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Tuesday, February 13, 2018

Glycoengineering in CHO: Working Inside and Out

Michael Betenbaugh and Qiong Wang

Department of Chemical and Biomolecular Engineering
Johns Hopkins Whiting School of Engineering

Pizza at 6 pm, Seminar at 6:30 pm
Johns Hopkins School of Medicine
612 Physiology Building, 725 N. Wolfe Street, Baltimore, MD 21205

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PAST PERFORMANCES (Click here for 1998-2003)

December 5 (2017) "6th Annual Area-wide Glycobiology Poster Session"

October 10 (2017) "Catalytic antibodies targeting glycans and glycoproteins in infection and immunity" by Maggie Wear, JHSPH

September 12 (2017) "Stressed Cells Crave Carbs too!" Natasha Zachara, JHU-SOM

April 18 (2017) "Acute increase in O-GlcNAc improves survival in mice with LPS-induced endotoxemia" by Rita Tostes, University of Sao Paulo, Sao Paulo, Brazil

January 10 (2017) "Comprehensive analysis of protein glycosylation by mass spectrometry" by Hui Zhang, David Clark, Punit Shah and Joseph Mertz, JHU-SOM

December 13 (2016) ""Deck the Peptidoglycan" - Metabolic carbohydrate probes for bacterial cell wall remodeling" by Catherine Leimkuhler Grimes, University of Delaware

October 11 (2016) "Galectins as pattern recognition receptors: First barrier or Trojan horse?" by Kelsey Abernathy, Chiguang Feng, and Gerardo Vasta, Univ of Maryland Sch of Med

September 13 (2016) "Glycoengineering Strategies to Treat Pancreatic and Brain Cancer" Kevin Yarema, Christopher Saeui, and Sagar Shah, Johns Hopkins University

May 10 (2016) "5th Annual Area-wide Glycobiology Poster Session"

April 12 (2016) "A 'Little Sugar' goes a long way" by John Hanover, Laboratory of Cell and Molecular Biology, NIDDK, NIH, Bethesda, MD

March 8 (2016) "Siglec-8, Siglec-9 & glycans that control human lung inflammation" by Ron Schnaar, Steve Fernandes, Anabel Gonzalez-Gil, & Ryan Porell, Dept. of Pharmacology, JHU-SOM

January 12 (2016) "Bacterial Polysaccharide Vaccines" by C. Allen Bush, University of Maryland Baltimore County

November 10 (2015) "Galectin-3 and cancer: Basic to translational" Hafiz Ahmed, Glycomantra

September 8 (2015) "Capsular polysaccharide biosynthesis: A tale of two transferases" by Willie Vann & Shonoi Ming, Laboratory of Bacterial Polysaccharides CBER/FDA, Bethesda, MD

May 14 (2015) "4th Annual Area-wide Glycobiology Poster Session"

April (2015) "Glycosphingolipids: a new culprit in atherosclerotic heart disease" by Subroto Chatterjee & Sumita Mishra, Dept. of Pediatrics, JHU-SOM

March 10 (2015) "Hydrogen bonding and structure in glycans" by Daron Freedberg, FDA/CBER, Bethesda, MD

February 10 (2015) "RosettaCarbohydrate: Expanding the computational tools available to glycoscientists" Jason Labonte, Johns Hopkins University

January 13 (2015) "Cross-Species Transplantation: Improved Control of Coagulation Dysregulation for Lung and Liver" by Agnes Azimzadeh, Department of Surgery, Univ of Maryland Sch of Med

December 9 (2014) "Serindipity in Glycobiology" Yuan Chuan Lee, Johns Hopkins University

October 14, (2014) "Glycosylation and Cancer: A Driver of Disease Progression or Achilles’ Heel?" by Mohit Mathew, Christopher Saeui and Kevin Yarema, Dept. of Biomedical Engineering, Johns Hopkins University

September 9 (2014) "Protein O-glycosylation in developmentally regulated secretion" by Kelley Ten Hagen, Developmental Glycobiology Section, NIDCR/NIH

May 16 (2014) "3rd Annual Area-wide Glycobiology Poster Session"

April 8 (2014) "Development of a glycoconjugate vaccine for cholera using squaric acid chemistry" by Peng Xu and Paul Kovac,NIH, NIDDK, Bethesda, MD

March 25 (2014) "Antigenic glycopeptides: Conformational impact of protein glycosylation and disease-relevant antigenic structure " by Shin-Ichiro Nishimura, Hokkaido University, Sapporo, Japan

March 11 (2014) "Glycoengineering for improved antibody therapy and HIV vaccine design" by Lai-Xi Wang, Mohammed Amin, John Giddens, and Joseph Lomino, University of Maryland School of Medicine

