H. Benjamin Larman, Ph.D.

Headshot of H. Benjamin Larman
  • Associate Professor of Pathology


Dr. Larman trained in physics and bioengineering at UC Berkeley, and then in genetics and materials science at the Harvard-MIT graduate program in health sciences and technology. Here he developed Phage ImmunoPrecipitation Sequencing in the lab of Steve Elledge. He then did his postdoc at Scripps in the laboratory of Pete Schultz, where he combined multiplexed molecular assays with liquid handling automation. Dr. Larman joined the faculty at Johns Hopkins in 2014, where he is currently an Associate Professor in the Immunology Division of the Pathology Department. His laboratory develops and deploys novel molecular assays to advance our understanding of targeted immune responses. Dr. Larman trains students and postdocs from chemical, biological and engineering backgrounds to develop new techniques and analytical approaches. Alongside his academic career, Dr. Larman also works to translate technological innovations through company formation. 

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  • Associate Professor of Pathology

Departments / Divisions

  • Pathology - Immunopathology



  • Ph.D.; Massachusetts Institute of Technology (Massachusetts) (2012)
  • B.S.; University of California (Berkeley) (California) (2002)

Research & Publications

Research Summary

Bacteriophage display of synthetic peptide libraries for comprehensive, quantitative profiling of antibodies (PhIP-Seq) and proteases (SEPARATE); display of protein libraries for antigen discovery, protein-protein interaction studies, and drug target identification (MIPSA); highly efficient immune receptor repertoire analyses (FR3AK-seq); and ultrasensitive, multiplex RNA quantification techniques to monitor gene expression and detect microbes (LISH-seq, cRASL-seq).


The Larman Laboratory is part of the Immunology Division in the Pathology Department at the Johns Hopkins University School of Medicine, in Baltimore, Maryland, USA. The lab is situated within the Institute for Cell Engineering‘s Immunobiology Program. Our efforts are devoted to developing and deploying highly multiplexed techniques that can be used to better understand human immune responses. Much of our work combines DNA synthesis, high throughput DNA sequencing, and sample handling automation for massively parallel molecular measurements.

Lab Website: Larman Laboratory

Technology Expertise Keywords

Molecular biology, immunology, antibody profiling, immune repertoire analyses

Selected Publications

Credle JJ, Gunn J, Sangkhapreecha P, Monaco DR, Zheng XA, Tsai HJ, Wilbon A, Morgenlander WR, Rastegar A, Dong Y, Jayaraman S, Tosi L, Parekkadan B, Baer AN, Roederer M, Bloch EM, Tobian AAR, Zyskind I, Silverberg JI, Rosenberg AZ, Cox AL, Lloyd T, Mammen AL, Larman HB. Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries. Nat Biomed Eng. 2022 Aug;6(8):992-1003. doi: 10.1038/s41551-022-00925-y. PMID: 35986181

Monaco DR, Kottapalli SV, Breitwieser FP, Anderson DE, Wijaya L, Tan K, Chia WN, Kammers K, Caturegli P, Waugh K, Roederer M, Petri M, Goldman DW, Rewers M, Wang LF, Larman HB. Deconvoluting virome-wide antibody epitope reactivity profiles. EBioMedicine. 2022 Jan; 75:103747. doi: 10.1016/j.ebiom.2021.103747. PMID: 34922324; PMCID: PMC8688874

Venkataraman T, Valencia C, Mangino M, Morgenlander W, Clipman SJ, Liechti T, Valencia A, Christofidou P, Spector T, Roederer M, Duggal P, Larman HB. Analysis of antibody binding specificities in twin and SNP-genotyped cohorts reveals that antiviral antibody epitope selection is a heritable trait. Immunity. 2022 Jan 11;55(1):174-184.e5. doi: 10.1016/j.immuni.2021.12.004. PMID: 35021055.

Morgenlander WR, Henson SN, Monaco DR, Chen A, Littlefield K, Bloch EM, Fujimura E, Ruczinski I, Crowley AR, Natarajan H, Butler SE, Weiner JA, Li MZ, Bonny TS, Benner SE, Balagopal A, Sullivan D,  Shoham S, Quinn TC, Eshleman SH, Casadevall A, Redd AD, Laeyendecker O, Ackerman ME, Pekosz A, Elledge SJ, Robinson M, Tobian AAR, Larman HB. Antibody responses to endemic coronaviruses modulate COVID-19 convalescent plasma functionality. J Clin Invest. 2021;1;131(7):e146927. PMID: 33571169

Larman HB*, Salajegheh M*, Nazareno R, Lam T, Sauld J, Steen H, Kong SW, Pinkus JL, Amato AA, Elledge SJ, Greenberg SA. Cytosolic 5′-nucleotidase 1a autoimmunity in sporadic inclusion body myositis. Ann Neurol. 2013;73(3):408-418. PMID: 23596012; equal contribution.

Academic Affiliations & Courses

Graduate Program Affiliation


Chemical & Biomolecular Engineering

Biomedical Engineering


Videos & Media

Recent News Articles and Media Coverage

Phage ImmunoPrecipitation Sequencing (PhIP-seq), Harry Benjamin Larman YouTube (July 5, 2017)

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