Dr. Inoue and his lab have developed a series of chemical-molecular tools that allow for inducible, quick-onset and specific perturbation of various signaling molecules. Using this novel technique in conjunction with fluorescence imaging, microfabricated devices, quantitative analysis and computational modeling, the Inoue Lab is dissecting intricate signaling networks. They investigate positive-feedback mechanisms underlying the initiation of neutrophil chemotaxis (known as a symmetry breaking process), as well as spatio-temporally compartmentalized Ras signaling. In parallel, the lab also tries to understand how cell morphology affects biochemical functions in cells. Ultimately, the team’s research seeks to generate completely orthogonal nano-machinery in cells that can achieve existing, as well as novel, cellular functions.
Synthetic cell biology: total synthesis of cellular functions such as neutrophil chemotaxis and ciliary mechano-sensation
Our research focuses on "synthetic cell biology" to dissect and reconstitute intricate signaling networks. In particular, we investigate positive-feedback mechanisms underlying the initiation of neutrophil chemotaxis (known as a symmetry breaking process), as well as spatio-temporally dynamic information processing at various compartments in living cells. In parallel, our lab also tries to understand how cell morphology affects biochemical functions. Ultimately, we will generate completely orthogonal nano-machinery in artificial cells that can achieve existing, and even novel, cellular functions.
Our research is conducted in an open lab style building that embodies a multidisciplinary research approach. Students who are enthusiastic about learning diverse disciplines, developing innovative techniques and challenging fundamental biological problems would enjoy the environment. Students are always welcomed to our lab for discussions about potential research projects.
Lab Website: Inoue Lab
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Deb Roy A, Gross EG, Pillai GS, Seetharaman S, Etienne-Manneville S, Inoue T Non-catalytic allostery in α-TAT1 by a phospho-switch drives dynamic microtubule acetylation Journal of Cell Biology 2022;221:11:e1-20
Nihongaki Y, Matsubayashi HT, Inoue T. A molecular trap inside microtubules probes luminal access by soluble proteins Nature Chemical Biology 2021;17:888-895
Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, Umehara T, Inoue T Rational design and implementation of a chemically inducible hetero-trimerization system Nature Methods 2020;17:928-936
Nakamura H, Lee AA, Afshar AS, Watanabe S, Rho E, Razavi S, Suarez A, Lin YC, Tanigawa M, Huang B, DeRose R, Bobb D, Hong W, Gabelli SB, Goutsias J, Inoue T Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions Nature Materials 2018;17:79-89
Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Schurmans S, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. Cell 2017;168:264-279