Help transform how cancer research works. Help with us with this novel trial.
- Patients volunteer. Your patient sends us her tumor sequencing, and gives us permission to review her case.
- We identify eligible patients. We are looking for patients whose tumors display a spliceosome mutation which may produce more immunogenic antigens and thus be vulnerable to immune therapy.
- We contact you. We reach out to your office for details on her case, and provide kits for a blood sample
- Hopkins provides recommendations. Our molecular tumor board is a group of experts who help our oncologists interpret the complex data on sequencing reports. They will review your patient’s case and provide an opinion.
- You and your patient decide on therapy. If you pursue a recommended option, our report may help with insurance coverage, but you’re not obliged to follow our advice — you know your patient best.
- You provide data. We send a very brief survey periodically to see how your patient is doing. We then ask for feedback from you about your experience with the study.
PRISMM merges ideas from other trials you may already be familiar with: TAPUR, MATCH and the Metastatic Breast Cancer Project (MBCP). Like TAPUR and MATCH, we aim to measure how tumors with a specific mutation respond to a targeted medication. Like MBCP, we are reaching out through social media to identify patients and expand the pool of clinical trial participants into the community.
Community physicians face challenges in treating patients as cancer progresses and options become limited. Getting insurance to cover off-label medication use can be frustrating, even when a patient has a mutation that has responded in other tumor types. Newer drugs are offered only through clinical trials, which require living near a trial site. And for those patients who do have access to tertiary cancer centers, enrollment can disrupt the important longitudinal relationship with a trusted local oncologist.
This means most patients with advanced cancers seek treatment in the community, so valuable information about tumor, therapy and response is lost to science. This is particularly troubling for rare mutations, because an adequate population can’t be found for study.With its online recruitment strategy and observational structure, PRISMM aims to circumvent some of these problems.
Spliceosome mutations occur in a variety of cancers. The spliceosome is an intracellular machine that cuts processes (or splices) pre-messenger ribonucleic acids (pre-mRNA) to mature messenger RNA (mRNA). A single gene can be spliced in various ways, leading to variable protein activity.
Genetic mutations in the spliceosome cluster in “hotspot” regions. The most common involves SF3B1, which encodes a protein that appears to be important in correct spliceosome function. These mutations are found fairly commonly in MDS and CLL [1, 2]; they have also been observed less commonly in solid tumors, e.g., 19% in uveal melanoma, and 4% in breast and pancreatic cancers .
We and others have shown that creating an SF3B1 mutation in a cell like results in new mRNA transcripts, and that these transcripts are actually translated into abnormal proteins. [7, 8].
These preliminary data suggest that spliceosome mutations may produce a high number of neoantigens, or new proteins recognizable by the immune system. If true, this carries direct therapeutic consequences. In a landmark Hopkins’ study, Le et al. showed that colon cancers with a high mutational burden demonstrated dramatic responses to anti-PD1 therapy . This has led to an understanding that immune therapy is most effective when some kind of mutational burden produces high numbers of neoantigens.For this reason, we believe patients with tumors that harbor spliceosome mutations may respond to immune therapy, even if their tumor type or genetic analysis otherwise would not suggest this. We have some evidence that this may prove true: In an analysis of five immunotherapy trials that included gene sequencing, we identified 4/251 patients with spliceosome mutations, all of whom demonstrated at least a partial response to anti-PD1 therapy, one showing a durable remission. We also encountered through our molecular tumor board a patient with a long-term response to anti-PD1 therapy, whose tumor harbored a spliceosome mutation and did not display a high mutational burden.
Once we establish that your patient indeed has the mutation of interest, we will reach out to you to request your participation.
We will also request that you provide limited patient records (most recent clinic note describing the patient’s therapeutic history, most recent radiology and lab report), as well as a blood sample. The blood will be used to look for the mutational profile in the plasma (so-called plasma tumor DNA); the results will be used for investigational purposes only, and will not be considered by the tumor board. We will provide a kit for the blood draw and packaging to send it back to us at no charge within a week; no refrigeration or rush orders are necessary. We will ask that blood samples be sent to us, if possible, whenever you routinely draw blood on your patient; we will coordinate with your office to facilitate this.
The Hopkins molecular tumor board will review your patient’s case and provide recommendations. If the board does recommend immune therapy, you can use this documentation to support a request for insurance coverage or even compassionate use directly from the pharmaceutical company. We can provide guidance and forms for this process.
Once you receive the tumor board’s recommendations, you are under no obligation to follow them, of course. Regardless of the treatment path you and your patient choose, we hope that you will continue to participate in the study by sending us updates. We will send you very brief (1-2 minute) surveys inquiring about how your patient is doing. We will also provide you regular updates about how the trial is progressing.
When your involvement in the study ends, we will send a survey about your experience with the trial. In the interim, we are always open to suggestions from participating oncologists regarding improvements.