of Pharmacology, we have also generated a protein microarray comprised of nearly 20,000 unique full-length human proteins, which we use to identify biochemical targets of developmentally important genes of interest. view more

    Research Areas: retina, central nervous system, biochemistry, hypothalamus, proteomics, genomics
  • Shanthini Sockanathan Laboratory

    Lab Website

    The Shanthini Sockanathan Laboratory uses the developing spinal cord as our major paradigm to d...efine the mechanisms that maintain an undifferentiated progenitor state and the molecular pathways that trigger their differentiation into neurons and glia. The major focus of the lab is the study of a new family of six-transmembrane proteins (6-TM GDEs) that play key roles in regulating neuronal and glial differentiation in the spinal cord. We recently discovered that the 6-TM GDEs release GPI-anchored proteins from the cell surface through cleavage of the GPI-anchor. This discovery identifies 6-TM GDEs as the first vertebrate membrane bound GPI-cleaving enzymes that work at the cell surface to regulate GPI-anchored protein function. Current work in the lab involves defining how the 6-TM GDEs regulate cellular signaling events that control neuronal and glial differentiation and function, with a major focus on how GDE dysfunction relates to the onset and progression of disease. To solve these questions, we use an integrated approach that includes in vivo models, imaging, molecular biology, biochemistry, developmental biology, genetics and behavior. view more

    Research Areas: glia, biochemistry, neurons, imaging, developmental biology, genomics, spinal cord, behavior, molecular biology
  • Solomon Snyder Laboratory

    Lab Website

    Information processing in the brain reflects communication among neurons via neurotransmitters.... The Solomon Snyder Laboratory studies diverse signaling systems including those of neurotransmitters and second messengers as well as the actions of drugs upon these processes. We are interested in atypical neurotransmitters such as nitric oxide (NO), carbon monoxide (CO), and the D-isomers of certain amino acids, specifically D-serine and D-aspartate. Our discoveries are leading to a better understanding of how certain drugs for Parkinson's disease and Hungtington's disease interact with cells and proteins. Understanding how other second messengers work is giving us insight into anti-cancer therapies. view more

    Research Areas: Huntington's disease, amino acids, neurotransmitters, brain, cancer, nitric oxide, drugs, carbon monoxide, Parkinson's disease, nervous system
  • Steven Claypool Lab

    Lab Website
    Principal Investigator:
    Steven Claypool, Ph.D.
    Physiology

    Research in the Claypool Lab is focused on defining how lipids and membrane proteins interact t...o establish and maintain normal mitochondrial function and how derangements in this complex relationship result in pathophysiology. We have demonstrated that yeast lacking tafazzin recapitulates all of the phospholipid abnormalities observed in human patients and many of the mitochondrial defects.

    Another major project in our lab focuses on the mitochondrial ADP/ATP carrier that is required for oxidative phosphorylation. Researchers are studying how these novel interactions help establish normal mitochondrial function, the biochemical details of these associations, and whether disturbances in these assemblies can contribute to mitochondrial dysfunction.
    view more

    Research Areas: biochemistry, proteomics, lipids, yeast, mitochondria, oxidative phosphorylation
  • Susan Michaelis Lab

    Principal Investigator:
    Susan Michaelis, Ph.D.
    Cell Biology

    The Michaelis Laboratory's research goal is to dissect fundamental cellular processes relevant ...to human health and disease, using yeast and mammalian cell biology, biochemistry and high-throughput genomic approaches. Our team studies the cell biology of lamin A and its role in the premature aging disease Hutchinson-Gilford progeria syndrome (HGPS). Other research focuses on the core cellular machinery involved in recognition of misfolded proteins. Understanding cellular protein quality control machinery will ultimately help researchers devise treatments for protein misfolding diseases in which degradation is too efficient or not enough. view more

    Research Areas: biochemistry, cell biology, protein folding, lamin A, aging, genomics, Hutchinson-Gilford progeria syndrome, yeast
  • Tamara O'Connor Lab

    The O'Connor Lab studies the molecular basis of infectious disease using Legionella pneumophila... pathogenesis as a model system.

