The Johns Hopkins Russell H. Morgan Department of Radiology and Radiological Science is internationally recognized for its leadership in innovation and research in all aspects of molecular, functional and anatomic imaging. The service centers and cores within the Department provide state-of-the-art multi-modality imaging infrastructure to support and advance the research needs of Johns Hopkins faculty. These service centers are also available to investigators outside of Johns Hopkins.
Services available through these centers are offered for both clinical and pre-clinical research dedicated to advancing medicine through groundbreaking discoveries on medical imaging techniques, disease treatments, and interventional procedures.
The Johns Hopkins Applied Imaging Mass Spectrometry (AIMS) Core/Service Center provides rapid matrix-assisted laser desorption/ionization (MALDI) imaging at high spatial resolution, which includes sample preparation, on-tissue digests and derivatizations, and data analysis.
Spatially resolved MALDI imaging measurements are directly taken from a frozen or formalin-fixed paraffin-embedded (FFPE) tissue section without destroying it. MALDI imaging combines mass spectrometric analyses of biomolecules with simultaneous histological evaluation to analyze intact proteins, peptides and tryptic peptides (on-tissue tryptic digest), N-glycans (on-tissue PNGase digest), lipids, metabolites and drug molecules in a spatially resolved manner. The Johns Hopkins AIMS Core provides comprehensive service in MALDI tissue imaging to investigators at Johns Hopkins and outside.
- Bruker Rapiflex MALDI TOF/TOF instrument for high-throughput MALDI imaging
- HTX M5 sprayer for accurate robotic spraying of enzymes and matrices
- Leica cryostat for MALDI imaging-compatible cryosectioning
- Slide scanner for histology and immunohistochemistry co-registered with MALDI imaging
- High-end workstation with SCiLS lab for data analysis
- MALDI imaging compatible cryosectioning in gelatin and other MALDI compatible cryomedia
- MALDI imaging sample preparation of formalin-fixed, paraffin-embedded tissue sections
- On-tissue digestions including for tryptic peptides and glycans
- On-tissue derivatizations for metabolite imaging
- Robotic matrix application with HTX M5 sprayer
- High-throughput multiplexed MALDI imaging of up to 5,000 biomolecules at once
- High spatial resolution MALDI imaging of 20 micron pixel size or better
- MALDI imaging covering maximum area of regular microscopy slide (75 millimeters by 25 millimeters)
- Customized development of MALDI imaging protocols
- Targeted MALDI imaging of drugs, drug metabolites, imaging agents, contrast agents or other agents
- Discovery MALDI imaging of metabolites, lipids, peptides, intact proteins, tryptic peptides and glycans
- On tissue MS/MS for analyte identification in imaging or profiling mode
- Data analysis: segmentation analysis, pathology-guided analysis and statistical analysis with dedicated software package (SCiLS Lab)
For more information, please contact:
Dr. Kristine Glunde
The Russell H. Morgan Department of Radiology and Radiological Science
The Johns Hopkins University School of Medicine
Office: Traylor Building, Room 203, Rutland Avenue, Baltimore, Maryland 21205
AIMS Core: Cancer Research Building II, Room LB03D/E, Baltimore, Maryland 21231
Established in the 1990s, the Express Radiology Research Laboratory performs advanced radiology training and critical large animal research. The lab enables Johns Hopkins researchers to evolve benchside research into clinical trials for industry practice and improved patient health care. This stage of research includes feasibility studies, iterative testing and advanced training, all of which can provide a basis for subsequent design of clinical trials that will improve individualized patient health care. The lab has a large animal minimally-invasive suite, a Philips Allura C-Arm, is fully staffed and has the equipment needed to perform large animal preclinical studies. The lab is growing and will be adding additional equipment and expertise in time. All animal studies are approved by the Johns Hopkins Animal Care and Use Committee, ensuring compliance with medical ethics.
The Express Radiology Research Lab’s mission is to train, educate and conduct research, and it is uniquely qualified to perform advanced imaging studies. Team members are brought together to solve today’s medical mysteries by developing new imaging techniques, improving medical devices and communicating information with stunning clarity.
The Express Radiology Research Lab provides a training ground not only for radiologists but also for collaborations between engineers, physicists, doctorate holders, physicians, industry and other faculty specialists interested in perfecting advanced imaging techniques, in order that specialists can develop technology ultimately used to offer progressive personalized care for patients
The mission of the MRI Service Center is to explore and investigate all aspects of magnetic resonance research in medicine. The center serves as a research resource for members of the Department of Radiology, Johns Hopkins and the community. It invites and encourages research innovation, as well as collaboration at the local, national and international level, and works to provide state-of-the-art technology to researchers and clinicians.
