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Displaying 1 to 5 of 5 results for protein expression

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  • Cardiac Bioelectric Systems Laboratory

    The Cardiac Bioelectric Systems Laboratory research focuses on both the physiological and pathophysiological function of cardiac cells at a multicellular, syncytial level. We use cell culture models in a manner akin to mathematical models in which elements of the model can be designed, synthesized or controlled. Our traditional approach consists of cultured, confluent monolayers of cardiac cells that number in the tens of thousands to a million. These cell monolayers can be engineered in terms of their tissue architecture, cell type, protein expression and microenvironment, and have been used to study clinically relevant phenomena in the heart that include electrical stimulation, electrical propagation, arrhythmia and cell therapy.

    Research Areas: bioelectric systems, arrhythmia, cell therapy, cardiology

    Lab Website

    Principal Investigator

    Leslie Tung, Ph.D.

    Department

    Biomedical Engineering

  • Caren L. Freel Meyers Laboratory

    The long-term goal of the Caren L. Freel Meyers Laboratory is to develop novel approaches to kill human pathogens, including bacterial pathogens and malaria parasites, with the ultimate objective of developing potential therapeutic agents.

    Toward this goal, we are pursuing studies of bacterial isoprenoid biosynthetic enzymes comprising the methylerythritol phosphate (MEP) pathway essential in many human pathogens. Studies focus on understanding mechanism and regulation in the pathway toward the development of selective inhibitors of isoprenoid biosynthesis. Our strategies for creating new anti-infective agents involve interdisciplinary research in the continuum of organic, biological and medicinal chemistry. Molecular biology, protein expression and biochemistry, and synthetic chemistry are key tools for our research.

    Research Areas: bacterial pathogens, biochemistry, enzymes, infectious disease, protein expression, synthetic chemistry, isoprenoid biosynthesis, malaria, pharmacology, chemistry, molecular biology

  • Mass Spectrometry Core

    The Mass Spectrometry Core identifies and quantifies proteins that change expression in well-characterized protein fractions from cancerous cells or tissues. This includes identifying and quantifying changes in binding partners and post-translational modifications. Column chromatography and gel electrophoresis-based one and two-dimensional separations of protein complexes coupled to mass spectrometry are used. Techniques such as difference gel electrophoresis (DIGE), isobaric tag for relative and absolute quantitation (iTRAQ) and 18O-labeling as well as non-labeling methods (MudPit, multi-dimensional protein identification technology) are available for quantifying relative differences in protein expression and post-translational modifications. We developed methods to detect post-translational modifications such as LCMS methods to accurately determine the intact mass of proteins, selective fluorescent labeling of S-nitrosothiols (S-FLOS) to detect nitrosated cysteines in proteins, and i...on mapping methods to map post-translational modifications that produce a signature mass or mass difference when the modified peptide is fragmented. view more

    Research Areas: mass spectrometry, proteomics, cancer

  • The Arking Lab

    The Arking Lab studies the genomics of complex human disease, with the primary goal of identifying and characterizing genetics variants that modify risk for human disease. The group has pioneered the use of genome-wide association studies (GWAS), which allow for an unbiased screen of virtually all common genetic variants in the genome. The lab is currently developing improved GWAS methodology, as well as exploring the integration of additional genome level data (RNA expression, DNA methylation, protein expression) to improve the power to identify specific genetic influences of disease.

    The Arking Lab is actively involved in researching:
    • autism, a childhood neuropsychiatric disorder
    • cardiovascular genomics, with a focus on electrophysiology and sudden cardiac death (SCD)
    • electrophysiology is the study of the flow of ions in biological tissues

    Dan E. Arking, PhD, is an associate professor at the McKusick-Nathans Institute of Genetic Medicine and Department of Medicine, D...ivision of Cardiology, Johns Hopkins University. view more

    Research Areas: autism, genetics, aging, cardiovascular diseases, sudden cardiac death

    Principal Investigator

    Dan Arking, Ph.D.

    Department

    Medicine

  • William B. Guggino Lab

    Work in the William B. Guggino Lab focuses on the structure of the cystic fibrosis transmembrane conductance regulator (CFTR) and water channels; the molecular structure of transport proteins in epithelial cell membranes; and gene therapies to treat cystic fibrosis (CF) patients. We are also working to identify CF’s specific defect in chloride channel regulation. One recent study showed that insulin-like growth factor 1 (IGF-1) enhances the protein expression of CFTR.

    Research Areas: cell biology, cystic fibrosis, kidney diseases, gene therapy, ion channels

    Lab Website

    Principal Investigator

    William Guggino, Ph.D.

    Department

    Physiology

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