February 11 (2014) "The effects of calcium on the three-dimensional structure of sialic acid oligomers" by Daron Freedberg, FDA/CBER, Bethesda, MD

January 14 (2014) "CD24: A sweet double life in immunology and cancer biology" by Yang Liu, Children’s National Medical Center, Washington, DC

October 8 (2013) "Structural & functional analyses of protein glycosylation by mass spectrometry" by Hui Zhang, Shadi Toghi Eshghi, Weiming Yang & Paul Aiyetanatasha, Department of Pathology, JHU-SOM

September 10 (2013) "O-GlcNAc and OGT: Enzymatic and Non-Enzymatic Regulation of Survival Signaling" by Natasha Zachara, JHU-SOM

May 14 (2013) "2nd Annual Area-wide Glycobiology Poster Session"

April 9 (2013) "Chemical tools to study the activation of the intracellular innate immune protein Nod2" by Catherine Grimes, University of Delaware, Newark, DE

March 12 (2013) "Integrating engineering and biology in cancer research" by Konstantinos Konstantopoulos, Johns Hopkins University

January 8 (2013) "Affinity proteomics strategies to target carbohydrate-dependent host-pathogen interactions & Analytical platforms for native glycan arrays" by John Cipollo, Center for Biologics Evaluation & Research, US FDA, Bethesda, MD,

December 11 (2012) "Raising the Roof: Mechanisms by which tumor-associated lipids suppress innate immunity" by Tonya Webb, University of Maryland School of Medicine

October 9 (2012) "LID-PEG, Harnessing sugars to fight asthma and COPD" by Ron Schnaar, Department of Pharmacology and Molecular Science, JHU-SOM

September 11 (2012) "Metabolic Glycoengineering: New Opportunities and Old Mysteries" by the Kevin Yarema Group, Johns Hopkins University

May 8 (2012) "1st Annual Area-wide Glycobiology Poster Session"

April 17 (2012) "Adhesion is an important regulator of stem cell metabolism" by Roselle Abraham,  Department of Medicine, JHU-SOM

March 13 (2012) "Bacterial polysaccharide assembly and its application to vaccine synthesis" by Willie F. Vann & Pumtiwitt McCarthy, CBER/FDA, Bethesda, MD

February 7 (2012) "O-GlcNAc cycling: A systems biology approach" by John Hanover, Laboratory of Cell and Molecular Biology, NIDDK, NIH, Bethesda, MD

January 10 (2012) "Defining and Exploiting the Cell-Environment Interface" by Jennifer Van Eyk, Department of Medicine, JHU-SOM

December 13 (2011) "Artificial cells: new technology for the production of vaccines against bacterial pathogens" by Philip R. DeShong, Department of Chemistry & Biochemistry, University of Maryland, College Park, Maryland

October 11 (2011) "The role of glycan antigens in the immunobiology of schistosomiasis" by Anthony Kwame Nyame, University of Maryland Eastern Shore, Princess Anne, MD

September 13 (2011) "NMR Studies of Polysaccharides ON and OFF cells" by Daron Freedberg, FDA, Rockville, MD

May 10 (2011) "Lactosylceramide synthase: where Glycobiology meets Atherosclerosis" by Subroto Chatterjee, Department of Pediatrics, JHU-SOM

April 12 (2011) "Lipoplex delivery of ManNAc and sialic acid to rescue hyposialylation in a mouse model of HIBM" by Marjan Huizing & Tal Yardeni,  NHGRI, NIH, Bethesda, MD

March 8 (2011) "Structures and functions of cyanobacterial lectins and their effects on viral entry" by Carole Bewley, NIDDK, NIH, Bethesda, MD

February 8 (2011) "What we know that we do not know about malaria glycobiology (and what we intend to do about it)" by Rhoel R. Dinglasan, JHU-SPH

January 18 (2011) "Systems Glycobiology from the Glycome to the Genome" by Pauline Rudd, Dublin-Oxford NIBRT, Dublin, Ireland

December 14 (2010) "Sialic Acid Modulation of the Innate Immune Response" by Alan S. Cross, Center for Vaccine Development, Department of Medicine, University of Maryland School of Medicine 

Oct. 12 (2010) "Glycoproteins Made to Order. From Synthetic Chemistry to Glycobiology" by Lai-Xi Wang, Mohammed Amin, Jared Orwenyo, and Wei Huang,  Inst. of Human Virology, Univ. of MD.

