Dr. Clarke's research interests primarily involve the development of analytical methods for drug analysis. This includes the development and implementation of tandem mass spectrometry and high resolution-accurate mass (HRAM) spectrometry method for measurement of multiple drugs in a single sample, as well as investigation of alternative matrices for drug testing. In addition, he is interested in the application of pharmacokinetic measurements, along with pharmacogenetic testing, to clinical management of patients.
Active projects in his lab include include qualitative screening for antiretroviral drugs and substances of abuse in various HIV- prevention clinical trials, development and validation of mass spectrometry methods for clinical analysis, and development of clinical assays for use on microfluidic platforms. He and his team are also investigating ways to improve the robustness and throughput of LC-MS methods in the clinical laboratory by the evaluation of newly developed technologies and alternative approaches to LC-MS method development.
Lab Website: Advanced Clinical Chemistry Diagnostics Laboratory
Technology Expertise Keywords
LC-MS/MS, chromatography, mass spectrometry, immunoassay
Olson MT, Breaud A, Harlan R, Emezienna N, Schools S, Yergey AL, and Clarke W. "Alternative Calibration Strategies for the Clinical Laboratory: Application to Nortriptyline Therapeutic Drug Monitoring, Clin Chem, 2013; 59 (6): 920-927.
Marzinke MA, Breaud A, Parsons TL, Cohen MS, Piwowar-Manning E, Eshleman SH, and Clarke W. "The Development and Validation of a Method Using High-Resolution Mass Spectrometry (HRMS) for the Qualitative Detection of Antiretroviral Agents in Human Blood", Clin Chim Acta, 2014; 433: 157-1681)
Clarke W. "What is Needed for Optimal Therapeutic Drug Monitoring", Bioanalysis, 2014; 6 (2): 113-115.
Marzinke MA, Petrides AK, Steele K, Schweitzer MA, Magnuson TH, Reinblatt SP, Coughlin JW, and Clarke W. "Decreased Escitalopram Concentrations Post Roux-en-Y Gastric Bypass Surgery", Ther Drug Monit, 2015; 37 (3): 408-412.
Olson MT, Baxi A, ElNaggar, M, Umbricht C, Yergey AL, and Clarke W. "Morphologically Compatible Mass Spectrometric Analysis of Lipids in Cytological Specimens", J Am Soc Cytopath, 2016; 5 (1): 3-8.