Dr. Wade is interested in the emergence of structural genomics, proteomics and the large-scale sequencing of many genomes provides experimental access to regions of protein sequence-structure-function landscapes which have not been explored through traditional biochemical methods. Dr. Wade and his lab will use protein libraries, chemistry, biophysics, molecular biology and structural methods to examine the basis of molecular recognition in the context of several important biological problems, including structural and mechanistic aspects of multi-drug resistance, ligand-dependent molecular switches and metal ion homeostasis.
The Wade Lab would like to understand how biological systems read and transform chemical signals into a diverse range of biological outputs. To better understand the functional roles of ligand interactions in regulatory systems, the Wade laboratory focuses on BmrR and MDR functions that are stimulated by chemically diverse small-molecules. Biochemical, biophysical and x-ray crystallographic approaches are employed to dissect and examine ligand-directed effects at the binding, functional and structural levels. The aim is to combine functional, thermodynamic and structural studies into a more integrative analysis of ligand recognition and ligand-dependent control of protein conformation.
Lab Website: Herschel Wade Lab
L.A. Brammer, J.M. Smith, H. Wade, C. Freel Meyers. 2011. "1-Deoxy-D-xylulose 5-phosphate synthase: A new twist in thiamine diphosphate-dependent enzymology." J. Biol. Chem. in the press, doi:10.1074/jbc.M111.259747
S. Bachas, C. Eginton, D. Gunio, H.Wade. "Contributions to multidrug recognition in BmrR." PNAS 108(27) 11046-11051. 2011.
H. Wade. "MD recognition by MDR gene regulators Current Opinion in Structural Biology" 20(4): 489-496. 2010.
S. Hu, Z. Xie, A. Onishi, X. Yu, L. Jiang, J. Lin, H.S. Rho, C. Woodard, H. Wang, J.S. Jeong, S. Long, X. He, H. Wade, S. Blackshaw, J. Qian, and H. Zhu. "Profiling the human protein-DNA interactorne reveals ERK2 as a transcriptional repressor of interferon signaling." Cell 139(3): 610-622. 2009
H. Wade, S. Stayrook, W.F. DeGrado "Diferric structure of a model diiron protein; implications for cofactor stabilization and catalysis." Angew. Chem. Int. Ed. 45(30): 4951.4954. 2006.