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School of Medicine
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Genome Editing and Mouse Models
Roger Reeves, Ph.D.
Gene Editing Expertise Using CRISPR/CAS9
Advice and expertise in guide RNA design and selection for gene editing based on CRISPR/CAS9
Plasmid constructs for different components of CRISPR/CAS9 methodology
Advice and expertise in generating mouse models using CRISP/CAS9 technology
Gene editing of mouse oocytes
Conventional Transgenic Mouse Generation
Advice and expertise on construct design, insertion of spacers, enhancers and transcript stabilizing elements
Advice in screening transgenic mice (PCR, Southern blot)
Injection of construct into fertilized oocytes and implantation into pseudopregnant females
Conventional Knockout, Knock-In Mouse Generation
Advice and expertise in generating targeting constructs, screening methods/protocols and selection of loxP insertion sites
Culture and handling of ES cells
Advice and expertise in screening positive ES cell clones
Karyotype -ploidy analysis of ES cell clones
Cryopereservation of Sperm, Oocytes and Embryos
In conjunction with services offered by The Jackson Laboratory (Jax.org). Maintain active colony numbers at the appropriate size without keeping extra mice “on the shelf.” Provide convenient method for sharing or recovering mice from other institutions. Derive pathogen-free strains.