Dr. Zachary's major research is in the mechanisms of desensitization and the role of non-HLA antibodies in transplantation.
HLA antibody characterization, population genetics, allograft rejection
The laboratory has an ongoing investigation on non-HLA targets of rejection that are expressed on vascular endothelial cells. This research is being conducted under the direction of Dr. Annette Jackson. Dr. Mary Carmel Philogene is investigating the role of AT1R antibody in organ transplantation. Dr. Mary S. Leffell who co-directs both the division and the laboratory, is involved in studying desensitization in haplo-identical hematopoietic stem cell transplantation.
Zachary AA, Lucas DP, Montgomery RA, Leffell MS. Rituximab Prevents an Anamnestic Response in Patients with Cryptic Sensitization to HLA. Transplantation, 2013;95:701-4.
Gladstone DE, Zachary AA, Fuchs EJ, Luznik L, Kasamon YL, King KE, Brodsky RA, Jones RJ, Leffell MS. Partially Mismatched Transplantation and HLA Donor Specific Antibodies. Biol Blood Marrow Transplant. 2013; 19:647-652.
Alachkar N, Lonze BE, Zachary AA, Holechek MJ, Schillinger K, Cameron AM, Desai NM, Dagher NN, Segev DL, Montgomery RA, Singer AL. Infusion of high-dose intravenous immunoglobulin fails to lower the strength of human leukocyte antigen antibodies in highly sensitized patients. Transplantation. 2012;94:165-71.
Zachary AA, Vega RM, Lucas DP, Leffell MS. HLA antibody detection and characterization by solid phase immune assays: methods and pitfalls. Methods Mol Biol. 2012; 882:289-308.
Callender CJ , Fernandez-Vina M, Leffell MS, Zachary AA. Frequency of HLA-DP-specific antibodies and a possible new cross reacting group. Hum immunol. 2012;73: 175-9.
Zachary A, Reinsmoen NL: Quantifying HLA-Specific antibodies in patients undergoing desensitization. Curr Opin Organ Transplant. 16: 410-415, 2011
Montgomery RA, Lonze BE, King KE, Kraus ES, Kicirka LM, Locke JE, Warren DS, Simpkins CE, Dagher NN, Singer AL, Zachary AA, Segev DL: Desensitization in HLA-incompatible kidney recipients and survival. N Engl J Med. 365:318-26, 2011
Zachary AA, Eng HS: Desensitization: Achieving Immune Détente. Tissue Antigens 77: 3-8, 2011.
Zachary AA, Sholander JT, Houp JA, Leffell MS: Using real data for virtual crossmatch. Human Immunology 70: 574-9, 2009.
Kopchaliiska D, Zachary AA, Montgomery RA, Leffell MS: Reconstitution of peripheral allo-specific CD19+ B cell subsets after B lymphocyte depletion therapy in renal transplant patients. Transplantation 87: 1394-401, 2009.
Zachary AA, Montgomery RA, Leffell MS: Defining unacceptable HLA antigens. Current Opinion Organ Transplant 13: 405-10, 2008.
Zachary AA, Kopchaliiska D, Montgomery RA, Leffell MS: HLA-Specific B cells: I A method for their detection, quantification, and isolation using HLA tetramers.Transplantation 15: 982-8, 2007.
Zachary AA, Kopchaliiska D, Montgomery RA, Melancon JK, Leffell MS: HLA-Specific B cells: II Application to Transplantation. Transplantation 83: 989-994, 2007.