Sept. 14 (2010) "Extensive crosstalk between O-GlcNAcylation & phosphorylation in nearly every cell process – Why???" by Gerald Hart, Dept. Biological Chemistry, JHU-SOM

May 11 (2010) "N-Glycosylation of E Cadherin Regulates Intercellular Adhesion" by Mihai Nita-Lazar, University of Maryland School of Medicine

April 13 (2010) "Glycoproteomics and Glycomics: A New Paradigm for Molecular Analysis of Disease Progression" by Hui Zhang, Yuan Tian, & Yan Li, Dept. of Pathology, JHU-SOM

March 9 (2010) "Design of Neoglycoprotein Arrays and Application to Cancer Vaccine Research" by Jeff Gildersleeve, Chemical Biology Lab, Center for Cancer Research, NCI, Frederick

Jan. 12 (2010) "GLUCOSAMINE: Inspiring New Treatments for Osteoarthritis and Cartilage Tissue Engineering" by Jennifer Elisseeff, Matthew Gibson & Jeanniene Coburn, Dept. Biomed. Eng., JHU

Dec. 8 (2009) "Structure/Activity Studies of APF: A Glycopeptide Antiproliferative Factor from Interstitial Cystitis Patients" by Joseph Barchi, NCI, Frederick, MD

Oct. 13 (2009) "O-GlcNAc, a Novel Regulator of the Cellular Stress Response and Cell Survival" by Natasha Zachara, Dept. Biological Chemistry, JHU-SOM

Sept 8 (2009) "Structure-function studies on galactosyltransferases and their use in bioconjugation - a progress report" by Pradman K. Qasba, Structural Glycobiology Section, NCI – Frederick

May 12 (2009) "Biosynthesis and functions of sialic acids" by The Schnaar Lab, Dept. of Pharmacol. & Mol. Sci., JHU-SOM

April 14 (2009) "Chemoenzymatic synthesis of glycoproteins for functional studies" by Lai-Xi Wang, Institute of Human Virology, Univ. of Md.

March 5 (2009) "GlycoBlotting: PCR for Glycobiology?" by Shin Nishimura, Hokkaido Univ., Sapporo, Japan

Feb 10 (2009) "Sugar-based drug discovery by modulating gene expression: The hexosamine platform" by Kevin Yarema, Dept. Biomed. Eng., JHU

Jan 13 (2009) "The Road to AvioGlycomics and Beyond" by Y.C. Lee, Biol. Dept., JHU.

Dec. 9 (2008) "Spreading the neus: the role of sialic acid modulation in inflammation" by Alan Cross, Dept. Med., Univ. Md

Oct. 14 (2008) "Vascular Adhesion of Colon Carcinoma Cells: Roles of Sialofucosylated CD44 and Carcinoembryonic Antigen" by K. Konstanopoulos, JHU

Sept 9 (2008) "Modeling glycan processing in normal and tumor cells" by F. Krambeck, M. Betenbaugh, S. Bennum, & K. Yarema, Depts of Che & Biol. Eng., and BME, JHU.

May 13 (2008) "Enzyme I of the Bacterial Phosphotransferase System, a Potential Global Regulator" by Drs. Saul Roseman, Himat Patel, & Kavita Vyas, Dept. of Biology, JHU

April 8 (2008) "N-Glycosites as molecular signatures of extracellular proteins for different cell types and diseases" by Hui Zhang, JHMI

Mar 11 (2008) "A Rosetta Stone for the O-GlcNAc code: Worms, Flies, Mice and Human Diseases" by Dr. John Hanover, NIH.

Feb. 12 (2008) " Global Profiling of Glycosylation Using Protein Chip Approach" by Dr. Heng Zhu, Dept. of Pharmacol. & Mol. Sci. & HIT Center, JHU-SOM

Jan. 8 (2008)  "Galectins as mediators of  developmental and immune mechanisms: Zebrafish and oyster as alternative animal models to elucidate their biological roles" by Dr. Gerardo Vasta, COMB, Univ. Md, Baltimore, MD

Dec.  4 (2007) "The Sweet Nature of the Cellular Stress Response" by Natasha Zachara, JHMI.

Oct. 9 (2007) "In Pursuit of Carbohydrate-Based HIV Vaccine" by Lai-xi Wang, Inst. Human Virol., Univ. of Md.

Sept. 11 (2007) " Polymerization of sialic acid by bacterial polysialyltransferases" by Willie Vann, FDA/CBER, Bethesda, MD

April 19 (2007) "Glycobiology of Leishmania Parasites" by Dr. Sam Turco (Univ. of Kentucky).

March 20 (2007) "Glycobiology of Single Cells: Enzyme assays and glycolipid metabolomics" by Drs. M. Palcic and O. Hindsgaul, Carlsburg Lab., Denmark.