Our three clinical human MRI scanners are dedicated for research and staffed by three MR technologists who assist with all imaging procedures.
- 3T Siemens TIM Prisma MRI provides multinuclear imaging capabilities and offers a full complement of receiver coils for neurological, musculoskeletal and body imaging. Equipped with 64 independent radio-frequency channels for parallel transmit and receive and high-performance gradients for high signal-to-noise ratio (SNR), speed and enhanced diffusion-weighted MRI (DWI). It has a dockable table to facilitate patient transfer and anesthesia, a video system for functional MRI (fMRI) studies and a 3D neuro-software package for DWI.
- 1.5T short-bore Siemens Espree MRI with a miyabi transfer table connecting a flat-panel, floor-mounted Siemens Axiom Artis Zee angiographic system for cardiac-gated, c-arm/cone beam CT. It is ideally suited for MRI-guided interventional studies and is equipped with a cyro-ablation system.
- 3T Philips Achieva MRI with in-room monitors to facilitate dynamic and interventional studies, 32 receive channels, a patient-adaptive two channel RF shimming, multinuclear MR capabilities (including phosphorus-31P) and a full complement of coils for neurological, musculoskeletal and body MRI. The system has Quasar dual gradients, with gradients up to 80 mT/m, slew rates up to 200 mT/ms, a projection system for fMRI studies, and a secondary table for MRI-guided high-intensity ultra-sound (HIFU) procedures with real-time temperature mapping.
- Work station software for developing and testing new MR pulse sequences.
- Vendor research agreements for work-in-progress sequences and multicenter trials.
Johns Hopkins University
Department of Radiology
600 N. Wolf Street, Park 311
Baltimore, MD 21287
Positron emission tomography (PET) is a highly sensitive, radionuclide-based molecular imaging modality for detecting and measuring receptors, transporters, enzymes, ion channels and other key biological mediators in relevant animal models and in human subjects.
Our mission is to lead in discovery, teaching and application of PET agents and methods for justly distributed and improved person-centric care.
The Johns Hopkins PET radiotracer center has been in continuous operation since installation of our first cyclotron in 1983. It is one of the premier centers for the discovery of new radiotracers, implementation of promising new radiotracer discovered elsewhere and for managing patients in our community.
PET is a molecular imaging technique that measures biochemistry noninvasively from preclinical studies in small animals to human beings. The technique works by detecting the annihilation of positrons that occur within living subjects after administration of a positron-emitting radiotracer that is targeted to a specific receptor, transporter, enzyme or antigen. The annihilation photons are detected as they arrive in coincidence at opposite detectors placed around the patient in the scanner. An image is reconstructed from the position of the detected photons as they leave the body creating an image of biochemical activity occurring at the diseae site and in the whole body. PET scanning has been a clinically valuable technique for both research and patient management since the 1980s. However, it is only been within the last 20 years that the Centers for Medicare & Medicaid Services (CMS) has reimbursed for clinical PET studies, making them widely available in the US.
Rarely are such advanced PET imaging studies performed alone. Currently, PET is performed in conjunction with computed tomography (CT) that provides anatomic information and the fused molecular and anatomic (CT) images are interpreted together as PET/CT studies. The majority of those studies utilize a positron-emitting form of glucose, known as fluorodeoxyglucose (FDG), to check for tumor metabolism. But there are many other such studies that we preform clinically to guide disease diagnosis and monitoring response to therapy, as detailed below:
- FDG PET/CT for Oncologic Imaging, using the metabolic biomarker [18F]FDG to help diagnose cancer and detect the spread of cancer to other organs, as well measure the effectiveness of anti-cancer therapy.
- Sodium Fluoride PET/CT for Bone Imaging, using the [18F]NaF, a highly sensitive bone-seeking PET tracer, to detect skeletal abnormalities.
- FDG PET/CT for Brain Imaging, used to evaluate for cognitive impairment, dementia and seizure disorders.
- AMYLOID PET/CT, using biomarkers such as [18F]florbetaben and [18F]florbetapir used to estimate amyloid plaque density in patients with cognitive impairment, Alzheimer’s disease and other causes of cognitive decline.
- Myocardial Viability Imaging, uses [18F]FDG PET/CT to assess myocardial viability in patients with advanced coronary artery disease and left ventricular dysfunction.
- Myocardial Perfusion Imaging, uses 13N-ammonia PET/CT for high-sensitivity detection of occlusive coronary artery disease, evaluation of the effectiveness of therapeutic interventions, and evaluation of quantitative myocardial flow reserve.
