Research Summary
Dr. Raben is interested in the chemistry and biochemistry of lipids and lipid enzymes involved in signaling cascades. His laboratory’s major effort is studying the molecular aspects involved in regulating lipid metabolizing signaling enzymes and the physiological roles of this regulation. The control of these enzymes involves the modulation of two key parameters, their sub-cellular distribution and intrinsic enzymatic activity. The importance of this work is underscored by the fact that defects in lipid cascades often contribute to conditions such as diabetes, cardiovascular disease, neurological disorders, immune dysfunctions, and cancer.
Lab
Lab Website: Daniel Raben Laboratory
Selected Publications
Petro, E., and Raben, D.M. “Bacterial expression strategies for several Sus scrofa diacylglycerol kinase alpha constructs: solubility challenges.” Scientific Reports. 2013;3:160.
Ueda S., Tu-Sekine, B. Yamanoue, M., Raben, D.M., and Shirai, Y. “The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos.” BMC Developmental Biology. 2013. 13:35.
Bolduc, D., Rahdar, M., Tu-Sekine, B., Sivakumaren, S.C., Raben, D., Amzel, L.M., Devreotes, P, Gabelli, S.B., and Cole, P. “Phosphorylation-mediated PTEN conformational closure and deactivation revealed with protein semisynthesis.” Elife 2013 Jul 9;2:e00691.
Tu-Sekine, B., Goldschmidt, H., Petro, E, and Raben, D.M. “Diacylglycerol Kinase Theta: Regulation and Stability.” Adv. Biol. Reg. 2013. Jan;53(1):118-26.
Tu-Sekine B, and Raben, D.M. “Dual Regulation of DGK-θ: Polybasic Proteins Promote Activation by Phospholipids and Increase Substrate Affinity.” J. Biol. Chem. 2012. 287(50):41619-41627.