Feb. 20 (2007) "Molecular Basis of Streptococcal Receptor Polysaccharide Structure and Function" by John Cisar, NIH/NIDCR (Bethesda, MD)

Jan 16 (2007) "Glyconanotechnology:Synthesis, Characterization and Therapeutic Potential of Sugar-coated Nanoparticles" by J. Barchi (NCI, Frederick, MD)

Dec. 14 (2006) "The Malaria Parasite's Proclivity for Sweets: Role of Protein-Glycan Interactions in Plasmodium Host Cell Invasion" by R. R. Dinglasan (JHSPH)

Nov 21 (2006) "All the neus that is fit to print:The role of Neu1 and Neu3 sialidases in the immune system" by N. Stamatos (UM, IHV)

Oct 17 (2006) "How do sugars modulate Notch activity" by R. Haltiwanger (Stoney Brook Univ.)

Sept. 7 (2006) "Hi-Speed Screening of Total Glycans in Cancer Cells" by K. Kakehi (Kinki Univ.)

May 9 (2006) ""GPI Anchoring of Fungal Proteins:Challenges of Studying Membrane-Bound Proteing" by Tamara Hendrickson (Dept. Chem., JHU)

April 18 (2006) "Harnessing glycobiology to help in nerve repair" by Ron Schnaar et al. (JHUSOM)

March 21 (2006) "An O-Glycosyltransferase is Required During Multiple Stages of Drosophila Development"  by Kelly Ten Hagen (Developmental Glycobiology Unit, NIDCR/NIH)

Feb. 14 (2006) "Sialic acid: A regulator of apoptosis, proliferation, and differentiation" by K.Yarema (BME, JHU)

Jan. 10 (2006) "Structure and Function of b-1,4-Gal-transferase family members: Structure-based design of new glycosyltransferases" by P. K. Qasba, E.Boeggeman & B.Ramakrishnan (NIH/NCI)

Dec. 13 (2005) "Lectin Repertories in Invertebrates and Ectothermic Vertebrates:Their Roles in Embryogenesis and Innate Immunity¨ by Gerardo Vasta (COMB)

Nov. 15 (2005) "Dynamic Interplay Between O-GlcNAc and O-phosphate:Roles in Transcription, Apoptosis, and the Cell Cycle" by Hart, Houskey, Butkinaree, & Slawson (JHUMI)

Oct. 11 (2005) "Bioorganic synthesis of glycopeptides and HIV-1 vaccine design" by Lai-xi Wang (IHV, Univ. of Md.)

May 24 (2005) "Glucose Uptake by Escherichia coli (Pursuit of an artefact)" by K. Vyas, H. Patel, & S. Roseman

April 20 (2005) "Catch-bond mechanism of saccharide-specific bacterial adhesion" by Evgeni Sokurenko (Dept. Microbiology, Univ. Of Washington)

March 15 (2005)"Siglec-8 -- A lectin regulator of allergic inflammation with unique glycan binding specificity" by Bruce Bochner (Div. Clin. Immun. JH Asthma and Allergy Center) & Ron Schnaar (Dept Pharm. & Mol. Sci., JHMS)

Feb. 16 (2005) "Mining Structural Details by MSn and Automation" by Vernon Reinhold, Univ. of New Hampshire

Jan. 11 (2005) "Fluorophore-Assisted Carbohydrate Electrophoresis Studies of CDG-Ia: A SLOw about-FACE"  by Dr. Mark Lehman, Dept of Pharmacol., Univ. of Texas Southwestern Med. Ctr.

Dec. 14 (2004) "C-Reactive Protein: not for Phosphorylcholine only" by Reiko Lee & Y.C.Lee Dept. of Biology (JHU)

Oct. 12 (2004) "Metabolic connections between sialic acid biosynthesis, human disease, and apoptosis" by Kevin Yarema, Dept. of Biomed. Eng. (JHU)

May 18 ( 2004) -- " The Sweet Kiss of Death: Galactin-1, Autoimmunity and Tumor Escape", Gabriel Rabinovich, Laboratorio de Inmunogenetica Hospital de Clinicas "José de San Martín", Facultad de Medicina Universidad de Buenos Aires

April 20 (2004) -- " Metabolic Pathways of Glycosylation in Mammalian & Insect Cells" by  Julian Jones, Karthik Viswanathan S. Krag, M. Betenbaugh, Dept of Chem & Biomol Eng (JHU) & Dept of Biochem & Mol Biol (JHSPH) Baltimore, MD

Mar. 23 (2004) -- "What is the meaning of serotype?--- The differing structures of O-polysaccharide chains of E. coli O86" by Dr. Allen Bush, UMBC, Baltimore, MD.

Feb. 10 (2004) -- "Potential Role of Sialidase(s) of PBMCs in Influencing Cell Function and Infectivity of HIV-1" by Dr. Nicholas Stamatos, Inst. of Human Virology, Univ. of Md., Biotech. Institute. Baltimore, MD 21201

Jan. 13 (2004) -- "Selectin Ligands -- Men are not (always) mice" by R. Schnaar, Dept. of Pharmacology & Molecular Sciences, JHU-SOM Baltimore, MD