Hitachi H7600 TEM with Digital and Film Cameras

The Hitachi H7600 Transmission Electron Microscope comes with the following features:

• Dual AMT CCD camera system
  • 1K x 1K camera for real time view, search, autofocus
  • 2K x 2K 16-bit camera for digital micrographs
• Standard 3 ½ by 4 inch film camera also available
• 20-120 kV operating voltage
• Liquid nitrogen cooled anti-contamination device
• High contrast and high resolution imaging modes
• 60 degree high tilt, rotating motorized goniometer stage for use with tomography software
• Automontage function for both film and digital cameras.
• Multiple and single sample holders available.
• Free image J software with plug in for image analysis of AMT digital images available. (Plug in reads proprietary AMT calibration data saved with image file)

	Rat mossy fibers were retrogradely labeled with electron dense biotinylated dextrane amine (BDA), allowing visualization of the terminals in the granule cell domain of the lateral reticular and spinal trigeminal nucleus of the brainstem.  Image courtesy of Tan Pongstaporn and David Ryugo.
	Montage of a large mossy fiber terminal taken using the AMT automontage function and Panavue software for automatic assembly and registration of montage frames.  Image courtesy of Tan Pongstaporn and David Ryugo.
	Toxoplasma gondii organisms causing extensive degeneration of the retina. A pseudocyst contains several organisms (tachyzoites) which are bounded by a double layer pellicle.  The organisms are at various proliferative stages and contain numerous electron dense membrane-bound lysosomes and elongate cord-like structures (micronemes).  Image courtesy of Aida de la Cruz and WR Green.
	In a sampled area of a cellular membrane adjacent to the inner surface of the retina, fibrous astrocytes with a basal lamina, several junctional densities and numerous bundles of intermediate filaments is adjacent to a layer of newly synthesized collagen. Image courtesy of Aida de la Cruz and WR Green.

Hitachi manuals for downloading

• PDFs of these manuals are available on CD from the facility staff.

AMT (TEM digital cameras) manuals for downloading

• Everyday manual
• Measurement manual
• Panavue manual
• Montage manual

ImageJ analysis software

• ImageJ manual  Page 3 of the installation manual describes how to load ImageJ with plugin on your PC workstation using the installer below.
• Installer for ImageJ with plugin to read AMT calibration data
• Download at the National Institutes of Health
  This site has additional plugins for ImageJ.