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Andrea A. Zachary, Ph.D.

Professor of Medicine

See Research on Pubmed



  • Professor
  • Director, Division of Immunogenetics and Transplantation Immunology
  • Director, Immunogenetics Laboratory
  • International HLA Councillor
  • Advisory Board, Hong Kong Society for Histocompatibility and Immunogenetics
  • Professor of Medicine

Centers & Institutes

  • McKusick-Nathans Institute of Genetic Medicine

Technology Expertise

HLA antibody characterization, population genetics, allograft rejection


Dr. Zachary is a director of the Division of Immunogenetics and Transplantation Immunology in the Department of Medicine. Dr. Zachary oversees clinical histocompatibility testing for solid organ and tissue transplantation.

Her major interest is in the humoral response to allogeneic transplantation. She was involved in the development of the protocol used at Johns Hopkins to desensitize potential transplant recipients who are immunologically incompatible with their donor. She initiated and participated in the development of an assay for the isolation and quantification of HLA-specific B cells.  

Currently, she has expaned her investigation into non-HLA antibodies relevant to transplantation. Dr. Zachary has been active in several national and international medical and scientific organizations and is a recipient of the Distinguished Scientist Award of the American Society for Histocompatibility and Immunogenetics and the Ohio State University Alumni Association Professional Achievement Award.


  • English
Additional Resources +
  • Education +


    • BSc, Ohio State University, 1967
    • MS, Ohio State University, 1969
    • Ph.D, Case Western Reserve university, 1982
  • Research & Publications +

    Research Summary

    Dr. Zachary's major research is in the mechanisms of desensitization and the role of non-HLA antibodies in transplantation.

    Selected Publications View all on PubMed

    Zachary AA, Lucas DP, Montgomery RA, Leffell MS. Rituximab Prevents an Anamnestic  Response in Patients  with Cryptic Sensitization to HLA.  Transplantation, 2013;95:701-4.

    Gladstone DE, Zachary AA, Fuchs EJ, Luznik L, Kasamon YL, King KE, Brodsky RA, Jones RJ, Leffell MS. Partially Mismatched Transplantation and HLA Donor Specific Antibodies.  Biol Blood Marrow Transplant. 2013; 19:647-652.

    Alachkar N, Lonze BE, Zachary AA, Holechek MJ, Schillinger K, Cameron AM, Desai NM, Dagher NN, Segev DL, Montgomery RA, Singer AL. Infusion of high-dose intravenous immunoglobulin fails to lower the strength of human leukocyte antigen antibodies in highly sensitized patients. Transplantation. 2012;94:165-71.

    Zachary AA, Vega RM, Lucas DP, Leffell MS. HLA antibody detection and characterization by solid phase immune assays: methods and pitfalls. Methods Mol Biol. 2012; 882:289-308.

    Callender CJ , Fernandez-Vina M, Leffell MS, Zachary AA. Frequency of HLA-DP-specific antibodies and a possible new cross reacting group.  Hum immunol. 2012;73: 175-9.

    Zachary A, Reinsmoen NL: Quantifying HLA-Specific antibodies in patients undergoing desensitization. Curr Opin Organ Transplant. 16: 410-415, 2011

    Montgomery RA, Lonze BE, King KE, Kraus ES, Kicirka LM, Locke JE, Warren DS, Simpkins CE, Dagher NN, Singer AL, Zachary AA, Segev DL: Desensitization in HLA-incompatible kidney recipients and survival. N Engl J Med. 365:318-26, 2011

    Zachary AA, Eng HS: Desensitization: Achieving Immune Détente. Tissue Antigens 77: 3-8, 2011.

    Zachary AA, Sholander JT, Houp JA, Leffell MS: Using real data for virtual crossmatch. Human Immunology  70: 574-9, 2009.

    Kopchaliiska D, Zachary AA, Montgomery RA, Leffell MS: Reconstitution of peripheral allo-specific CD19+ B cell subsets after B lymphocyte depletion therapy in renal transplant patients. Transplantation 87: 1394-401, 2009.

