Jonathan Carl Dudley, M.D.

Headshot of Jonathan Carl Dudley
  • Assistant Professor of Pathology

Research Interests

Molecular Genetic Pathology


Dr. Jonathan Dudley is an Assistant Professor of Pathology, Oncology, and Urology at the Johns Hopkins University School of Medicine, as well as the Director of Research and Development for the Johns Hopkins Molecular Diagnostics lab.

He completed his residency and fellowship training at the Massachusetts General Hospital and Stanford Hospital. His research focuses on the development of novel molecular diagnostic methods for the detection and classification of cancer from cytopathology specimens. His work has been recognized by the Young Investigator Award from the Association for Molecular Pathology and the Benjamin Castleman Award from the United States and Canadian Academy of Pathology. He is currently a member of the Johns Hopkins Ludwig Center and supported by the Damon Runyon Physician-Scientist Training Award. more


  • Assistant Professor of Pathology
  • Assistant Professor of Oncology
  • Assistant Professor of Urology

Departments / Divisions



  • MD; Johns Hopkins University School of Medicine (2013)


  • Anatomic Pathology; Massachusetts General Hospital (2015)
  • Anatomic Pathology; Stanford University School of Medicine (2016)


  • Molecular Genetic Pathology; Stanford University School of Medicine (2017)

Board Certifications

  • American Board of Pathology (Anatomic Pathology) (2016)
  • American Board of Pathology (Molecular Genetic Pathology) (2017)

Research & Publications

Technology Expertise Keywords

Molecular Genetic Pathology

Selected Publications

Dudley, JC, Diehn, M. Detection and diagnostic utilization of cellular and cell-free tumor DNA. Annual Review of Pathology: Mechanisms of Disease, 16: 2021. 

Cohen, JD, Douville, C, Dudley, JC, Mog, BJ, Popoli, M, Ptak, J, Dobbyn, L, Silliman, N, Schaefer, J, Tie, J, Gibbs, P, Tomasetti, C, Papadopoulos, N, Kinzler, K, Vogelstein, B. Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands. Nature Biotechnology, 2021

Dudley, JC, Schroers-Martin, JG, Lazzareschi, DV, Shi, WY, Chen, S, Esfahani, MS, Trivedi, D, Chabon, JJ, Chaudhuri, AA, Stehr, H, Lim, H, Costa H, Nabet, BY, Sin, MLY, Liao, JC, Alizadeh, AA, Diehn, M. Detection and surveillance of bladder cancer using urine tumor DNA. Cancer Discovery, 9(4): 2019.

Dudley, JC, Lin, MT, Le, DT, Eshleman, JR. Microsatellite instability as a biomarker for PD-1 blockade. Clinical Cancer Research, 22(4): 2016

Dudley, JC, Zheng, Z, Le, LP, Dias-Santagata, D, Borger, D, Batten, J, Vernovsky, K, Sweeney, B, Arpin, R, Brugge, WR, Forcione, DG, Pitman, MB, Iafrate, AJ. Next-generation sequencing and fluorescence in situ hybridization have comparable performance characteristics in the analysis of pancreaticobiliary brushings for malignancy. Journal of Molecular Diagnostics, 18(1): 2016

Contact for Research Inquiries

Email me

Activities & Honors


  • K08 Award, NCI/NIH, 2023 - 2028
  • Damon Runyon Physician Scientist Training Award, Damon Runyon Cancer Research Foundation, 2020 - 2024
  • Benjamin Castleman Award, United States and Canadian Academy of Pathology, 2020
  • Pathologist Power List, The Pathologist Magazine, 2020
  • Young Investigator Award, Association for Molecular Pathology, 2018

Patient Ratings & Comments

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