Zeiss LSM 510 Meta Confocal Microscope

Zeiss LSM 510 Meta Confocal and Zeiss LSM 510 Meta Offline Workstation

Z sectioning and 3D reconstruction.

The properties of the confocal laser scanning microscope allow for optical sectioning in the Z axis.  The pinhole, a small, adjustable diaphragm placed in front of the PMT, eliminates unwanted light from out-of-focus areas.  Capturing a series, or stack, of images focused at regularly placed intervals through the depth of an object of interest allows one to subsequently create renderings to visualize the entire sample in 1D (single projection) or 3D (full projection).  Full projections can be viewed in animation and exported as a movie file.

Rat retinal wholemount in which retinal ganglion cells were infected with rAAV/GFP.  Images were collected at 0.37um intervals with the 488nm laser to create a stack in the Z axis. Gallery mode allows the user to view all the images collected in the Z stack in a single display. Image courtesy of Mary Ellen Pease and Harry Quigley.	This is a 1D rendering made from the Z stack using the 3D software option.  Note the ability to follow retinal ganglion cell processes that would otherwise be out of focus in a conventional single plane image.	This is a movie of the full projection from the Z stack using the 3D software option. View movie: Windows Media: Dialup | Broadband

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