    We are looking at the network of molecular interactions acting at the host-pathogen interface. Specifically, we use L. pneumophila pathogenesis to examine the numerous mechanisms by which an intracellular bacterial pathogen can establish infection, how it exploits host cell machinery to accomplish this, and how individual proteins and their component pathways coordinately contribute to disease.

    We are also studying the role of environmental hosts in the evolution of human pathogens. Using genetics and functional genomics, we compare and contrast the repertoires of virulence proteins required for growth in a broad assortment of hosts, how the network of molecular interactions differs between hosts, and the mechanisms by which L. pneumophila copes with this variation.
    view more

    Research Areas: infectious disease, Legionella pneumophila, genomics, pathogenesis, molecular biology
  • The Sun Laboratory

    Lab Website
    Principal Investigator:
    Shuying Sun, Ph.D.
    Pathology

    The nervous system has extremely complex RNA processing regulation. Dysfunction of RNA metaboli...sm has emerged to play crucial roles in multiple neurological diseases. Mutations and pathologies of several RNA-binding proteins are found to be associated with neurodegeneration in both amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). An alternative RNA-mediated toxicity arises from microsatellite repeat instability in the human genome. The expanded repeat-containing RNAs could potentially induce neuron toxicity by disrupting protein and RNA homeostasis through various mechanisms.

    The Sun Lab is interested in deciphering the RNA processing pathways altered by the ALS-causative mutants to uncover the mechanisms of toxicity and molecular basis of cell type-selective vulnerability. Another major focus of the group is to identify small molecule and genetic inhibitors of neuron toxic factors using various high-throughput screening platforms. Finally, we are also highly interested in developing novel CRISPR technique-based therapeutic strategies. We seek to translate the mechanistic findings at molecular level to therapeutic target development to advance treatment options against neurodegenerative diseases.
    view more

    Research Areas: ALS, neurodegeneration, RNA
  • Wei Dong Gao Lab

    Lab Website

    Work in the Wei Dong Gao Lab primarily focuses on heart failure and defining molecular and cell...ular mechanisms of contractile dysfunction. We use molecular biology and proteomic techniques to investigate the changes that myofilament proteins undergo during heart failure and under drug therapy. We're working to determine the molecular nature of nitroxyl (HNO) modification of tropomyosin. view more

    Research Areas: heart disease, contractile dysfunction, heart failure, cardiovascular diseases, molecular biology
  • William B. Guggino Lab

    Lab Website
    Principal Investigator:
    William Guggino, Ph.D.
    Physiology

    Work in the William B. Guggino Lab focuses on the structure of the cystic fibrosis transmembran...e conductance regulator (CFTR) and water channels; the molecular structure of transport proteins in epithelial cell membranes; and gene therapies to treat cystic fibrosis (CF) patients. We are also working to identify CF’s specific defect in chloride channel regulation. One recent study showed that insulin-like growth factor 1 (IGF-1) enhances the protein expression of CFTR. view more

    Research Areas: cell biology, cystic fibrosis, kidney diseases, gene therapy, ion channels
  • Zachara Lab

    Lab Website

    Elevation of O-GlcNAc levels modulates numerous pathways in a manner consistent with increased ...cell survival, including the expression of heat shock proteins. The Zachara Lab's goal is to understand the O-GlcNAc regulated stress response, how this can be manipulated to improve patient outcome and how this response is misregulated in disease. view more

    Research Areas: stress response, proteomics, O-GlcNAc, heat shock proteins
  • Zhaozhu Qiu Laboratory

    Lab Website
    Principal Investigator:
    Zhaozhu Qiu, Ph.D.
    Neuroscience
    Physiology

    Ion channels are pore-forming membrane proteins gating the flow of ions across the cell membran...e. Among their many functions, ion channels regulate cell volume, control epithelial fluid secretion, and generate the electrical impulses in our brain. The Qiu Lab employs a multi-disciplinary approach including high-throughput functional genomics, electrophysiology, biochemistry, and mouse genetics to discover novel ion channels and to elucidate their role in health and disease. view more

    Research Areas: ion channel, neurological disease, electrophysiology, functional genomics, sensory neuroscience
  1. 1
  2. 2
  3. 3
Create lab profile
Edit lab profile
back to top button