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Herschel V. Wade, Ph.D.

Photo of Dr. Herschel V. Wade, Ph.D.

Assistant Professor of Biophysics and Biophysical Chemistry

Research Interests: Dissecting multidrug recognition, multidrug signaling and ligand-responsive allosteric mechanisms

Background

Dr. Herschel Wade is an assistant professor of biophysics and biophysical chemistry at the Johns Hopkins University School of Medicine. His research focuses on the structural and mechanistic aspects of multi-drug resistance, ligand-dependent molecular switches and how metal ion homeostasis may lead to new drugs to reverse multidrug resistance.

Dr. Wade graduated with a B.S. from California Polytechnic University and earned a Ph.D. at the University of California, San Francisco.

Dr. Wade and his lab are studying how biological systems read and transform chemical signals into a diverse range of biological outputs. To better understand the functional roles of ligand interactions in regulatory systems, his lab also focuses on BmrR and other MDR functions that are stimulated by chemically diverse small-molecules.

Dr. Wade has been recognized with the 2009 Beckman Young Investigator Award, an award that funds the high-risk, innovative research of brilliant young scientists.

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Titles

  • Assistant Professor of Biophysics and Biophysical Chemistry

Education

Degrees

  • B.S., California Polytechnic State University (California) (1994)
  • Ph.D., University of California (San Francisco) (California) (2000)

Research & Publications

Research Summary

Dr. Wade is interested in the emergence of structural genomics, proteomics and the large-scale sequencing of many genomes provides experimental access to regions of protein sequence-structure-function landscapes which have not been explored through traditional biochemical methods. Dr. Wade and his lab will use protein libraries, chemistry, biophysics, molecular biology and structural methods to examine the basis of molecular recognition in the context of several important biological problems, including structural and mechanistic aspects of multi-drug resistance, ligand-dependent molecular switches and metal ion homeostasis.

Lab

The Wade Lab would like to understand how biological systems read and transform chemical signals into a diverse range of biological outputs. To better understand the functional roles of ligand interactions in regulatory systems, the Wade laboratory focuses on BmrR and MDR functions that are stimulated by chemically diverse small-molecules. Biochemical, biophysical and x-ray crystallographic approaches are employed to dissect and examine ligand-directed effects at the binding, functional and structural levels. The aim is to combine functional, thermodynamic and structural studies into a more integrative analysis of ligand recognition and ligand-dependent control of protein conformation.

Lab Website: Herschel Wade Lab

Selected Publications

L.A. Brammer, J.M. Smith, H. Wade, C. Freel Meyers. 2011. "1-Deoxy-D-xylulose 5-phosphate synthase: A new twist in thiamine diphosphate-dependent enzymology." J. Biol. Chem. in the press, doi:10.1074/jbc.M111.259747

S. Bachas, C. Eginton, D. Gunio, H.Wade. "Contributions to multidrug recognition in BmrR." PNAS 108(27) 11046-11051. 2011.

H. Wade. "MD recognition by MDR gene regulators Current Opinion in Structural Biology" 20(4): 489-496. 2010.

S. Hu, Z. Xie, A. Onishi, X. Yu, L. Jiang, J. Lin, H.S. Rho, C. Woodard, H. Wang, J.S. Jeong, S. Long, X. He, H. Wade, S. Blackshaw, J. Qian, and H. Zhu. "Profiling the human protein-DNA interactorne reveals ERK2 as a transcriptional repressor of interferon signaling." Cell 139(3): 610-622. 2009

H. Wade, S. Stayrook, W.F. DeGrado "Diferric structure of a model diiron protein; implications for cofactor stabilization and catalysis." Angew. Chem. Int. Ed. 45(30): 4951.4954. 2006.

Academic Affiliations & Courses

Graduate Program Affiliation

Biochemistry, Cellular and Molecular Biology Graduate Program

Activities & Honors

Honors

  • Beckman Young Investigator Award, 2009
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