Dr. Ness has an extensive background in research activities related to blood safety. In past years, his laboratory has emphasized the development of assays for detecting red cell antibodies and small populations of heterogeneous red cells using a quantitative enzyme-linked antiglobulin test. This assay has proved useful in the study of fetal-maternal hemorrhage, red cells survival studies and autoimmune hemolytic anemia. The division has also studied the pathophysiology of delayed hemolytic transfusion reactions using a rabbit model and is undertaking studies of red cell alloimmunization in mice and rabbits.
Ongoing clinical studies in transfusion medicine include the use of hemodilution in elective surgery; assessment of the risk of viral and bacterial infections in blood recipients; research on the recurrence of cancer as related to the immunosuppressive effects of blood transfusions, the development and use of hemoglobin-based oxygen carriers as blood substitutes, and the use of pathogen inactivated platelets in Oncology and whole blood in Uganda.
The Transfusion Medicine Laboratory provides blood components and immunohematologic diagnostics for all of the patients at Johns Hopkins. The research goals of the laboratory derive from that large clinical experience, where the many complications of transfusion, including infectious complications and alloimmunization, are studied. The laboratory also aims to maximize transfusion benefits by studies of blood component manipulation and platelet crossmatching. Its studies on infectious complications have also focused on national and international collaborations in Thailand and China. The laboratory has been funded to be a core clinical center in the NHLBI Transfusion Medicine/Hemostasis Clinical Trial Network.
The division also has studied the immune response to transfusion extensively. In recent years, the studies have shifted to animal models of alloimmunization to red cells in rabbits and mice. These studies will be potentially important to limit the complications of hemolytic transfusion reactions to blood components or in stem cell graft recipients with chimeric red cell populations.
She L, Wang JX, Stevels L, Ness P, Shan H. "Blood safety and availability: continuing challenges in China’s blood banking system." Transfusion. 2014 Feb;54(2):471-82.
Savage W, Tobian AAR, Ness PM, Kaufman RM. "Desensitization in allergic transfusion reactions: Evidence from the trial to reduce alloimmunization to platelets." Transfusion. 2014;54(2):496-498.
Stonemetz JL, Allen PX, Wasey J, Rivers RJ, Ness PM, Frank SM. "Development of a risk-adjusted blood utilization metric." Transfusion. 2014.
Kakcer S, Ness PM, Savage WJ, Frick KD, Shirey S, King KE, Tobian AAR. "Economic evaluation of a hypothetical screening assay for alloimmunization risk among transfused patients with sickle cell disease." Transfusion. 2014.
Hervig T, Doughty H, Ness PM, Badloe JF, Berseus O, Glassberg E, Heier HE. "Prehospital use of plasma: The blood bankers’ perspective." Shock. 2014; 41 (SUPP. 1):70-75.
Steiner ME, Ness PM, Assman SF, Triulzi DJ, Sloan DR, Delaney M, Granger S, Bennett-Guerrero E, Blachman MA, Scavo V, Carson JL, Levy JH, Whitman G, D'Andra P, Pulkrabek S, Ortel TL, Bornikova L, Raife T, Puca KE, Kaufman RM, Nuttall GA, Young PP, Youssef S, Engelman R, Greilich PE, Miles R, Josephson CD, Bracey A, Cooke R, McCullough, J, Hunsaker R, Uhl L, McFarland JG, Park Y, Cushing MM, Klodell CT, Karanam R, Roberts PR, Dyke C, Hod EA, Stowell CP. Effects of red-cell storage duration on patients undergoing cardiac surgery. N Engl J Med 372:1419-29, 2015.
Frank, S. M., Rothschild, J. A., Masear, C. G., Rivers, R. J., Merritt, W. T., Savage, W. J., & Ness, P. M. Optimizing preoperative blood ordering with data acquired from an anesthesia information management system. , Anesthesiology 118(6), 1286-1297, 2013
Anderson KC, Ness PM. Scientific basis of transfusion medicine: Implications for clinical practice. Second Edition, WB Saunders, Philadelphia PA 1999.
Savage WJ, Tobian AAR, Savage JH, Hamilton RG, Ness PM. Atopic predisposition of recipients in allergic transfusion reactions to apheresis platelets. Transfusion 51; 2337-2342, 2011.