Research Summary
Bacteriophage display of synthetic peptide libraries for comprehensive, quantitative profiling of antibodies (PhIP-Seq) and proteases (SEPARATE); display of protein libraries for antigen discovery, protein-protein interaction studies, and drug target identification (MIPSA); highly efficient immune receptor repertoire analyses (FR3AK-seq); and ultrasensitive, multiplex RNA quantification techniques to monitor gene expression and detect microbes (LISH-seq, cRASL-seq).
Lab
The Larman Laboratory is part of the Immunology Division in the Pathology Department at the Johns Hopkins University School of Medicine, in Baltimore, Maryland, USA. The lab is situated within the Institute for Cell Engineering‘s Immunobiology Program. Our efforts are devoted to developing and deploying highly multiplexed techniques that can be used to better understand human immune responses. Much of our work combines DNA synthesis, high throughput DNA sequencing, and sample handling automation for massively parallel molecular measurements.
Lab Website: Larman Laboratory
Technology Expertise Keywords
Molecular biology, immunology, antibody profiling, immune repertoire analyses
Selected Publications
Credle JJ, Gunn J, Sangkhapreecha P, Monaco DR, Zheng XA, Tsai HJ, Wilbon A, Morgenlander WR, Rastegar A, Dong Y, Jayaraman S, Tosi L, Parekkadan B, Baer AN, Roederer M, Bloch EM, Tobian AAR, Zyskind I, Silverberg JI, Rosenberg AZ, Cox AL, Lloyd T, Mammen AL, Larman HB. Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries. Nat Biomed Eng. 2022 Aug;6(8):992-1003. doi: 10.1038/s41551-022-00925-y. PMID: 35986181
Monaco DR, Kottapalli SV, Breitwieser FP, Anderson DE, Wijaya L, Tan K, Chia WN, Kammers K, Caturegli P, Waugh K, Roederer M, Petri M, Goldman DW, Rewers M, Wang LF, Larman HB. Deconvoluting virome-wide antibody epitope reactivity profiles. EBioMedicine. 2022 Jan; 75:103747. doi: 10.1016/j.ebiom.2021.103747. PMID: 34922324; PMCID: PMC8688874
Venkataraman T, Valencia C, Mangino M, Morgenlander W, Clipman SJ, Liechti T, Valencia A, Christofidou P, Spector T, Roederer M, Duggal P, Larman HB. Analysis of antibody binding specificities in twin and SNP-genotyped cohorts reveals that antiviral antibody epitope selection is a heritable trait. Immunity. 2022 Jan 11;55(1):174-184.e5. doi: 10.1016/j.immuni.2021.12.004. PMID: 35021055.
Morgenlander WR, Henson SN, Monaco DR, Chen A, Littlefield K, Bloch EM, Fujimura E, Ruczinski I, Crowley AR, Natarajan H, Butler SE, Weiner JA, Li MZ, Bonny TS, Benner SE, Balagopal A, Sullivan D, Shoham S, Quinn TC, Eshleman SH, Casadevall A, Redd AD, Laeyendecker O, Ackerman ME, Pekosz A, Elledge SJ, Robinson M, Tobian AAR, Larman HB. Antibody responses to endemic coronaviruses modulate COVID-19 convalescent plasma functionality. J Clin Invest. 2021;1;131(7):e146927. PMID: 33571169
Larman HB*, Salajegheh M*, Nazareno R, Lam T, Sauld J, Steen H, Kong SW, Pinkus JL, Amato AA, Elledge SJ, Greenberg SA. Cytosolic 5′-nucleotidase 1a autoimmunity in sporadic inclusion body myositis. Ann Neurol. 2013;73(3):408-418. PMID: 23596012; equal contribution.
