Zeiss LSM 510 Meta Confocal and Zeiss LSM 510 Meta Offline Workstation
Co-localization of multiple proteins and/or dyes
By virtue of the multitrack acquisition feature (sequential rather than simultaneous scanning with multiple laser lines) and the availability of the polychromatic meta detector, one can image multiple fluorophores within the same sample. Also key to this process is the use of emission fingerprinting to unmix spectrally close emission spectra.
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| Endothelial cells co-cultured with angioblasts. Endothelial cells were labeled with Cell Tracker Green and were imaged with the 488nm laser (green), Acetylated LDL was labeled with DiI and imaged with the 543nm laser (red), and angioblasts were labeled with Cell Tracker Far Red and imaged with the 633nm laser (blue). The composite image is located in the lower right corner of the frame. The multitrack acquisition mode was used to scan each laser separately and thus minimize cross talk. Image courtesy of Tarl Prow and Jerry Lutty. | Merged version of endothelial cells co-cultured with angioblasts. | Mature chicken retina. Presynaptic terminals were labeled with an antibody against synapse associated protein (SAP97) and visualized with Alexa 488 using the 488nm laser (green). Post-synaptic structures were labeled with an antibody directed against the glutamate receptor and visualized with Alexa 546 using the 543nm laser (red). Image courtesy of Karl Wahlin and Ruben Adler. |