- Inflammation and Infection Imaging, uses [18F]FDG PET/CT for detection of sarcoidosis, large-vessel vasculitis, implant related infections, and fever of unknown origin.
- [68Ga]Dotatate PET/CT Imaging of somatostatin receptors for highly sensitive detection and treatment follow-up of neuroendocrine tumors.
- PET scan slot: $1,225
- HRRT brain BET slot: $2,025
- Residual [18F] flouride: $150
- Dedicated [18F] flouride: $500
The mission of the Center for Translational Molecular Imaging (CTMI), a section within the Division of Nuclear Medicine and Molecular Imaging at Johns Hopkins, is to serve as an Academic Research Organization (ARO) to develop and provide state-of-the-art imaging and therapeutic agents to address unmet medical needs to bring personalized medicine within the reach of patients. In the CTMI, we address a variety of disorders including cancer, psychiatric and neurodegenerative diseases, cardiovascular disease, among others. On-site human 3T MRI and positron emission computed tomography/computed tomography (PET/CT) are available.
The CTMI was established to answer challenges in the translation of promising new imaging agents and medicines, which despite their promise to deliver personalized medicine, have no ready commercial backing in the early stages of development. We are specifically designed to undertake initial phase 1 (first-in-human) trials, and address the challenges of moving new molecular imaging or companion therapeutic agents to the clinic. In collaboration with JHMI, we can provide MRI/PET/SPECT imaging services for all clinical trials.
CTMI leverages discoveries from investigators at Johns Hopkins, other academic institutions and industry, turning them into clinical applications as rapidly as possible through our nimble, and clinically focused operating procedures. CTMI is led by a team of highly-qualified, results-oriented individuals with key complementary skills for clinical translation of imaging agents and theranostics.
As an ARO, the CTMI actively solicits industrial collaborations and sponsorship to bring valuable new agents to clinical fruition in a timely manner. As a unique ARO, we cut across traditional silos by bridging academic and commercial missions.
Leveraging resources at Johns Hopkins University, the CTMI will generate new products through the offices of Technology Ventures and Business Development and Strategic Alliances.
Learn more about the CTMI at www.jhctmi.org.
The Center for Translational Molecular Imaging
G Building on the Johns Hopkins Bayview Medical Campus
4940 Eastern Avenue
Baltimore, Maryland 21224
Your generous support helps to fund the latest advancements in radiological technology. Your charitable gift helps us to innovate as we work toward new, more revealing perspectives on the human body, advance our understanding of disease, and discover new treatment methods that improve outcomes for patients at Johns Hopkins and around the world.
Established in 2004, the MRB Molecular Imaging Service Center and Cancer Functional Imaging Core provides comprehensive molecular and functional imaging infrastructure to support the imaging research needs of the Johns Hopkins University faculty. Approximately 55-65 different Principal Investigators use the center annually.
The MRB Molecular Imaging Service Center is located behind the barrier within the transgenic animal facility in the basement of MRB.
- 9.4T MRI/S scanner for magnetic resonance imaging and spectroscopy
- Olympus multiphoton microscope with in vivo imaging capability
- PET-CT scanner
- PET-SPECT scanner,
- SPECT-CT scanner for nuclear imaging
- Multiple optical imaging scanners including an IVIS Spectrum
- LI COR near infrared scanner
- Ultrasound scanner.
A brand new satellite facility in CRB2-LB03 houses a simultaneous 7T PET-MR scanner, as well as additional imaging equipment, to meet the growing molecular and functional imaging research needs of investigators.
MRB Animal Facility training session and Imaging Center Orientation session are required to obtain access to the MRB Animal Facility and to the MRB Molecular Imaging Center (Suite B14). Please keep in mind that obtaining access to both facilities requires time, so please plan in advance.
- The MRB Animal Facility training group meets at 9:30 am on Thursdays at the Turner fountain/MRB elevator lobby.
- The Imaging Center orientation group meets at 1 pm on Thursdays at the Turner fountain, and orientation takes approximately 30 min.
The service center has dedicated technical and administrative staff to assist users. Reservations and charging are done through iLab. New users should contact Dr. Elena Artemova (email@example.com) and Mr. Desmond Jacob (firstname.lastname@example.org).Miller Research Building of the Johns Hopkins University School of Medicine
733 North Broadway
Baltimore, MD 21205
Zaver M. Bhujwalla, M.Sc., Ph.D.
Desmond Jacob, Research Associate
Xiaoju Yang, Research Technologist
Elena Artemova, Budget Analyst
The goal of our laboratory is to develop new MR technologies to improve the resolution and contrast of MRI and apply them to observe brain anatomy to answer various types of biological questions.