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Alexandra Valsamakis, M.D., Ph.D.

Photo of Dr. Alexandra Valsamakis, M.D., Ph.D.

Director, Clinical Virology and Molecular Microbiology

Professor of Pathology


Expertise: Pathology

Research Interests: Viral infections of immunocompromised patients; Hepatitis C and B infections; Respiratory virus infections


The Johns Hopkins Hospital

600 N. Wolfe Street
Department of Pathology
Baltimore, MD 21287 map


Dr. Alexandra Valsamakis is a professor of pathology at the Johns Hopkins University School of Medicine. She holds a joint secondary appointment in Molecular Microbiology and Immunology in the Johns Hopkins Bloomberg School of Public Health and serves as director of Clinical Virology and Molecular Microbiology Laboratories in the Department of Pathology at the Johns Hopkins Hospital.

She received an M.D. and Ph.D. (microbiology) from the University of Pennsylvania School of Medicine. After a residency in clinical pathology at the University of California-San Diego, she completed two fellowships at Johns Hopkins, one molecular virology and a second in medical microbiology.

Dr. Valsamakis’ research focuses on the detection, identification, and management of viral infections.

She is past-president of the Pan American Society for Clinical Virology and has served as chair of the Infectious Diseases Subdivision of the Association for Molecular Pathology. She has contributed to the field of molecular pathology in a number of different capacities including editorial board memberships (Diagnostic Microbiology and Infectious Diseases, Manual of Clinical Microbiology), ad hoc reviewer (Journal of Clinical Microbiology, Journal of Clinical Virology, Journal of Molecular Diagnostics, Clinical Diagnostic and Laboratory Immunology, Journal of Viral Hepatitis), committee member/co-author “Quantitative Methods for Infectious Diseases” (Clinical Laboratory Standards Institute), grant reviewer (member of IDM-B-12, an SBIR study section), meeting and course organizer (Association for Molecular Pathology Annual Meetings, 2006, 2007, 2015, 2016, and AMP/AACC course “Molecular Pathology Essentials: Principles and Practice”, 2008, 2010, 2012), participant in numerous industrial advisory boards, invited lecturer, and game show hostess. more


  • Director, Clinical Virology and Molecular Microbiology
  • Professor of Pathology

Departments / Divisions



  • MD, Hospital of the University of Pennsylvania - REGISTRAR (1992)


  • University of California / Pathology (1994)


  • Johns Hopkins Bloomberg School of Public Health / Microbiology and Immunology (1997)
  • Johns Hopkins University School of Medicine / Medical Microbiology (1998)

Board Certifications

  • American Board of Pathology / Clinical Pathology (1996)

Research & Publications

Research Summary

Dr. Valsamakis' research focuses on defining the performance characteristics of new virologic tests (self-designed or commercial), determining the clinical utility of virologic tests, and understanding virologic disease through the use of diagnostic tests.

Technology Expertise Keywords

Molecular Infectious Disease; Molecular Virology Testing

Selected Publications

Kanakry JA, Hegde AM, Durand CM, Greer AE, Ambinder RF, Valsamakis A. The clinical significance of Epstein-Barr virus DNA in the plasma and peripheral blood mononuclear cells of patients with and without Epstein-Barr virus-associated diseases. Blood. 2016;127(16)2007-17

Valsamakis A. Standardization of viral load testing for cytomegalovirus: the long road just got longer. Clin. Infect. Dis. 2016;63(5)590-3

Kuo IC, Espinosa C, Forman M, Valsamakis A. A polymerase chain reaction-based algorithm to detect and prevent transmission of adenoviral conjunctivitis in hospital employees. Amer J Opthalmol. 2016;163:38-44

Greer AE, Forman MS, Valsamakis A. Comparison of BKV quantification using a single automated nucleic acid extraction platform and three real-time PCR assays. Diagn Microbiol Infect Dis. 2015;82(4):297-302

Durand CM, Marr KA, Arnold C, Tang L, Durand DJ, Avery RK, Valsamakis A, Neofytos D. Detection of cytomegalovirus DNA in plasma as an adjunct diagnostic for gastrointestinal disease in kidney and liver transplant recipients. Clin Infect Dis. 2013;57(11):1550-59

Academic Affiliations & Courses

Graduate Program Affiliation

Graduate Program in Molecular Microbiology and Immunology

Activities & Honors


  • American Society for Microbiology
    President, Maryland Branch, 2002 - 2004
  • Association for Molecular Pathology
    Chair-Elect, Infectious Diseases Subdivision
  • Infectious Disease Society of America
  • Pan-American Society for Clinical Virology

Professional Activities

  • Ad hoc reviewer, Journal of Clinical Microbiology
  • Ad hoc reviewer, Journal of Molecular Diagnostics
  • Ad hoc reviewer, Diagnostic Microbiology and Infectious Diseases

Patient Ratings & Comments

The Patient Rating score is an average of all responses to physician related questions on the national CG-CAHPS Medical Practice patient experience survey through Press Ganey. Responses are measured on a scale of 1 to 5, with 5 being the best score. Comments are also gathered from our CG-CAHPS Medical Practice Survey through Press Ganey and displayed in their entirety. Patients are de-identified for confidentiality and patient privacy.

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