Skip Navigation

Find a Research Lab

Research Lab Results for RNA

Displaying 1 to 10 of 16 results
Results per page:
  • Balagopal Lab - Viral Hepatitis and Translational Virology

    Principal Investigator:
    Ashwin Balagopal, M.D.
    Medicine

    The Balagopal Lab has adapted high-resolution tools to study viruses in situ. Specifically, we ...were the first to quantify hepatitis C virus (HCV) infection in single hepatocytes by developing single-cell laser capture microdissection (scLCM) and integrating this tool with highly sensitive quantitative real-time PCR. We reported that HCV infects a minority of hepatocytes that are found in geospatial clusters. More recently, we (PIs Balagopal and Thio) integrated scLCM with droplet digital PCR (ddPCR) to reveal the first observations of hepatitis B virus (HBV) infection at single cell resolution in the liver. We found that HBV infects nearly all hepatocytes prior to antiviral therapy. However, during antiviral therapy, HBV infection is diminished while viral transcription is markedly attenuated.

    Our lab has also focused on HIV-1 infection and immune activation for over a decade. Most recently, we have studied type 1 interferon responses to HIV-1 using RNA sequencing (RNAseq). Using this technology, we identified novel interferon-stimulated genes (ISGs) that are associated with HIV-1 restriction in vivo.
    view more

    Research Areas: HIV-1, hepatitis B, hepatitis C
  • Beer Lab

    Lab Website

    The goal of research in the Beer Lab is to understand how gene regulatory information is encode...d in genomic DNA sequence. Our work uses functional genomics DNase-seq, ChIP-seq, RNA-seq, and chromatin state data to computationally identify combinations of transcription factor binding sites that operate to define the activity of cell-type specific enhancers. We are currently focused on improving SVM methodology by including more general sequence features and constraints predicting the impact of SNPs on enhancer activity (delta-SVM) and GWAS association for specific diseases, experimentally assessing the predicted impact of regulatory element mutation in mammalian cells, systematically determining regulatory element logic from ENCODE human and mouse data, and using this sequence based regulatory code to assess common modes of regulatory element evolution and variation. view more

    Research Areas: computational biology, biomedical engineering, DNA, genomics, RNA
  • James Hamilton Lab

    Principal Investigator:
    James Hamilton, M.D.
    Medicine

    The main research interests of the James Hamilton Lab are the molecular pathogenesis of hepatoc...ellular carcinoma and the development of molecular markers to help diagnose and manage cancer of the liver. In addition, we are investigating biomarkers for early diagnosis, prognosis and response to various treatment modalities. Results of this study will provide a molecular classification of HCC and allow us to identify targets for chemoprevention and treatment. Specifically, we extract genomic DNA and total RNA from liver tissues and use this genetic material for methylation-specific PCR (MSP), cDNA microarray, microRNA microarray and genomic DNA methylation array experiments. view more

    Research Areas: Copper homeostasis, Wilson's disease, cancer, molecular genetics, Early detection biomarker discovery for hepatocellular carcinoma, genomics, pathogenesis, liver injury, liver diseases, regulation of lipid metabolism, hepatocellular carcinoma, Pathogenesis of Liver fibrosis and cancer
  • Ken Witwer Laboratory

    Principal Investigator:
    Kenneth Witwer, Ph.D.
    Molecular and Comparative Pathobiology

    The Ken Witwer Laboratory investigates extracellular vesicles and RNA in the context of HIV inf...ection and inflammatory disease. We are also actively assessing the effects of diet on extracellular RNA as a potential therapeutic approach. view more

    Research Areas: extracellular vesicles, HIV, inflammation, RNA
  • Liliana Florea Lab

    Principal Investigator:
    Liliana Florea, Ph.D., M.Sc.
    Medicine

    Research in the Liliana Florea Lab applies computational techniques toward modeling and problem... solving in biology and genetic medicine. We work to develop computational methods for analyzing large-scale sequencing data to help characterize molecular mechanisms of diseases. The specific application areas of our research include genome analysis and comparison, cDNA-to-genome alignment, gene and alternative splicing annotation, RNA editing, microbial comparative genomics, miRNA genomics and computational vaccine design. Our most recent studies seek to achieve accurate and efficient RNA-seq correction and explore the role of HCV viral miRNA in hepatocellular carcinoma. view more

    Research Areas: evolutionary genomics, vaccines, carcinoma, cancer, genomics, bioinformatics, RNA, comparative genomics
  • Loyal Goff Laboratory

    Lab Website
    Principal Investigator:
    Loyal Goff, Ph.D.
    Neuroscience

    The Loyal Goff Laboratory seeks to answer a fundamental biological question: How is the genome... properly interpreted to coordinate the diversity of cell types observed during neuronal development? We are focused on the acquisition of specific cellular identities in neuronal development and identifying the molecular determinants responsible for proper brain development. Using novel experimental approaches for the enrichment and purification of specific neuronal cell types and recent technological advances in single-cell RNA sequencing, we can discover and explore the cellular factors that contribute to neuronal cell fate decisions during mammalian brain development. view more

    Research Areas: brain, neuronal development, genomics, RNA
  • Michael Caterina Lab

    The Caterina lab is focused on dissecting mechanisms underlying acute and chronic pain sensatio...n. We use a wide range of approaches, including mouse genetics, imaging, electrophysiology, behavior, cell culture, biochemistry and neuroanatomy to tease apart the molecular and cellular contributors to pathological pain sensation. A few of the current projects in the lab focus on defining the roles of specific subpopulations of neuronal and non-neuronal cells to pain sensation, defining the role of RNA binding proteins in the development and maintenance of neuropathic pain, and understanding how rare skin diseases known as palmoplantar keratodermas lead to severe pain in the hands and feet. view more

    Research Areas: biophysics, biochemistry, proteomics, inflammation, pain
  • Mihaela Pertea Lab

    Lab Website

    The Mihaela Pertea Lab develops computational tools for RNA sequence analysis, gene finding, sp...lice-site prediction and sequence-motif finding. Previous research projects led to the development of open-source software systems related to finding genes. view more

    Research Areas: computational biology, DNA, genomics, RNA
  • Ryuya Fukunaga Lab

    The Fukunaga Lab uses multidisciplinary approaches to understand the cell biology, biogenesis a...nd function of small silencing RNAs from the atomic to the organismal level.

    The lab studies how small silencing RNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and piwi-interacting RNAs (piRNAs), are produced and how they function. Mutations in the small RNA genes or in the genes involved in the RNA pathways cause many diseases, including cancers. We use a combination of biochemistry, biophysics, fly genetics, cell culture, X-ray crystallography and next-generation sequencing to answer fundamental biological questions and also potentially lead to therapeutic applications to human diseases.
    view more

    Research Areas: biophysics, biochemistry, cell biology, cell culture, genomics, RNA
  1. 1
  2. 2
Create lab profile
Edit lab profile
back to top button