In This Section      

TCNP Protocols

BOEKE Lab Protocols

  • Yeast high efficiency transformation - dSLAM (PDF)

PANDEY Lab Protocols

  • Stable isotope labeling with amino acids in cell culture (SILAC) protocol for quantitation of protein dynamics (PDF)
  • 18O - labeling trypsin digest protocol for relative quantitation by mass spectrometry

COTTER Lab Protocols

  • Quantification of lysine acetylation (PDF)
  • N-terminal derivatization for de novo sequencing (PDF)
  • Determination of ubiquitylation sites (PDF)
  • Determination of SUMOylation sites (PDF)

COLE Lab Protocols

  • Chemical synthesis of a novel coenzyme A-based affinity probe (PDF)

MELUH Protocols

  • Indirect Immunofluorescence for Yeast (PDF)
  • DAPI Staining Yeast (PDF)
  • Chromatin Immunoprecipitation (ChIP) for Yeast (PDF)

RANDO Protocols