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High Throughput Biology – HIT Center

Test tubes in rack

The High Throughput Biology Center, or HiT Center, of the Institute for Basic Biomedical Sciences is an interdisciplinary and interdepartmental effort led by Joel S. Bader, Ph.D., Heng Zhu, Ph.D. and Seth Blackshaw, Ph.D. The three independent research labs that make up the HiT Center push the frontiers of research technologies and apply those techniques to fundamental questions of biology. The faculty members in the HiT Center combine approaches from a variety of disciplines including biology, physics, chemistry, mathematics, computer science and engineering. 

Goal

To accelerate hypothesis-driven research and to speed development of new hypotheses by selectively using high-throughput techniques.

Services

In addition to its active research programs, the HiT Center's core components offer resources to study interactions of chemical compounds, gene products, cells and organisms and the networks formed by these interactions. The Deep Sequencing & Microarray Core offers state-of-the art sequencing and array hybridization and analysis services. ChemCore is a compound screening and robotics service with access to compound libraries, and skill in assay development.

The HiT Center also delivers gene-specific reagents to Hopkins researchers though Genomics Resources, which provides automated picking of bacteria-containing plasmids, lentilviral-ready shRNA clones, and yeast deletion (YKO) strains. The core services are located on the third floor of the Edward D. Miller Research Building. Besides standard offerings, scientific collaborations with HiT Center faculty have led to innovative technological solutions to research problems.

  • Proteomics Core - Visit the site
    • The proteomics core provides extensive mass spectrometry services for analyzing protein mixtures and purified proteins. Protein profiling using two-dimensional gel analysis and fluorescent labeling is also offered.  The facility is directed by Robert Cole, Ph.D.
  • ChemCORE - Visit the site
    • ChemCore offers a structurally diverse compound repository and high-throughput robotics laboratory services. ChemCore scientists will work with clients to help them optimize their enzymatic and biologic assays in both 96 and 384 well formats. A diverse suite of assay options has been developed. For service inquiries and scheduling, please contact Alan Long, or submit your request online here
  • Deep Sequencing Microarray Core - Visit the site
    • The Deep Sequencing and Microarray Core provides cost-effective and time-efficient access to cutting-edge genomic technologies and expert assistance with experimental design and data analysis. Services include NextGen and Third Gen Sequencing; multiplatform microarray services including Affymetrix and Agilent; Nanostring nCounter Analysis services for multiplexed gene expression, miRNA expression, and copy number variation analysis; and DNA, Chromatin, and RNA shearing services. The core also provides statistical and bioinformatic data analysis on primary data and downstream functional analyses.  For more information please contact Haiping Hao, Ph.D.
  • Genomics Resources - Visit the site
    • Resources distribution site provides automated picking of resources like bacteria-containing plasmids or systematic yeast strain collections for Hopkins researchers. These resources can be picked to from collections of mammalian Open Reading Frames (ORFs), lentiviral-ready shRNA clones, and a wide variety of yeast deletion (YKO) strains. For more information, please contact Alan S. Long
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