    Zachary AA, Montgomery RA, Leffell MS: Defining unacceptable HLA antigens. Current Opinion Organ Transplant 13: 405-10, 2008.

    Zachary AA, Kopchaliiska D, Montgomery RA, Leffell MS: HLA-Specific B cells: I A method for their detection, quantification, and isolation using HLA tetramers.Transplantation 15: 982-8, 2007.

    Zachary AA, Kopchaliiska D, Montgomery RA, Melancon JK, Leffell MS: HLA-Specific B cells: II Application to Transplantation. Transplantation 83: 989-994, 2007.


    The laboratory has an ongoing investigation on non-HLA targets of rejection that are expressed on vascular endothelial cells. This research is being conducted under the direction of Dr. Annette Jackson.   Dr. Mary Carmel Philogene is investigating the role of AT1R antibody in organ transplantation.   Dr. Mary S. Leffell who co-directs both the division and the laboratory, is involved in studying desensitization in haplo-identical hematopoietic stem cell transplantation.
  • Academic Affiliations & Courses +
  • Activities & Honors +


    Rose Payne Award, American Society for Histocompatibility and Immunogenetics, 2013
    Distinguished Scientist Award, American Society for Histocompatibility and Immunogenetics, 2010
    Professional Achievement Award, The Ohio State University Alumni Association, 2001
    Distinguished Service Award, American Society for Histocompatibility and Immunogenetics, 2000
    Top Cited Articles, Elsevier, 2008-2010

    Professional Activities

    American Society of Transplantation, Diagnostic Community of Practice, 2011

    International HLA Councillor, 2008

    International Advisor, Hong Kong Society for Histocompatibility and Immunogenetics, 2011

    Associate Editor, Clinical Transplantation, 2010

    Editorial Board Member: Transplantation, Human Immunology, Transgene, Transplant Coordination

  • Videos & Media +

    Lectures and Presentations

    AT1R in Lung Transplantation
    Joint HKSHI-APHIA Scientific Meeting
    Hong Kong
    July 31, 2013

    The Role of Non-HLA Antibodies in Transplantation. 
    Joint HKSHI-APHIA Scientific Meeting
    Hong Kong
    August 1, 2013

    Finding the Route for Transplanting the Sensitized Patient.  
    Joint HKSHI-APHIA Scientific Meeting
    Hong Kong
    July 31, 2013

    Monitoring for DSA: Cutoffs and Triggers For Treatment           
    Antibody Mediated Rejection In Liver Transplantation
    Dallas TX
    March 22-23,2013

    Antibodies and Transplantation                                                       
    Pathology Grand Rounds, Choices, Challenges, and Changes.           
    Temple University , Philadelphia, PA
    January 25, 2013

    Who Should Be Desensitized and How.                                     
    XXIVth International Congress of the Transplantation Society
    Berlin, Germany
    July 19, 2012

    The Crossmatch: Obligatory or Optional                              
    Education Session of the European Federation for Immunogenetics                                                                       
    Liverpool, England
    May 30-June 2,2012

    Report of the 16th International HLA and Immunogenetics Workshop
    Liverpool, England
    May 30- June 2,2012

    Antigen Presentation: The Role of HLA Molecules in Cellular Interactions
    31st Current Course on Immunogenetics    
    INDRE- Department of Immunogenetics
    Mexico City, Mexico.
    July 25- August 5, 2012

    Keynote Address: Multiplex  Antibody Assays: Unleashing the Power                                                                                         
    APHIA Annual Scientific Meeting
    Brisbane, Australia
    November 16, 2011

  • Events +
  • Contact & Locations +


    • Medicine - Immunogenetics and Transplant Immunology

    For Research Inquiries Contact

    Phone: (410) 614-8978
    Fax: (410) 955-0431
    2041 E Monument St.
    Baltimore, MD 21205

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