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L. Mario Amzel, Ph.D.

Headshot of L. Mario Amzel
  • Director, Department of Biophysics and Biophysical Chemistry
  • Professor of Biophysics and Biophysical Chemistry

Research Interests

Nudix hydrolases; PI3K; VP14; Structural enzymology of redox and phosphoryl-transfer enzymes; Selected areas of structural thermodynamics; MICAL; Redox and phosphoryl transfer enzymes; Three dimensional structure and mechanism of proteins ...read more

Background

Dr. L. Mario Amzel is professor of biophysics and biophysical chemistry at the Johns Hopkins University School of Medicine. Dr. Amzel serves as the director of the Department of Biophysics and Biophysical Chemistry.

His research focuses on structural mechanistic biochemistry and structural thermodynamics. Amzel was the first to uncover the structure of part of an antibody, the molecule that helps the human immune system fight off infection. He was also part of a team of researchers at Johns Hopkins who produced the first high-resolution pictures of how antibodies interact with antigens.

Dr. Amzel’s research focuses on understanding protein structure and function, especially as is relevant to the immune system, infectious disease and cancer. His research team studies the chemistry of reactions carried out by enzymes. By determining the three-dimensional structures of these proteins and the conditions that affect their efficiency, they are able to figure out the roles they play in the body. Some of the information they gather is being used to design drugs targeting the enzymes.

Dr. Amzel earned his Ph.D. from the Universidad de Beunos Aires in Argentina in 1968. He joined Johns Hopkins in 1969 for a postdoctoral fellowship and stayed, earning full professorial status in 1984.

Amzel is an active lecturer at Johns Hopkins where he serves as course director of “Biochemical and Biophysical Principles.” He was awarded the 1994 Teacher of the Year Award and, in 1999, he received the University Alumni Teaching Award. In 2013, Amzel was admitted as a fellow into the American Association for Advancement of Science. He serves on numerous committees at Johns Hopkins and is on the editorial boards of several leading scientific publications.

...read more

Titles

  • Director, Department of Biophysics and Biophysical Chemistry
  • Professor of Biophysics and Biophysical Chemistry
  • Professor of Oncology

Departments / Divisions

Centers & Institutes

Research & Publications

Research Summary

Dr. Amzel’s research focuses on structural mechanistic biochemistry and structural thermodynamics. Amzel was the first to uncover the structure of part of an antibody, the molecule that helps the human immune system fight off infection. He was also part of a team of researchers at Johns Hopkins who produced the first high-resolution pictures of how antibodies interact with antigens, foreign molecules invading the body.

Since then, Amzel has determined the structure of many proteins and protein complexes. Amzel also studies how proteins fold and take on their three-dimensional shapes and how proteins catalyze biochemical reactions within cells. Some of his findings are being used to design drugs targeting the enzymes.

Lab

Dr. Amzel’s lab is studying the catalytic mechanism of several enzyme families, using molecular biology, biochemistry and structural biology. They are studying both enzymes that recognize or process phosphates and redox enzymes. Cloning and expression, ultrapurification, kinetic characterization, mutational analysis, mass spectrometry, crystallization, and structure determination by x-ray diffraction are some of the techniques the lab uses to characterize the mechanisms of these enzymes. As the team uncovers information about the enzyme systems, other researchers are designing new drugs based on their discoveries.

In addition to enzyme studies, Dr. Amzel’s lab studies structural thermodynamics. Researchers in his lab have developed several systems, including anti-peptide antibodies and lectins, to see how proteins interact and bond to macromolecules or ligands. They are developing computational methods to calculate the changes in the thermodynamic variables that take place when a protein recognizes another macromolecule or a small ligand. As research in this lab reveals more about binding energetics, other researchers can take that knowledge and apply it to structure-based drug design.

Lab Website: Structural Enzymology and Thermodynamics Group

Selected Publications

View all on PubMed

Nathan S, Gabelli SB, Yoder JB, Srinivasan L, Aldrich RW, Tomaselli GF, Ben-Johny M, Amzel, L.M..  (2021) Structural basis of cytoplasmic NaV1.5 and NaV1.4 regulation. J Gen Physiol. 153(1):e202012722. doi: 10.1085/jgp.202012722. PMID: 33306788

Llorente-Esteban A, Manville RW, Reyna-Neyra A, Abbott GW, Amzel, L.M., Carrasco N. (2020) Allosteric regulation of mammalian Na+/I- symporter activity by perchlorate. Nat Struct Mol Biol. 27(6):533-539. doi: 10.1038/s41594-020-0417-5. Epub PMID: 32451489

Chakrabarti M, Gabelli SB, Amzel, L.M.  (2020) Allosteric Activation of PI3Kα Results in Dynamic Access to Catalytically Competent Conformations. Structure pii: S0969-2126(20)30010-1. doi: 10.1016/j.str.2020.01.010. [Epub ahead of print] PMID: 32049032 [PubMed - as supplied by publisher]

Yoder JB, Ben-Johny M, Farinelli F, Srinivasan L, Shoemaker SR, Tomaselli GF, Gabelli SB, Amzel, L.M..(2019) Ca(2+)-dependent regulation of sodium channels Na(V)1.4 andNa(V)1.5 is controlled by the post-IQ motif. Nat Commun. Apr 3;10(1):1514. doi: 10.1038/s41467-019-09570-7. PubMed PMID: 30944319; PubMed Central PMCID: PMC6447637

Maheshwari S, Shimokawa C, Rudzka K, Kline CD, Eipper BA, Mains RE, Gabelli SB, Blackburn N and Amzel, L.M..  (2018) Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydoxylating monooxygenase. Communications Biology 1:74. doi: 10.1038/s42003-018-0082-y. eCollection 2018. PMID: 30271955 [PubMed]

Academic Affiliations & Courses

Graduate Program Affiliation

BCMB Program

Intercampus Program in Molecular Biophysics

Pharmacology and Molecular Sciences

Courses and Syllabi

  • Biochemical and Biophysical Principles

Activities & Honors

Honors

  • Fellow, American Association for the Advancement of Science, 2013
  • University Alumni Teaching Award, 1999
  • Teacher of the Year Award, 1994

Professional Activities

  • Academic Council, Johns Hopkins University School of Medicine
  • Board of Directors, Johns Hopkins University School of Medicine, 2012
  • Course director, Biochemical and Biophysical Principles
  • President, Society of Latin American Biophysicists, 2006
  • Professorial Promotions Committee, Johns Hopkins University School of Medicine
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